Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,665,562 |
G→A |
L83L (CTC→CTT) |
gadX ← |
DNA‑binding transcriptional dual regulator GadX |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,665,562 | 0 | G | A | 100.0%
| 61.2
/ NA
| 19 | L83L (CTC→CTT) | gadX | DNA‑binding transcriptional dual regulator GadX |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (4/15); total (4/15) |
CATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGT > NC_000913/3665486‑3665639
|
cATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACAAGTa < 2:654234/81‑1 (MQ=255)
ctCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACAAGTAATGAAGAAACt > 2:654268/2‑81 (MQ=255)
cTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACAAGTAATGAAGAAACtt < 1:654235/80‑1 (MQ=255)
ttATTCTGCGTTATTTTCGCAACTTCCTGAAAAACAGGAATTTCTCTACTACAGTGATGAACAAGTAATGAAGAAACtttt > 2:866883/1‑81 (MQ=255)
aTTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACAAGTAATGAAGAAACttttt < 1:654237/80‑1 (MQ=255)
aTTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACAAGTAATGAAGAAACttttt < 1:654239/80‑1 (MQ=255)
aTTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACAAGTAATGAAGAAACtttt < 1:654238/79‑1 (MQ=255)
aTTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACAAGTAATGAAGAAACttt < 2:654236/78‑1 (MQ=255)
aaaaaCAGGAATATCTCTACTACAGTGATGAACAAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGcctcctc < 2:654240/81‑1 (MQ=255)
aaaCAGGAATATCTCTACTACAGTGATGAACAAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGcctcctcct > 1:654263/1‑81 (MQ=255)
aaCAGGAATATCTCTACTACAGTGATGAACAAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCtt < 2:654241/81‑1 (MQ=255)
aCAGGAATATCTCTACTACAGTGATGAACAAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTg < 2:654242/81‑1 (MQ=255)
atatCTCTACTACAGTGATGAACAAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATc < 2:654243/81‑1 (MQ=255)
tactacAGTGATGAACAAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGgaga > 1:654266/1‑80 (MQ=255)
tactacAGTGATGAACAAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGACTCCTCCTTGAGCATCGGgag < 1:654244/79‑1 (MQ=255)
ctacAGTGATGAACAAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAaat < 1:654245/81‑1 (MQ=255)
ctacAGTGATGAACAAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAaat < 1:654246/81‑1 (MQ=255)
tgaACAAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGc < 2:654247/81‑1 (MQ=255)
aaCAAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCgt < 1:654248/81‑1 (MQ=255)
|
CATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGT > NC_000913/3665486‑3665639
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
