Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,827,666 |
T→A |
V102E (GTG→GAG) |
srlB → |
sorbitol‑specific PTS enzyme IIA component |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,827,666 | 0 | T | A | 100.0%
| 135.6
/ NA
| 37 | V102E (GTG→GAG) | srlB | sorbitol‑specific PTS enzyme IIA component |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (21/16); total (21/16) |
AACCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATC > NC_000913/2827590‑2827743
|
aaCCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCa < 2:520653/81‑1 (MQ=255)
ccTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAgg > 1:520710/1‑81 (MQ=255)
cTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGaggcaggc > 2:520699/1‑81 (MQ=255)
tCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGccc > 2:520705/1‑81 (MQ=255)
gcgAACTGGGTCATATCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCt < 1:520654/80‑1 (MQ=255)
cgAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGt > 2:520714/1‑81 (MQ=255)
aaCTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTcc > 1:520703/1‑81 (MQ=255)
aTGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGata > 1:520719/1‑81 (MQ=255)
aTGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGat < 1:520655/80‑1 (MQ=255)
gTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATc > 2:520708/1‑81 (MQ=255)
aCCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATcgcg < 2:520656/81‑1 (MQ=255)
cccTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCgcgc < 2:520657/81‑1 (MQ=255)
cccTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCgcgc > 2:520701/1‑81 (MQ=255)
gcgcTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCgg > 2:520720/1‑80 (MQ=255)
gcTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGAt > 1:520704/1‑81 (MQ=255)
cTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATc < 1:520658/81‑1 (MQ=255)
cTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATc < 2:520659/81‑1 (MQ=255)
gATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGtt > 2:520706/1‑81 (MQ=255)
tGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGtttt > 2:520721/1‑81 (MQ=255)
gTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGa < 1:520660/81‑1 (MQ=255)
aaaCGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGtt > 1:520717/1‑81 (MQ=255)
aaCGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGttt > 1:520707/1‑81 (MQ=255)
aaCGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGtt < 1:520661/80‑1 (MQ=255)
aGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATc > 1:520724/1‑81 (MQ=255)
aaTTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTa < 2:520662/81‑1 (MQ=255)
aTTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGtt > 1:520713/1‑79 (MQ=255)
tCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAgg < 2:520663/80‑1 (MQ=255)
tCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAg > 2:520725/1‑79 (MQ=255)
gggCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGTAGTTTGAATCTGTTAAGGAGt > 1:520715/1‑80 (MQ=255)
gggCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGCTTTGAAGTTTGAATCTGTTAAGGAGt > 1:520709/1‑80 (MQ=255)
cTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTaaaaaa < 1:520664/81‑1 (MQ=255)
tCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAAt < 2:520666/79‑1 (MQ=255)
tCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATg < 2:520665/80‑1 (MQ=255)
cATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGaa < 2:520667/80‑1 (MQ=255)
cATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAAt > 2:520718/1‑81 (MQ=255)
cATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAAt > 1:520737/1‑81 (MQ=255)
aTGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAAt > 2:520726/1‑80 (MQ=255)
aTGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATc < 1:520668/81‑1 (MQ=255)
|
AACCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATC > NC_000913/2827590‑2827743
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
