Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,471,692 |
G→T |
A608E (GCG→GAG) |
fusA ← |
elongation factor G |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,471,692 | 0 | G | T | 100.0%
| 94.8
/ NA
| 28 | A608E (GCG→GAG) | fusA | elongation factor G |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (17/11); total (17/11) |
ACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCA > NC_000913/3471616‑3471763
|
aCCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCttt > 2:613510/1‑81 (MQ=255)
cGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCt > 2:613512/1‑81 (MQ=255)
tAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTaa < 1:613471/81‑1 (MQ=255)
aCGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAg > 1:613508/1‑81 (MQ=255)
cGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGc < 2:613472/81‑1 (MQ=255)
cGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGc < 2:613473/81‑1 (MQ=255)
gTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCCCAAGCAGAACTGGTTTCTCTTTCTTAAAGcc > 2:613499/1‑81 (MQ=255)
cACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCctt > 2:613503/1‑81 (MQ=255)
aCCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCcttc > 1:613509/1‑81 (MQ=255)
ggTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCcttctt > 2:613511/1‑80 (MQ=255)
ttCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAg > 1:613507/1‑81 (MQ=255)
tCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGa > 2:613524/1‑79 (MQ=255)
ccGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGAt < 2:613474/79‑1 (MQ=255)
aGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAagc < 2:613475/81‑1 (MQ=255)
cTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGt < 1:613476/80‑1 (MQ=255)
cAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTa < 2:613477/80‑1 (MQ=255)
aaCCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTaaa > 2:613521/1‑81 (MQ=255)
aCCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAAc > 2:613495/1‑81 (MQ=255)
aTCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGtt > 2:613520/1‑79 (MQ=255)
aTCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTc < 2:613478/80‑1 (MQ=255)
ggCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCagag > 1:613528/1‑81 (MQ=255)
ggCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCagag > 1:613526/1‑81 (MQ=255)
gCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAgagg < 2:613479/81‑1 (MQ=255)
aaGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGt < 1:613480/80‑1 (MQ=255)
aaGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTc < 2:613481/81‑1 (MQ=255)
aGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTc > 1:613514/1‑80 (MQ=255)
aGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACg > 2:613519/1‑81 (MQ=255)
aCTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCa > 1:613517/1‑81 (MQ=255)
|
ACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCA > NC_000913/3471616‑3471763
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
