Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,075,237 |
T→C |
I316V (ATA→GTA) |
yggF ← |
fructose 1,6‑bisphosphatase YggF |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,075,237 | 0 | T | C | 100.0%
| 91.6
/ NA
| 26 | I316V (ATA→GTA) | yggF | fructose 1,6‑bisphosphatase YggF |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/12); total (14/12) |
GCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTT > NC_000913/3075162‑3075312
|
gCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCtattacatta > 1:534032/1‑80 (MQ=255)
gCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCtattacatta < 1:533982/80‑1 (MQ=255)
cTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTAttacattaca > 2:534028/1‑81 (MQ=255)
aaGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTacac < 1:533984/79‑1 (MQ=255)
aaGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTacac < 2:533983/79‑1 (MQ=255)
aaGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTacac < 1:533985/79‑1 (MQ=255)
aGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGtt < 1:533986/81‑1 (MQ=255)
cacaTCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTg < 1:533987/81‑1 (MQ=255)
catcatCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGACCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGt > 1:534056/1‑81 (MQ=255)
atcatcTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTc > 1:534043/1‑81 (MQ=255)
tcatcTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTcc < 1:533988/81‑1 (MQ=255)
atcTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCcgc > 2:534062/1‑81 (MQ=255)
tctGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCCGcgccg > 2:534030/1‑81 (MQ=255)
cAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGAt > 2:534039/1‑81 (MQ=255)
gCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGAt > 2:534031/1‑79 (MQ=255)
cGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGATCAg > 2:534024/1‑81 (MQ=255)
tCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGATCAGTa < 1:533989/81‑1 (MQ=255)
gCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGATCAGTAAt > 1:534050/1‑81 (MQ=255)
ttGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCt < 2:533990/81‑1 (MQ=255)
ccGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTc < 1:533991/78‑1 (MQ=255)
ccGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCg > 1:534045/1‑80 (MQ=255)
cGATAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCa < 2:533992/81‑1 (MQ=255)
tAGATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCAccc > 1:534034/1‑81 (MQ=255)
gATCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCa > 1:534052/1‑81 (MQ=255)
tCACCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCAtt > 2:534066/1‑81 (MQ=255)
aCCATCAATGCAGGGAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTc < 1:533993/80‑1 (MQ=255)
cAGGGAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGCCTGCGTCCGCACCCCATTCGCCGTCTGtt < 1:533994/80‑1 (MQ=255)
gAGTCTATTACATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATa > 1:534040/1‑81 (MQ=255)
cTATTACATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGATGGATACCGt > 1:534048/1‑81 (MQ=255)
tattacattaCACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGtt > 1:534055/1‑81 (MQ=255)
attacattacACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGtt > 2:534057/1‑80 (MQ=255)
|
GCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTT > NC_000913/3075162‑3075312
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
