Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,077,659 |
C→T |
A401T (GCA→ACA) |
cmtA ← |
mannitol‑specific PTS enzyme IICB component CmtA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,077,659 | 0 | C | T | 100.0%
| 77.3
/ NA
| 22 | A401T (GCA→ACA) | cmtA | mannitol‑specific PTS enzyme IICB component CmtA |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (11/11); total (11/11) |
CCAGACTGGCATGAGTAACGACGATATCCGCATCCGCAGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGCCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTCGCGCCCATCGCACTGGAGCCCATCCCCGCATCGCAAACA > NC_000913/3077584‑3077735
|
ccAGACTGGCATGAGTAACGACGATATCCGCATCCGCAGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGTCAGc < 2:573460/80‑1 (MQ=255)
cAGACTGGCATGAGTAACGACGATATCCGCATCCGCAGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGTCAGccc < 2:573461/81‑1 (MQ=255)
ggCATGAGTAACGACGATATCCGCATCCGCAGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGTCAGCCCCGCttt > 2:573495/1‑81 (MQ=255)
gCATGAGTAACGACGATATCCGCATCCGCAGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGTCAGCCCCGCttt < 2:573462/80‑1 (MQ=255)
aTGAGTAACGACGATATCCGCATCCGCAGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGTCAGCCCCGCTTTTTc > 1:573491/1‑81 (MQ=255)
aTGAGTAACGACGATATCCGCATCCGCAGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGTCAGCCCCGCTTTTTc > 2:573501/1‑81 (MQ=255)
gacgaTATCCGCATCCGCAGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGTCAGCCCCGCTTTTTCCAGGCGtt > 1:573504/1‑80 (MQ=255)
gacgaTATCCGCATCCGCAGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGTCAGCCCCGCTTTTTCCAGGCGtt > 2:573500/1‑80 (MQ=255)
gaTATCCGCATCCGCAGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGTCAGCCCCGCTTTTTCCAGGCGTTTACg > 2:573502/1‑81 (MQ=255)
tccgcatccgcaGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGTCAGCCCCGCTTTTTCCAGGCGTTTACGGaa < 1:573463/80‑1 (MQ=255)
ccgcaGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGTCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTcg > 2:573509/1‑81 (MQ=255)
cgcaGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGTCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTcg < 2:573465/80‑1 (MQ=255)
cgcaGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGTCAGCACCGCTTTTTCCAGGCGTTTACGGAAGGTGGTcgc < 1:573464/81‑1 (MQ=255)
ggCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGTCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTCGCGcc > 2:573510/1‑80 (MQ=255)
ttttCTATGGCGTAATGTTTTACTTCAATTGTCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTCGCGCCCATCGCa < 1:573466/81‑1 (MQ=255)
tCTATGGCGTAATGTTTTACTTCAATTGTCGGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTCGCGCCCATCGCACt < 2:573467/80‑1 (MQ=255)
cTATGGCGTAATGTTTTACTTCAATTGTCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTCGCGCCCATCGCACTg > 2:573505/1‑80 (MQ=255)
tGGCGTAATGTTTTACTTCAATTGTCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTCGCGCCCATCGCACTGGAg < 1:573468/80‑1 (MQ=255)
gCGTAATGTTTTACTTCAATTGTCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTCGCGCCCATCGCACTGGAGccc < 2:573469/81‑1 (MQ=255)
tttACTTCAATTGTCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTCGCGCCCATCGCACTGGAGCCCATCCCCGCa > 1:573513/1‑81 (MQ=255)
aTTGTCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTCGCGCCCATCGCACTGGAGCCCATCCCCGCATCGcaaaca < 2:573470/81‑1 (MQ=255)
aTTGTCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTCGCGCCCATCGCACTGGAGCCCATCCCCGCATCGcaaac > 1:573521/1‑80 (MQ=255)
|
CCAGACTGGCATGAGTAACGACGATATCCGCATCCGCAGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGCCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTCGCGCCCATCGCACTGGAGCCCATCCCCGCATCGCAAACA > NC_000913/3077584‑3077735
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
