Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,223,904 |
C→T |
Y424Y (TAC→TAT) |
ebgA → |
evolved beta‑D‑galactosidase subunit alpha |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,223,904 | 0 | C | T | 100.0%
| 149.1
/ NA
| 43 | Y424Y (TAC→TAT) | ebgA | evolved beta‑D‑galactosidase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/21); total (22/21) |
CGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATG > NC_000913/3223826‑3223977
|
cGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGt > 2:563864/1‑81 (MQ=255)
gACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTc < 1:563831/81‑1 (MQ=255)
gACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTc > 2:563881/1‑81 (MQ=255)
gACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTc > 1:563875/1‑81 (MQ=255)
aCGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCg < 2:563833/81‑1 (MQ=255)
aCGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCg < 2:563832/81‑1 (MQ=255)
gTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAg > 1:563869/1‑81 (MQ=255)
aaTCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCa < 2:563834/81‑1 (MQ=255)
cGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTg > 2:563879/1‑81 (MQ=255)
cGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTg > 2:563868/1‑81 (MQ=255)
gCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGt > 2:563876/1‑81 (MQ=255)
gCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGt < 2:563835/81‑1 (MQ=255)
gCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGt > 1:563859/1‑81 (MQ=255)
ggCTTTGCTAATGTCGGCGATACTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGc > 1:563887/1‑81 (MQ=255)
gCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGc > 1:563877/1‑80 (MQ=255)
gCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCatat < 2:563836/80‑1 (MQ=255)
aTGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCAcg > 1:563882/1‑81 (MQ=255)
aTGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCAcg < 2:563837/81‑1 (MQ=255)
tGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCAcgc < 2:563838/81‑1 (MQ=255)
tGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCAcgc > 1:563885/1‑81 (MQ=255)
gTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCAcgcg > 2:563874/1‑81 (MQ=255)
cGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGaa < 2:563840/81‑1 (MQ=255)
cGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGaa < 1:563839/81‑1 (MQ=255)
gATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGaaa < 1:563841/81‑1 (MQ=255)
gATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGaaa < 1:563842/81‑1 (MQ=255)
ttACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCg < 2:563843/80‑1 (MQ=255)
tACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGat > 1:563904/1‑81 (MQ=255)
aCCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGat < 1:563844/80‑1 (MQ=255)
ccGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGatc < 1:563845/80‑1 (MQ=255)
gacgaTCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGatcat > 2:563888/1‑80 (MQ=255)
acgaTCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGatcatca < 2:563846/81‑1 (MQ=255)
cgaTCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGatcatcat > 2:563883/1‑81 (MQ=255)
gaTCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGatcatcatc > 1:563900/1‑81 (MQ=255)
gCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTc < 1:563847/81‑1 (MQ=255)
gCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTc > 2:563906/1‑81 (MQ=255)
aGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGc > 2:563884/1‑81 (MQ=255)
aGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGc > 2:563895/1‑81 (MQ=255)
gTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCt < 2:563848/81‑1 (MQ=255)
gggAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTgg > 2:563886/1‑81 (MQ=255)
aaaaaGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCa < 2:563849/81‑1 (MQ=255)
aaaaGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCaa < 1:563850/81‑1 (MQ=255)
aaaGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAAt < 2:563851/81‑1 (MQ=255)
aGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATg > 2:563891/1‑80 (MQ=255)
|
CGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATG > NC_000913/3223826‑3223977
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
