Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,608,490 |
C→T |
A680A (GCC→GCT) |
zntA → |
Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,608,490 | 0 | C | T | 100.0%
| 97.8
/ NA
| 29 | A680A (GCC→GCT) | zntA | Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (19/10); total (19/10) |
GCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGG > NC_000913/3608416‑3608567
|
gccgACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATc > 1:643202/1‑81 (MQ=255)
gccgACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATc < 1:643163/81‑1 (MQ=255)
gACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGc > 2:643210/1‑81 (MQ=255)
aCGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGc > 2:643199/1‑80 (MQ=255)
caTTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACa > 1:643201/1‑81 (MQ=255)
aCCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACt < 1:643164/81‑1 (MQ=255)
cccATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTa > 2:643205/1‑81 (MQ=255)
cccATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTa > 1:643208/1‑81 (MQ=255)
aaCCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTgcg > 2:643200/1‑81 (MQ=255)
ccaccTGCGCGGCCTGGTGCAAATGATTGAGCTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCt < 1:643165/81‑1 (MQ=255)
caccTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTg > 1:643193/1‑81 (MQ=255)
accTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTgg > 1:643203/1‑81 (MQ=255)
ggCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGa > 1:643213/1‑79 (MQ=255)
ggCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGa > 2:643206/1‑79 (MQ=255)
ggTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGAt > 1:643240/1‑81 (MQ=255)
cAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTc < 2:643166/81‑1 (MQ=255)
aTGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTc < 1:643167/81‑1 (MQ=255)
tGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCg > 1:643216/1‑81 (MQ=255)
tGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCg < 1:643168/81‑1 (MQ=255)
aTTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGt > 1:643212/1‑80 (MQ=255)
aTTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTc < 1:643169/81‑1 (MQ=255)
gAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTcac > 2:643224/1‑80 (MQ=255)
aCTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTcaccac < 1:643170/81‑1 (MQ=255)
gCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTg > 1:643214/1‑81 (MQ=255)
cACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGt > 1:643215/1‑81 (MQ=255)
aCGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGtt < 2:643171/81‑1 (MQ=255)
cACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGAcc > 2:643217/1‑81 (MQ=255)
aCGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGAcc < 1:643172/80‑1 (MQ=255)
cGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCgg > 1:643218/1‑81 (MQ=255)
|
GCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGG > NC_000913/3608416‑3608567
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
