Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,738,556 |
A→G |
H238R (CAT→CGT) |
purR → |
DNA‑binding transcriptional repressor PurR |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,738,556 | 0 | A | G | 100.0%
| 88.3
/ NA
| 26 | H238R (CAT→CGT) | purR | DNA‑binding transcriptional repressor PurR |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (12/14); total (12/14) |
CGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGC > NC_000913/1738478‑1738633
|
cGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCGTc < 2:321502/81‑1 (MQ=255)
aGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCGTCGccc > 1:321545/1‑80 (MQ=255)
gCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTa > 1:321558/1‑81 (MQ=255)
gCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTa > 1:321544/1‑81 (MQ=255)
cTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTAc < 1:321503/81‑1 (MQ=255)
ggATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTACTg > 1:321562/1‑81 (MQ=255)
gATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTACTg < 2:321504/80‑1 (MQ=255)
ggTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCtgt < 1:321505/81‑1 (MQ=255)
tGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGc < 1:321506/79‑1 (MQ=255)
tGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCg < 1:321507/80‑1 (MQ=255)
aaCCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGat < 2:321508/80‑1 (MQ=255)
aaTCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATgg > 2:321552/1‑81 (MQ=255)
aaTCCGGTTATCGCGACATGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATgg < 2:321509/81‑1 (MQ=255)
cgcgCCATGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCa > 1:486749/1‑81 (MQ=255)
cATGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTtg < 1:321510/81‑1 (MQ=255)
cATGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTtg > 2:321564/1‑81 (MQ=255)
aTGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTtgt < 2:321511/81‑1 (MQ=255)
aTGCAGCAAATCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTtgt < 2:321512/81‑1 (MQ=255)
cagcaAATCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGtgc < 2:321513/80‑1 (MQ=255)
agcaAATCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGtgctg > 2:321555/1‑81 (MQ=255)
caAATCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTgctgct < 2:321514/81‑1 (MQ=255)
aaaTCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTgctgct > 1:321571/1‑80 (MQ=255)
aaaTCCTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTgctgct > 1:321557/1‑80 (MQ=255)
cTGTCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCtgatga > 2:321560/1‑80 (MQ=255)
tCGCAGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGaaa < 1:321515/79‑1 (MQ=255)
aGCCGCGTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGcc > 1:321565/1‑81 (MQ=255)
cgcgTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTgc < 2:321516/81‑1 (MQ=255)
cgcgTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTgc > 2:321569/1‑81 (MQ=255)
|
CGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGC > NC_000913/1738478‑1738633
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
