Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,665,991 |
A→G |
intergenic (‑181/+189) |
gadX ← / ← gadA |
DNA‑binding transcriptional dual regulator GadX/glutamate decarboxylase A |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,665,991 | 0 | A | G | 100.0%
| 60.5
/ NA
| 18 | intergenic (‑181/+189) | gadX/gadA | DNA‑binding transcriptional dual regulator GadX/glutamate decarboxylase A |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (4/14); total (4/14) |
AGGATGATAATGTGTGATGTTCTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATAT > NC_000913/3665914‑3666067
|
aGGATGATAATGTGTGATGTTCTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAGTTa < 2:707933/81‑1 (MQ=255)
atAATGTGTGATGTTCTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAGTTAATAGt < 1:707934/80‑1 (MQ=255)
tAATGTGTGATGTTCTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAGTTAATAGTaa < 2:707935/81‑1 (MQ=255)
tgtgATGTTCTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAGTTAATAGTAAGCaa < 2:707936/80‑1 (MQ=255)
cTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAGTTAATAGTAAGCAAATAATCCCt < 2:707938/80‑1 (MQ=255)
cTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAGTTAATAGTAAGCAAATAATCCCt < 2:707937/80‑1 (MQ=255)
aGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAGTTAATAGTAAGCAAATAATCCCTTTTCGTGa > 1:707955/1‑80 (MQ=255)
gATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAGTTAATAGTAAGCAAATAATCCCTTTTCGTGACAt < 2:707939/81‑1 (MQ=255)
gATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAGTTAATAGTAAGCAAATAATCCCTTTTCGTGACAt > 1:707956/1‑81 (MQ=255)
aCTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAGTTAATAGTAAGCAAATAATCCCTTTTCGTGACATTaaa < 1:707940/81‑1 (MQ=255)
gATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAGTTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTa < 1:707941/81‑1 (MQ=255)
tataATACAAAAACGTGTTTAAGAACACAGGGAAGTTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATc < 2:707942/80‑1 (MQ=255)
cAAAAACGTGTTTAAGAACACAGGGAAGTTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACAt < 2:707943/80‑1 (MQ=255)
aaaaaCGTGTTTAAGAACACAGGGAAGTTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACAtt > 2:707957/1‑80 (MQ=255)
aaCGTGTTTAAGAACACAGGGAAGTTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTtaa > 1:707959/1‑80 (MQ=255)
gAACACAGGGAAGTTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCata < 2:707944/81‑1 (MQ=255)
gggAAGTTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACata < 2:707945/81‑1 (MQ=255)
ggAAGTTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACatat < 1:707946/81‑1 (MQ=255)
|
AGGATGATAATGTGTGATGTTCTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATAT > NC_000913/3665914‑3666067
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
