Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,100,922 |
C→T |
L234L (CTG→CTA) |
yggN ← |
putative EcfF |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,100,922 | 0 | C | T | 100.0%
| 67.6
/ NA
| 20 | L234L (CTG→CTA) | yggN | putative EcfF |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (9/11); total (9/11) |
AAGAATTAAACGGCATAAAAAAGTATTATGCCGTCTTAAAATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTC > NC_000913/3100856‑3100996
|
aagaaTTAAACGGCATAAAAAAGTATTATGCCGTCTTAAAATACAGGATTATTTTAAATTCCCGACTAGGGCTTTGCGGCt > 1:539211/1‑81 (MQ=255)
aaTTAAACGGCATAAAAAAGTATTATGCCGTCTTAAAATAGAGGATTATTTTAAATTCCCGACTAGGGCTTTGCGGCTATc < 2:539192/81‑1 (MQ=255)
tAAACGGCATAAAAAAGTATTATGCCGTCTTAAAATAGAGGATTATTTTAAATTCCCGACTAGGGCTTTGCGGCTATCTTc > 2:539221/1‑81 (MQ=255)
aTAAAAAAGTATTATGCCGTCTTAAAATAGAGGATTATTTTAAATTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTca > 1:539214/1‑81 (MQ=255)
tattatGCCGTCTTAAAATAGAGGATTATTTTAAATTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGc < 1:539193/81‑1 (MQ=255)
ttatGCCGTCTTAAAATAGAGGATTATTTTAAATTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTa > 1:539213/1‑81 (MQ=255)
atGCCGTCTTAAAATAGAGGATTATTTTAAATTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTAc < 1:539194/80‑1 (MQ=255)
gCCGTCTTAAAATAGAGGATTATTTTAAATTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACaaa > 2:539216/1‑81 (MQ=255)
ccGTCTTAAAATAGAGGATTATTTTAAATTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAAc < 1:539195/81‑1 (MQ=255)
aaaaTAGAGGATTATTTTAAATTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCttt < 2:539198/80‑1 (MQ=255)
aaaaTAGAGGATTATTTTAAATTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCtt < 1:539196/79‑1 (MQ=255)
aaaaTAGAGGAATATTTTAAATTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCtt < 1:539197/79‑1 (MQ=255)
tAGAGGATTATTTTAAATTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCaa < 1:539199/81‑1 (MQ=255)
ggATTATTTTAAATTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAActg < 2:539200/81‑1 (MQ=255)
gATTATTTTAAATTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAActgc > 1:539217/1‑81 (MQ=255)
attattTTAAATTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAActgct < 1:539201/81‑1 (MQ=255)
aaTTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCtt > 2:539225/1‑81 (MQ=255)
aaTTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCt < 2:539202/80‑1 (MQ=255)
tCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCttttt > 2:539229/1‑81 (MQ=255)
cccGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTc > 1:539222/1‑81 (MQ=255)
|
AAGAATTAAACGGCATAAAAAAGTATTATGCCGTCTTAAAATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTC > NC_000913/3100856‑3100996
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
