Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 524 31.4 1748232 97.8% 1709770 80.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,156,186 G→A G278S (GGT→AGT)  yqhD → NADPH‑dependent aldehyde reductase YqhD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,156,1860GA100.0% 181.2 / NA 51G278S (GGT→AGT) yqhDNADPH‑dependent aldehyde reductase YqhD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/25);  total (26/25)

ACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATACCAAGCGCGCTAA  >  NC_000913/3156112‑3156262
                                                                          |                                                                            
aCGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTg                                                                         >  2:550474/1‑80 (MQ=255)
  ggTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGAt                                                                      >  1:550481/1‑81 (MQ=255)
  ggTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGAt                                                                      >  1:550467/1‑81 (MQ=255)
       gATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCAcg                                                                  <  1:550406/80‑1 (MQ=255)
        aTTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCAcgcg                                                                >  1:550513/1‑81 (MQ=255)
        aTTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCAcgcg                                                                >  1:550466/1‑81 (MQ=255)
        aTTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCAcgc                                                                 <  2:550407/80‑1 (MQ=255)
              gcTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAaca                                                          >  2:550477/1‑81 (MQ=255)
              gcTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAaca                                                          >  1:550458/1‑81 (MQ=255)
                tGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACt                                                        >  2:550464/1‑81 (MQ=255)
                  gCGTACCGCAGGACTGGGCAACGCATATGCTGGGCGACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTg                                                       <  1:550408/80‑1 (MQ=255)
                   cGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGc                                                     >  1:550512/1‑81 (MQ=255)
                       ccGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTAt                                                  <  1:550409/80‑1 (MQ=255)
                       ccGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATc                                                 >  2:550484/1‑81 (MQ=255)
                       ccGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATc                                                 <  1:550410/81‑1 (MQ=255)
                        cGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCg                                                >  2:550462/1‑81 (MQ=255)
                        cGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCg                                                >  2:550473/1‑81 (MQ=255)
                           aGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTcc                                             >  1:550465/1‑81 (MQ=255)
                           aGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTcc                                             >  1:550471/1‑81 (MQ=255)
                             gACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTcctg                                           >  1:550479/1‑81 (MQ=255)
                              aCTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTcctgc                                          >  1:550470/1‑81 (MQ=255)
                              aCTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTcctgc                                          <  1:550411/81‑1 (MQ=255)
                                 gggCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTcctgcctg                                       >  2:550483/1‑81 (MQ=255)
                                 gggCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTcctgcctg                                       >  2:550515/1‑81 (MQ=255)
                                    cAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCAc                                    >  2:550501/1‑81 (MQ=255)
                                     aaCGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACt                                   <  2:550412/81‑1 (MQ=255)
                                          atatGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTgg                               >  2:550475/1‑80 (MQ=255)
                                          atatGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGa                              <  1:550413/81‑1 (MQ=255)
                                          atatGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGa                              <  2:550414/81‑1 (MQ=255)
                                          atatGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGa                              <  1:550415/81‑1 (MQ=255)
                                             tGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATg                           <  1:550416/81‑1 (MQ=255)
                                              gCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATg                           >  1:550478/1‑80 (MQ=255)
                                                 gggCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGaaa                        >  2:550493/1‑80 (MQ=255)
                                                  ggCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGaaaa                       <  2:550417/80‑1 (MQ=255)
                                                   gCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAAc                     <  1:550418/81‑1 (MQ=255)
                                                    ccACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAAcg                    <  1:550419/81‑1 (MQ=255)
                                                        gAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGa                 >  2:550496/1‑80 (MQ=255)
                                                        gAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGa                 >  2:550488/1‑80 (MQ=255)
                                                          actgactgCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATAc              <  1:550420/81‑1 (MQ=255)
                                                           ctgactgCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATAcc             <  2:550421/81‑1 (MQ=255)
                                                              actgCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATGCCa            <  2:550425/79‑1 (MQ=255)
                                                              actgCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATACCa            <  1:550423/79‑1 (MQ=255)
                                                              actgCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATACCa            <  2:550422/79‑1 (MQ=255)
                                                              actgCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATACCAAg          <  2:550424/81‑1 (MQ=255)
                                                               ctgCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATACCAAgc         >  1:550486/1‑81 (MQ=255)
                                                               ctgCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATACCAAgc         <  2:550426/81‑1 (MQ=255)
                                                                  cGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATACCAAgcgc       <  2:550427/80‑1 (MQ=255)
                                                                   gATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATACCAAgcgcgc     >  2:550476/1‑81 (MQ=255)
                                                                    aTGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATACCAAGCGCGCt    <  2:550430/81‑1 (MQ=255)
                                                                    aTGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATACCAAGCGCGCt    <  2:550429/81‑1 (MQ=255)
                                                                    aTGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATACCAAGCGCGCt    <  2:550428/81‑1 (MQ=255)
                                                                       cacaGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATACCAAGCGCGCTaa  <  1:550431/80‑1 (MQ=255)
                                                                          |                                                                            
ACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATACCAAGCGCGCTAA  >  NC_000913/3156112‑3156262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A