Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,223,904 |
C→T |
Y424Y (TAC→TAT) |
ebgA → |
evolved beta‑D‑galactosidase subunit alpha |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,223,904 | 0 | C | T | 100.0%
| 133.1
/ NA
| 37 | Y424Y (TAC→TAT) | ebgA | evolved beta‑D‑galactosidase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/22); total (15/22) |
GACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATG > NC_000913/3223827‑3223977
|
gACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTc > 1:606787/1‑81 (MQ=255)
gACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTc > 1:606775/1‑81 (MQ=255)
aCGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCg < 1:606723/81‑1 (MQ=255)
gTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAg > 1:606796/1‑81 (MQ=255)
tCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAgc < 2:606724/81‑1 (MQ=255)
cGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAgcg > 2:606770/1‑81 (MQ=255)
gAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAgcgc < 1:641232/81‑1 (MQ=255)
aaTCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCa < 1:606725/81‑1 (MQ=255)
tCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCAtt < 1:606726/81‑1 (MQ=255)
cGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCAtcg > 2:606793/1‑79 (MQ=255)
gCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGt < 1:606727/81‑1 (MQ=255)
aCGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTc > 2:606777/1‑81 (MQ=255)
aCGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTc < 1:606728/81‑1 (MQ=255)
ggCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGc > 2:606772/1‑81 (MQ=255)
gCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGcc < 1:606729/81‑1 (MQ=255)
tGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCat < 2:606730/79‑1 (MQ=255)
cTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATc < 2:606731/80‑1 (MQ=255)
tAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCa > 2:606792/1‑81 (MQ=255)
aaTGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCAc > 1:606804/1‑81 (MQ=255)
aTGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCAc < 1:606732/80‑1 (MQ=255)
tGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCAcgc < 2:606733/81‑1 (MQ=255)
tCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCAcgcg > 1:606788/1‑80 (MQ=255)
cGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGa > 1:606774/1‑80 (MQ=255)
tatTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGaaaaa < 1:606734/81‑1 (MQ=255)
ttAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAAc < 1:606735/80‑1 (MQ=255)
gCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCAt < 2:606736/80‑1 (MQ=255)
ccGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATcc < 1:606737/81‑1 (MQ=255)
gacgaTCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGatcatc < 1:606738/81‑1 (MQ=255)
gacgaTCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGatcat > 1:606799/1‑80 (MQ=255)
gacgaTCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGatcat > 1:606791/1‑80 (MQ=255)
gCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTc < 2:606739/81‑1 (MQ=255)
gCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTc > 2:606790/1‑81 (MQ=255)
gTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCt > 1:606789/1‑81 (MQ=255)
gAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGc < 2:606740/81‑1 (MQ=255)
aaaGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAAt < 1:606742/81‑1 (MQ=255)
aaaGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAAt < 1:606741/81‑1 (MQ=255)
aaGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATg < 2:606743/81‑1 (MQ=255)
|
GACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATG > NC_000913/3223827‑3223977
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
