Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,225,566 |
C→T |
S978S (AGC→AGT) |
ebgA → |
evolved beta‑D‑galactosidase subunit alpha |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,225,566 | 0 | C | T | 100.0%
| 104.2
/ NA
| 31 | S978S (AGC→AGT) | ebgA | evolved beta‑D‑galactosidase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (13/18); total (13/18) |
CTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCGG > NC_000913/3225492‑3225640
|
cTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTg < 1:564271/81‑1 (MQ=255)
cTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTg > 2:564330/1‑81 (MQ=255)
cAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCt < 2:564272/80‑1 (MQ=255)
aGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTgg < 1:564273/81‑1 (MQ=255)
aGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTgg < 2:564274/81‑1 (MQ=255)
cAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTc > 1:564312/1‑81 (MQ=255)
gTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCt > 2:564313/1‑81 (MQ=255)
tgatgaCATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTg < 1:564275/81‑1 (MQ=255)
tgatgaCATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTg < 2:564276/81‑1 (MQ=255)
cATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGt > 1:564329/1‑81 (MQ=255)
tCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCt < 2:564277/81‑1 (MQ=255)
tCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCt < 1:564278/81‑1 (MQ=255)
cACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTg > 2:564323/1‑81 (MQ=255)
aCCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTgg > 2:564320/1‑81 (MQ=255)
gATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTc < 2:564280/81‑1 (MQ=255)
gATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTc < 1:564279/81‑1 (MQ=255)
cAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTAc < 1:564282/81‑1 (MQ=255)
cAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTAc > 1:564342/1‑81 (MQ=255)
cAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTAc < 1:564281/81‑1 (MQ=255)
aGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACg > 2:564343/1‑81 (MQ=255)
ctTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCttt < 1:564283/81‑1 (MQ=255)
ctTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCt < 1:564284/79‑1 (MQ=255)
cGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTtgct > 2:564333/1‑81 (MQ=255)
ggCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTtgctg < 2:564285/81‑1 (MQ=255)
ggCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTtgctg < 2:564286/81‑1 (MQ=255)
gCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTtgctgc > 2:564336/1‑81 (MQ=255)
cTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGcc < 2:564287/81‑1 (MQ=255)
cTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGAGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTgg > 1:564332/1‑81 (MQ=255)
ggggCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCg > 1:564331/1‑81 (MQ=255)
ggggCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCg > 2:564341/1‑81 (MQ=255)
gggCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCgg < 1:564288/81‑1 (MQ=255)
|
CTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCGG > NC_000913/3225492‑3225640
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
