Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,592,993 |
C→T |
intergenic (+31/+79) |
ypfN → / ← ypfH |
UPF0370 protein YpfN/esterase YpfH |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,592,993 | 0 | C | T | 100.0%
| 132.0
/ NA
| 36 | intergenic (+31/+79) | ypfN/ypfH | UPF0370 protein YpfN/esterase YpfH |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (25/11); total (25/11) |
GGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGT > NC_000913/2592916‑2593068
|
gggACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGgtgt > 1:473835/1‑80 (MQ=255)
aCGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAAc < 2:473810/81‑1 (MQ=255)
gATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAAcgc > 2:473864/1‑81 (MQ=255)
gATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAAcgc > 1:473844/1‑81 (MQ=255)
gacgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATc < 2:473811/81‑1 (MQ=255)
acTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCgg > 1:473867/1‑80 (MQ=255)
tGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCaa > 2:473846/1‑81 (MQ=255)
gCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATg > 2:473847/1‑81 (MQ=255)
gAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGt > 1:473865/1‑80 (MQ=255)
gAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGt > 1:473862/1‑80 (MQ=255)
gATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATg > 2:473849/1‑81 (MQ=255)
tCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGcc > 1:473871/1‑81 (MQ=255)
aaCCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCt < 1:473812/80‑1 (MQ=255)
gaagaaGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGc > 2:473850/1‑81 (MQ=255)
gaagaaGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGc > 2:473848/1‑81 (MQ=255)
aagaagTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCg < 2:473813/81‑1 (MQ=255)
gaagTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGAc < 1:473814/81‑1 (MQ=255)
aagTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACg > 1:473852/1‑81 (MQ=255)
agTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGc > 1:473854/1‑81 (MQ=255)
tAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCt > 2:473857/1‑80 (MQ=255)
aGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCAc < 2:473815/81‑1 (MQ=255)
gAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGt > 1:473866/1‑81 (MQ=255)
tGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCt > 1:473869/1‑80 (MQ=255)
aaCCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCa < 2:473816/80‑1 (MQ=255)
aaCCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAg > 1:473876/1‑81 (MQ=255)
aaCCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAg > 1:473856/1‑81 (MQ=255)
aCCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAgg > 1:473855/1‑81 (MQ=255)
gTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGc > 1:473886/1‑79 (MQ=255)
aGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAgg > 2:473858/1‑81 (MQ=255)
ggCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGt < 2:473817/81‑1 (MQ=255)
gCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGt > 1:473853/1‑80 (MQ=255)
cGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAAt > 1:473863/1‑81 (MQ=255)
cGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAAt > 1:473868/1‑81 (MQ=255)
ggATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATc < 1:473818/81‑1 (MQ=255)
gATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCt > 2:473861/1‑81 (MQ=255)
aTAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTg < 1:473819/81‑1 (MQ=255)
tAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGt < 2:473820/81‑1 (MQ=255)
|
GGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGT > NC_000913/2592916‑2593068
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
