Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,297,253 |
C→T |
A296T (GCT→ACT) |
yraQ ← |
permease family protein YraQ |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,297,253 | 0 | C | T | 100.0%
| 141.4
/ NA
| 40 | A296T (GCT→ACT) | yraQ | permease family protein YraQ |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (18/22); total (18/22) |
GGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTG > NC_000913/3297178‑3297330
|
ggAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTc < 2:578028/81‑1 (MQ=255)
ggAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTc > 1:578073/1‑81 (MQ=255)
aaCGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTccc < 1:578029/81‑1 (MQ=255)
aCGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCa > 2:578068/1‑81 (MQ=255)
cTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATAc > 1:578079/1‑81 (MQ=255)
tttGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATAcc > 1:578075/1‑81 (MQ=255)
cgcAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTTGTTCCCATACCTGcc < 2:578030/81‑1 (MQ=255)
cgcAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGc > 2:578096/1‑80 (MQ=255)
gcagcaTAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGc > 2:578074/1‑79 (MQ=255)
cagcaTAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAg > 2:578078/1‑81 (MQ=255)
agcaTAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGc < 2:578031/81‑1 (MQ=255)
caTAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGcat < 2:578032/81‑1 (MQ=255)
aTAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGcat > 1:578071/1‑80 (MQ=255)
aaTCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGcatcat > 2:578087/1‑81 (MQ=255)
aTCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGcatcat < 2:578035/80‑1 (MQ=255)
aTCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCatcatc < 2:578034/81‑1 (MQ=255)
aTCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCatcatc < 2:578033/81‑1 (MQ=255)
tCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCg < 2:578036/81‑1 (MQ=255)
aGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGtt < 2:578037/81‑1 (MQ=255)
gTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGttt > 1:578084/1‑81 (MQ=255)
gACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTg > 1:578080/1‑80 (MQ=255)
aCGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTa < 2:578038/81‑1 (MQ=255)
aCTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCgg < 2:578039/81‑1 (MQ=255)
gCCGGGAGCGTCATTAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTct > 2:578086/1‑81 (MQ=255)
gggAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTctgct < 1:578040/81‑1 (MQ=255)
ggggCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTctgctg < 1:578042/78‑1 (MQ=255)
ggAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTctgctg > 2:578066/1‑81 (MQ=255)
ggAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTctgctg < 1:578041/81‑1 (MQ=255)
aGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGcc > 1:578095/1‑81 (MQ=255)
cGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGt < 1:578043/81‑1 (MQ=255)
gTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTg < 1:578044/81‑1 (MQ=255)
atcaACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTggg < 1:578045/80‑1 (MQ=255)
aacaaTGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAAt > 1:578085/1‑80 (MQ=255)
caaTGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGa > 2:578098/1‑80 (MQ=255)
aaTGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGac > 2:578089/1‑80 (MQ=255)
ccAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGacaaac < 2:578046/80‑1 (MQ=255)
gcgcCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGc < 2:578047/81‑1 (MQ=255)
cgcCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCa > 1:578090/1‑81 (MQ=255)
gcCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCAt < 2:578048/81‑1 (MQ=255)
gAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTg < 2:578049/81‑1 (MQ=255)
|
GGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTG > NC_000913/3297178‑3297330
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
