Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,589,355 |
C→T |
V225M (GTG→ATG) |
ugpE ← |
sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpE |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,589,355 | 0 | C | T | 100.0%
| 156.6
/ NA
| 44 | V225M (GTG→ATG) | ugpE | sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpE |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (24/20); total (24/20) |
TCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGAT > NC_000913/3589277‑3589432
|
tCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCatat < 1:638828/81‑1 (MQ=255)
cTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCgg > 2:638867/1‑81 (MQ=255)
aGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGta < 1:638829/80‑1 (MQ=255)
gTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGtaat < 1:638831/81‑1 (MQ=255)
gTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGtaat < 1:638830/81‑1 (MQ=255)
tCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGtaataa > 2:638901/1‑81 (MQ=255)
ttCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATcaa < 2:638832/81‑1 (MQ=255)
ccGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATcaac < 2:638833/80‑1 (MQ=255)
gtggtgCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCaacaac > 1:638872/1‑81 (MQ=255)
gtggtgCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCaacaac < 2:638834/81‑1 (MQ=255)
ggtgCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACgg > 1:638870/1‑81 (MQ=255)
gCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCca > 2:638886/1‑81 (MQ=255)
ccTTCGCCTGTAGCGATCATCCCTTTGATCCCCGCCACGGTGGTGCCGAGATCCATATCGGTATTAATCAACAACGGCcac < 2:638835/81‑1 (MQ=255)
ccTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATAc > 2:638885/1‑81 (MQ=255)
cTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACt < 2:638836/81‑1 (MQ=255)
tAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTg < 2:638838/79‑1 (MQ=255)
tAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTg < 2:638837/79‑1 (MQ=255)
tAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGa < 1:638839/80‑1 (MQ=255)
gATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTc > 1:638877/1‑79 (MQ=255)
gATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTc > 1:638904/1‑79 (MQ=255)
gATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCa < 1:638840/81‑1 (MQ=255)
atcatcCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAg > 1:638895/1‑81 (MQ=255)
atcatcCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAg < 1:638841/81‑1 (MQ=255)
cccTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCg > 1:638893/1‑79 (MQ=255)
cccTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCg > 2:638897/1‑79 (MQ=255)
ccTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAg < 1:638843/81‑1 (MQ=255)
ccTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAg < 2:638842/81‑1 (MQ=255)
gATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATaa < 1:638844/80‑1 (MQ=255)
cccTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGt > 2:638878/1‑81 (MQ=255)
ccTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGt > 1:638907/1‑80 (MQ=255)
gCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGAt > 1:638879/1‑80 (MQ=255)
gCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGAt > 1:638874/1‑80 (MQ=255)
cACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATcac < 2:638845/81‑1 (MQ=255)
aCGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATcac > 1:638892/1‑80 (MQ=255)
ggtggtGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACaaa < 1:638846/81‑1 (MQ=255)
ggtggtGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACaaa > 1:638882/1‑81 (MQ=255)
ggtggtGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACaaa > 2:638891/1‑81 (MQ=255)
tggtgCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACaaa > 2:638921/1‑79 (MQ=255)
gtgCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAgc > 1:638883/1‑81 (MQ=255)
gtgCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAgc > 2:638896/1‑81 (MQ=255)
gCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAgcgc > 1:638890/1‑81 (MQ=255)
agaTCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGcgccgc > 1:638881/1‑80 (MQ=255)
agaTCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCgccgcc < 1:638848/81‑1 (MQ=255)
agaTCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCgccgcc < 2:638847/81‑1 (MQ=255)
ccATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGAt > 1:638906/1‑81 (MQ=255)
|
TCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGAT > NC_000913/3589277‑3589432
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
