Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,832,654 |
T→A |
V60E (GTG→GAG) |
norV → |
anaerobic nitric oxide reductase flavorubredoxin |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,832,654 | 0 | T | A | 100.0%
| 98.8
/ NA
| 28 | V60E (GTG→GAG) | norV | anaerobic nitric oxide reductase flavorubredoxin |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (18/10); total (18/10) |
CAATAGCTACCTCATCCGCGAAGAAAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGTGCAGAACCTGCGTAATGAAATCGATCTGGCGGATATCGATTACATCGTGATTAACCATGCAGAAGAGGACCAC > NC_000913/2832580‑2832727
|
cAATAGCTACCTCATCCGCGAAGGAAAAAACGTGCTGATCGACACCGACGACCATAAATTCAGCCGCGAATTTGAGCAg < 2:521837/79‑1 (MQ=255)
aaTAGCTACCTCATCCGCGAAGAAAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAAc > 2:521889/1‑81 (MQ=255)
aTAGCTACCTCATCCGCGAAGAAAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAAcc > 2:521876/1‑81 (MQ=255)
tAGCTACCTCATCCGCGAAGAAAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCt < 1:521838/81‑1 (MQ=255)
aGCTACCTCATCCGCGAAGAAAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTg > 2:521888/1‑81 (MQ=255)
gCTACCTCATCCGCGAAGAAAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGc < 2:521839/81‑1 (MQ=255)
gCTACCTCATCCGCGAAGAAAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGc > 1:521873/1‑81 (MQ=255)
aCCTCATCCGCGAAGAAAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGt > 2:521867/1‑80 (MQ=255)
aCCTCATCCGCGAAGAAAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGt > 1:521870/1‑80 (MQ=255)
cTCATCCGCGAAGAAAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGTAAt > 2:521869/1‑81 (MQ=255)
cgcgAAGAAAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGTAATGAAAt < 2:521840/80‑1 (MQ=255)
cgcgAAGAAAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGTAATGAAATc > 2:521877/1‑81 (MQ=255)
gcgAAGAAAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGTAATGAAATc > 1:521885/1‑80 (MQ=255)
gaagaaAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGTAATGAAATCGAt > 1:521887/1‑81 (MQ=255)
gaagaaAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGTAATGAAATCGAt < 2:521841/81‑1 (MQ=255)
agaaAAAAACGCGCTGATCGACACCGTCGACCATAAATCCAGACGCGAATTTGAGCAGAACCTGCGTAATGAAATCGATCt > 2:521883/1‑81 (MQ=255)
gaaAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGTAATGAAATCGATCTg < 1:521842/81‑1 (MQ=255)
aaaaaaaCGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGTAATGAAATCGATCTgg > 2:521884/1‑81 (MQ=255)
aaaaaaCGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGTAATGAAATCGATCTGGc < 2:521843/81‑1 (MQ=255)
aaaaCGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGTAATGAAATCGATCTGGCg > 1:521868/1‑80 (MQ=255)
aaCGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGTAATGAAATCGATCTGGCGGat < 2:521844/81‑1 (MQ=255)
gTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGTAATGAAATCGATCTGGCGGATATc > 1:521896/1‑81 (MQ=255)
cacCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGTAATGAAATCGATCTGGCGGATATCGATTACATCGt < 2:521845/81‑1 (MQ=255)
cGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGTAATGAAATCGATCTGGCGGATATCGATTACATCGTGATTaa > 1:521875/1‑81 (MQ=255)
ccGCGAATTTGAGCAGAACCTGCGTAATGAAATCGATCTGGCGGATATCGATTACATCGTGATTAACCATGCAGAAGAGGa > 1:521907/1‑81 (MQ=255)
cgcgAATTTGAGCAGAACCTGCGTAATGAAATCGATCTGGCGGATATCGATTACATCGTGATTAACCATGCAGAAGAGGAc > 1:521874/1‑81 (MQ=255)
gcgAATTTGAGCAGAACCTGCGTAATGAAATCGATCTGGCGGATATCGATTACATCGTGATTAACCATGCAGAAGAGGAcc < 2:521846/81‑1 (MQ=255)
gAATTTGAGCAGAACCTGCGTAATGAAATCGATCTGGCGGATATCGATTACATCGTGATTAACCATGCAGAAGAGGACCAc > 2:521894/1‑81 (MQ=255)
|
CAATAGCTACCTCATCCGCGAAGAAAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGTGCAGAACCTGCGTAATGAAATCGATCTGGCGGATATCGATTACATCGTGATTAACCATGCAGAAGAGGACCAC > NC_000913/2832580‑2832727
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
