Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,539,484 |
T→A |
N34Y (AAT→TAT) |
cysM ← |
cysteine synthase B |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,539,484 | 0 | T | A | 100.0%
| 104.2
/ NA
| 29 | N34Y (AAT→TAT) | cysM | cysteine synthase B |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (11/18); total (11/18) |
TTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGTTTTAACCACACTTCACTGCCGTTATCCGGCCCCATTCGCTGCAACTTCACCAGAGGCGTA > NC_000913/2539407‑2539554
|
tttAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGcc < 1:462458/81‑1 (MQ=255)
aaTTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCtt < 1:462459/80‑1 (MQ=255)
aaTTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTc > 2:462502/1‑81 (MQ=255)
tCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAg < 1:462461/80‑1 (MQ=255)
tCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAg < 2:462460/80‑1 (MQ=255)
ccccGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGtt > 1:462507/1‑81 (MQ=255)
ccccGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGt < 2:462462/80‑1 (MQ=255)
ccGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGtttt < 2:462464/81‑1 (MQ=255)
ccGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGtttt < 2:462463/81‑1 (MQ=255)
cgcgCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGtttt > 2:462496/1‑80 (MQ=255)
gcgcTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTaa > 2:462504/1‑81 (MQ=255)
gcTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAAc > 1:462503/1‑80 (MQ=255)
ttCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACcac < 2:462465/79‑1 (MQ=255)
cGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACCACACtt < 1:462466/80‑1 (MQ=255)
cGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACCACACTTc < 1:462467/81‑1 (MQ=255)
ccTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACCACACTTCAc < 2:462468/81‑1 (MQ=255)
gacgaTCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACCACACTTCACTGcc > 1:462499/1‑81 (MQ=255)
gacgaTCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACCACACTTCACTGcc < 2:462469/81‑1 (MQ=255)
acgaTCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACCACACTTCACTGCCg < 2:462470/81‑1 (MQ=255)
ccGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACCACACTTCACTGCCGTTATCCGGCCCCATTc > 1:462509/1‑81 (MQ=255)
aCGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACCACACTTCACTGCCGTTATCCGGCCCCATTCGCTg < 1:462471/81‑1 (MQ=255)
gATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACCACACTTCACTGCCGTTATCCGGCCCCATTCGCTGc > 1:462517/1‑80 (MQ=255)
tCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACCACACTTCACTGCCGTTATCCGGCCCCATTCGCTGCAACtt < 2:462473/79‑1 (MQ=255)
tCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACCACACTTCACTGCCGTTATCCGGCCCCATTCGCTGCAACTTc < 2:462472/80‑1 (MQ=255)
tCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACCACACTTCACTGCCGTTATCCGGCCCCATTCGCTGCAACTTCa > 2:462515/1‑81 (MQ=255)
aCCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACCACACTTCACTGCCGTTATCCGGCCCCATTCGCTGCAACTTCAcc < 2:462474/81‑1 (MQ=255)
ccGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACCACACTTCACTGCCGTTATCCGGCCCCATTCGCTGCAACTTCACCa < 1:462475/81‑1 (MQ=255)
cTGCCGGGTTATAGCCTTCCAGTTTTAACCACACTTCACTGCCGTTATCCGGCCCCATTCGCTGCAACTTCACCAGAGGCg > 1:462518/1‑81 (MQ=255)
gCCGGGTTATAGCCTTCCAGTTTTAACCACACTTCACTGCCGTTATCCGGCCCCATTCGCTGCAACTTCACCAGAGGCGTa > 2:462511/1‑81 (MQ=255)
|
TTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGTTTTAACCACACTTCACTGCCGTTATCCGGCCCCATTCGCTGCAACTTCACCAGAGGCGTA > NC_000913/2539407‑2539554
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
