Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,547,765 |
G→T |
E403* (GAG→TAG) |
gntT → |
high‑affinity gluconate transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,547,765 | 0 | G | T | 100.0%
| 148.3
/ NA
| 43 | E403* (GAG→TAG) | gntT | high‑affinity gluconate transporter |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (28/15); total (28/15) |
AGCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCTG > NC_000913/3547688‑3547842
|
aGCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAatag > 1:630155/1‑80 (MQ=255)
cTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTAc > 2:630158/1‑81 (MQ=255)
tGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACt > 2:630153/1‑81 (MQ=255)
gATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACtt > 2:630142/1‑81 (MQ=255)
aTTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCt > 2:630157/1‑80 (MQ=255)
ttGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGa < 2:630110/81‑1 (MQ=255)
gCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGAc > 1:630164/1‑80 (MQ=255)
cGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACt < 1:630112/80‑1 (MQ=255)
cGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACt < 1:630111/80‑1 (MQ=255)
ggttggttCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTAt < 2:630113/81‑1 (MQ=255)
gttggttCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATc > 2:630162/1‑81 (MQ=255)
tggttCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCgg > 1:630161/1‑81 (MQ=255)
tggttCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCgg > 1:630181/1‑81 (MQ=255)
ggttCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGc > 2:630154/1‑81 (MQ=255)
gttCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGc > 1:630170/1‑80 (MQ=255)
gtgtGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCa < 2:630114/81‑1 (MQ=255)
aTTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATc < 1:630115/80‑1 (MQ=255)
tctctcATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTgg < 1:630116/80‑1 (MQ=255)
ctctcATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTc > 1:630166/1‑81 (MQ=255)
tcATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTc < 1:630117/78‑1 (MQ=255)
cATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGATTATCGGCGAGACCATCAAATCCTGGTCGATg > 2:630185/1‑81 (MQ=255)
cATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATg > 2:630163/1‑81 (MQ=255)
cATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATg > 2:630171/1‑81 (MQ=255)
aTGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGc < 2:630118/81‑1 (MQ=255)
tgtgAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCt < 1:630119/81‑1 (MQ=255)
tgAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTg > 2:630208/1‑80 (MQ=255)
gAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTgg > 2:630168/1‑80 (MQ=255)
cGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAAc > 2:630159/1‑81 (MQ=255)
tCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCAt < 2:630120/81‑1 (MQ=255)
cGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTa > 2:630186/1‑81 (MQ=255)
gggCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTAt > 2:630167/1‑81 (MQ=255)
cTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCAGGTCGATGCTGGAAACCATTAtctt < 1:630121/81‑2 (MQ=255)
cTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCgg < 2:630122/80‑1 (MQ=255)
cTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGgt > 1:630172/1‑81 (MQ=255)
ggCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGgtgt > 1:630175/1‑81 (MQ=255)
gCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGgtg < 1:630124/79‑1 (MQ=255)
gCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGgtg < 2:630123/79‑1 (MQ=255)
gCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGtgtg > 2:630183/1‑81 (MQ=255)
gTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCgg > 2:630160/1‑81 (MQ=255)
ttCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGt > 2:630176/1‑81 (MQ=255)
ttCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGt > 2:630178/1‑81 (MQ=255)
cAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCt > 2:630192/1‑81 (MQ=255)
aaaTAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCTg > 1:630165/1‑81 (MQ=255)
aaaTAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCTg > 2:630187/1‑81 (MQ=255)
|
AGCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCTG > NC_000913/3547688‑3547842
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
