Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,601,119 |
C→T |
C323Y (TGC→TAC) |
ftsX ← |
cell division protein FtsX |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,601,119 | 0 | C | T | 100.0%
| 75.3
/ NA
| 23 | C323Y (TGC→TAC) | ftsX | cell division protein FtsX |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (10/13); total (10/13) |
GTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAACCGC > NC_000913/3601048‑3601186
|
gTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGTATTCATCGa < 2:641462/81‑1 (MQ=255)
cGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCAtt < 1:641463/81‑1 (MQ=255)
gTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTg < 2:641464/81‑1 (MQ=255)
cAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTGatat > 2:641484/1‑81 (MQ=255)
aCGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACt > 2:641504/1‑81 (MQ=255)
aCGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACt < 2:641465/81‑1 (MQ=255)
gCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTc > 2:641502/1‑81 (MQ=255)
cTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCg < 1:641466/81‑1 (MQ=255)
tGCCACCCAGCCAATCATCGAGCGTACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGt > 1:641494/1‑81 (MQ=255)
tGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGt > 2:641516/1‑81 (MQ=255)
caaCCCAGCCAATCATCGAGCATACCAGCAGCAATAGGAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGtt < 1:641467/78‑1 (MQ=255)
ccAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGaa < 2:641468/81‑1 (MQ=255)
ccAATCATCGAGCATACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAAcc < 1:641469/81‑1 (MQ=255)
cGAGCATACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCAc > 1:641499/1‑81 (MQ=255)
cGAGCATACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCAc > 1:641500/1‑81 (MQ=255)
aTACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCg > 2:641508/1‑81 (MQ=255)
tACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCg > 2:641491/1‑80 (MQ=255)
ccAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCaa < 2:641470/81‑1 (MQ=255)
cagcaATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAAccgc > 1:641498/1‑81 (MQ=255)
cagcaATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAAccgc < 1:641474/81‑1 (MQ=255)
cagcaATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAAccgc < 1:641473/81‑1 (MQ=255)
cagcaATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAAccgc < 2:641472/81‑1 (MQ=255)
cagcaATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAAccgc < 1:641471/81‑1 (MQ=255)
|
GTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAACCGC > NC_000913/3601048‑3601186
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
