Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,225,566 |
C→T |
S978S (AGC→AGT) |
ebgA → |
evolved beta‑D‑galactosidase subunit alpha |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,225,566 | 0 | C | T | 100.0%
| 112.4
/ NA
| 31 | S978S (AGC→AGT) | ebgA | evolved beta‑D‑galactosidase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/15); total (16/15) |
GAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCGGAGA > NC_000913/3225489‑3225643
|
gAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAg > 1:607236/1‑81 (MQ=255)
cAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCt < 2:607190/80‑1 (MQ=255)
aGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTgg < 1:607191/81‑1 (MQ=255)
gcAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACt > 1:607245/1‑81 (MQ=255)
aGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTcc < 1:607192/81‑1 (MQ=255)
tgatgaCATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTg < 2:607193/81‑1 (MQ=255)
aTCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTc > 2:607249/1‑81 (MQ=255)
aTCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTc < 1:607194/81‑1 (MQ=255)
aTCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTc > 2:607237/1‑81 (MQ=255)
cACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTg > 1:607232/1‑81 (MQ=255)
aaCCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCg < 2:607195/80‑1 (MQ=255)
aCCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGt > 1:607246/1‑80 (MQ=255)
cGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACtt > 1:607254/1‑81 (MQ=255)
cGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACtt > 2:607239/1‑81 (MQ=255)
gATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTc < 1:607196/81‑1 (MQ=255)
aTCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACtt > 1:607251/1‑79 (MQ=255)
aTCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCa < 1:607197/81‑1 (MQ=255)
cACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGc > 1:607240/1‑81 (MQ=255)
aCCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCt < 1:607198/81‑1 (MQ=255)
aGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACg > 1:607247/1‑81 (MQ=255)
aGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACg > 2:607244/1‑81 (MQ=255)
ctTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCtt < 1:607199/80‑1 (MQ=255)
ttGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTa > 1:607250/1‑81 (MQ=255)
ttGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTa > 2:607235/1‑81 (MQ=255)
gCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTtgctgc < 1:607200/81‑1 (MQ=255)
cTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGcc > 1:607228/1‑81 (MQ=255)
cAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCgg < 1:607201/80‑1 (MQ=255)
ccTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTg > 1:607252/1‑81 (MQ=255)
gggCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCgg < 1:607202/81‑1 (MQ=255)
gCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCGgag < 2:607203/81‑1 (MQ=255)
cAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCGgaga < 1:607204/81‑1 (MQ=255)
|
GAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCGGAGA > NC_000913/3225489‑3225643
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
