Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,589,355 |
C→T |
V225M (GTG→ATG) |
ugpE ← |
sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpE |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,589,355 | 0 | C | T | 100.0%
| 81.6
/ NA
| 23 | V225M (GTG→ATG) | ugpE | sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpE |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (10/13); total (10/13) |
TGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCC > NC_000913/3589281‑3589428
|
tGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCgg > 1:690311/1‑80 (MQ=255)
aGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGta < 1:690266/80‑1 (MQ=255)
aGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGt < 1:690267/79‑1 (MQ=255)
aGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGt < 1:690268/79‑1 (MQ=255)
gTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGtaat < 2:690269/81‑1 (MQ=255)
cATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTaataat > 2:690314/1‑80 (MQ=255)
cATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTaataat > 1:690324/1‑80 (MQ=255)
ttCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATcaa > 1:690317/1‑81 (MQ=255)
tggtgCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACg < 1:690270/81‑1 (MQ=255)
gtgCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACgg < 2:690271/80‑1 (MQ=255)
ccTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCcac < 1:690272/81‑1 (MQ=255)
cGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACaaa < 1:690273/80‑1 (MQ=255)
tGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACt > 2:690316/1‑80 (MQ=255)
cGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTc > 1:690313/1‑80 (MQ=255)
cTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGa < 2:690274/81‑1 (MQ=255)
ttGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGAt > 2:690320/1‑80 (MQ=255)
aTCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAg < 1:690275/81‑1 (MQ=255)
cTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGa > 2:690331/1‑81 (MQ=255)
ccACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGAt < 1:690276/79‑1 (MQ=255)
ggtggtGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACaaa > 2:690323/1‑81 (MQ=255)
ccGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGcgcc < 1:690277/81‑1 (MQ=255)
gagaTCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGcgccgc > 1:690340/1‑81 (MQ=255)
agaTCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCgccgcc < 1:690278/81‑1 (MQ=255)
|
TGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCC > NC_000913/3589281‑3589428
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
