Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,547,765 |
G→T |
E403* (GAG→TAG) |
gntT → |
high‑affinity gluconate transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,547,765 | 0 | G | T | 100.0%
| 159.5
/ NA
| 44 | E403* (GAG→TAG) | gntT | high‑affinity gluconate transporter |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (30/14); total (30/14) |
GCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCT > NC_000913/3547689‑3547841
|
gCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTa > 2:680589/1‑81 (MQ=255)
gCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTa > 2:680601/1‑81 (MQ=255)
cTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTAc > 2:680587/1‑81 (MQ=255)
tGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACt > 1:680583/1‑81 (MQ=255)
gATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACtt > 1:680614/1‑81 (MQ=255)
aTGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACtt > 1:680577/1‑80 (MQ=255)
gTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTaa < 1:680536/80‑1 (MQ=255)
ttATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAAcc < 1:680537/81‑1 (MQ=255)
ttATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAAcc > 1:680610/1‑81 (MQ=255)
tATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCt > 2:680609/1‑81 (MQ=255)
ttGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGa > 2:680564/1‑81 (MQ=255)
gCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGAc > 1:680538/1‑80 (MQ=255)
cGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACt < 1:680539/80‑1 (MQ=255)
cGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTa > 2:680620/1‑81 (MQ=255)
ggttggttCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTAt > 1:680613/1‑81 (MQ=255)
ggttCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGc > 2:680584/1‑81 (MQ=255)
ggttCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGc > 2:680582/1‑81 (MQ=255)
ggTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGAc > 1:680594/1‑80 (MQ=255)
gTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCa < 2:680540/81‑1 (MQ=255)
gtgtGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATc > 2:680604/1‑80 (MQ=255)
gtgATTTTATCTAATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCaaa > 2:680588/1‑81 (MQ=255)
tttCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTg > 1:680585/1‑81 (MQ=255)
tctctcATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGt > 1:680595/1‑81 (MQ=255)
ctcATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGa > 2:680599/1‑81 (MQ=255)
ctcATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGa > 2:680631/1‑81 (MQ=255)
tcATGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGAt > 2:680622/1‑81 (MQ=255)
aTGTGAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGc > 2:680611/1‑81 (MQ=255)
tgtgAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCt < 1:680541/81‑1 (MQ=255)
gtgAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTg > 2:680615/1‑81 (MQ=255)
tgAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTgg < 1:680543/81‑1 (MQ=255)
tgAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTg < 2:680542/80‑1 (MQ=255)
gAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTgg > 1:680621/1‑80 (MQ=255)
gAACGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTgg > 1:680597/1‑80 (MQ=255)
aCGATCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGaaa > 2:680602/1‑81 (MQ=255)
aTCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCa < 1:680544/81‑1 (MQ=255)
tCCGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCAt > 2:680623/1‑81 (MQ=255)
cGGGCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCAtt < 2:680545/80‑1 (MQ=255)
gggCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTAt < 1:680546/81‑1 (MQ=255)
ggCTTCTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTAtc > 2:680607/1‑81 (MQ=255)
cTGGCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGgt > 2:680612/1‑81 (MQ=255)
gCTGTTCAAATAGTACTTTAACCTGACTGTCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGgt < 2:680547/78‑1 (MQ=255)
gCTGTTCAAATAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGgt < 1:680548/78‑1 (MQ=255)
aaaTAGTACTTTAACCTGACTATCGGCGAGACCGTCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCt < 1:680550/80‑1 (MQ=255)
aaaTAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCt < 2:680549/80‑1 (MQ=255)
|
GCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCT > NC_000913/3547689‑3547841
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
