Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,155,049 |
G→A |
T57M (ACG→ATG) |
yqhC ← |
DNA‑binding transcriptional activator YqhC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,155,049 | 0 | G | A | 100.0%
| 110.7
/ NA
| 32 | T57M (ACG→ATG) | yqhC | DNA‑binding transcriptional activator YqhC |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (11/21); total (11/21) |
ATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATT > NC_000913/3154974‑3155119
|
atatAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCg < 2:550187/81‑1 (MQ=255)
taACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGcc < 1:550188/80‑1 (MQ=255)
ttATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACaaa > 1:550224/1‑81 (MQ=255)
tGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGc < 1:550189/81‑1 (MQ=255)
gCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGca > 2:550226/1‑81 (MQ=255)
cGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACAt < 1:550190/81‑1 (MQ=255)
ggAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATc > 2:550230/1‑81 (MQ=255)
ggAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATc < 1:550191/81‑1 (MQ=255)
gAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCg < 2:550192/81‑1 (MQ=255)
agagAATTATGATGCCAGGCTCATACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGc > 1:550250/1‑80 (MQ=255)
gaATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCaa > 2:550232/1‑79 (MQ=255)
aaTTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAg < 2:550194/81‑1 (MQ=255)
aaTTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAg < 1:550193/81‑1 (MQ=255)
tATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTcc > 1:550222/1‑81 (MQ=255)
tgatgCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCt < 2:550195/80‑1 (MQ=255)
tgatgCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTg < 2:550196/81‑1 (MQ=255)
atgCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGa > 1:550223/1‑80 (MQ=255)
gCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTAt < 1:550197/81‑1 (MQ=255)
gTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTAttt < 1:550200/79‑1 (MQ=255)
gTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTAttt < 1:550199/79‑1 (MQ=255)
gTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTc > 1:550235/1‑81 (MQ=255)
gTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTc < 2:550201/81‑1 (MQ=255)
gTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTc < 1:550198/81‑1 (MQ=255)
aCATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCtt > 2:550234/1‑81 (MQ=255)
cATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCtt > 1:550228/1‑80 (MQ=255)
aTCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTa < 1:550202/81‑1 (MQ=255)
tCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTAt < 2:550203/81‑1 (MQ=255)
tgtACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAg < 1:550204/81‑1 (MQ=255)
tACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGtt < 1:550206/81‑1 (MQ=255)
tACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGtt < 1:550205/81‑1 (MQ=255)
cGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGttt < 2:550207/80‑1 (MQ=255)
gCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTttatt > 2:550231/1‑80 (MQ=255)
|
ATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATT > NC_000913/3154974‑3155119
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
