Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,471,692 |
G→T |
A608E (GCG→GAG) |
fusA ← |
elongation factor G |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,471,692 | 0 | G | T | 100.0%
| 118.6
/ NA
| 33 | A608E (GCG→GAG) | fusA | elongation factor G |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (16/17); total (16/17) |
CACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTC > NC_000913/3471615‑3471762
|
cACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCtt > 1:662122/1‑81 (MQ=255)
cGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCt > 2:662136/1‑81 (MQ=255)
gATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCtctttctt < 1:662079/81‑1 (MQ=255)
aTAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTa < 2:662080/81‑1 (MQ=255)
tAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTaa < 1:662081/81‑1 (MQ=255)
tAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTaa < 2:662082/81‑1 (MQ=255)
gTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGcc > 1:662132/1‑81 (MQ=255)
tCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCct < 1:662083/81‑1 (MQ=255)
ggTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCttt < 1:662084/81‑1 (MQ=255)
gtgtTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTa < 1:662085/81‑1 (MQ=255)
gtTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTa > 2:662143/1‑79 (MQ=255)
ccGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATa < 2:662086/80‑1 (MQ=255)
ggAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAg > 1:662146/1‑79 (MQ=255)
ggAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAg > 1:662133/1‑79 (MQ=255)
gAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAag < 2:662087/81‑1 (MQ=255)
aGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAag > 1:662138/1‑80 (MQ=255)
tACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAg > 1:662135/1‑81 (MQ=255)
cTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGtt < 1:662088/81‑1 (MQ=255)
cAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGttt < 1:662089/79‑1 (MQ=255)
cAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGttt < 2:662090/79‑1 (MQ=255)
cAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTa > 2:662134/1‑80 (MQ=255)
aCCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAAc < 2:662091/81‑1 (MQ=255)
cTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGc > 1:662137/1‑81 (MQ=255)
cATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGcc < 2:662092/79‑1 (MQ=255)
gATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGtt > 2:662140/1‑80 (MQ=255)
gATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTc < 1:662093/81‑1 (MQ=255)
cGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCa > 2:662151/1‑79 (MQ=255)
ggCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCagag > 1:662145/1‑81 (MQ=255)
gCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTCTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCagag > 2:662142/1‑80 (MQ=255)
tCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAgaggag > 2:662147/1‑81 (MQ=255)
cAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAAc < 2:662094/81‑1 (MQ=255)
aGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACg > 2:662144/1‑81 (MQ=255)
gAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGt < 1:662095/81‑1 (MQ=255)
aaCTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTc < 1:662096/81‑1 (MQ=255)
|
CACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTC > NC_000913/3471615‑3471762
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
