Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,016,863 |
C→A |
I268I (ATC→ATA) |
ygfK → |
putative oxidoreductase, Fe‑S subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,016,863 | 0 | C | A | 100.0%
| 104.1
/ NA
| 29 | I268I (ATC→ATA) | ygfK | putative oxidoreductase, Fe‑S subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (14/15); total (14/15) |
CATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGC > NC_000913/3016785‑3016937
|
cATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACt > 2:559753/1‑81 (MQ=255)
aTGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTc < 1:559716/81‑1 (MQ=255)
tGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATg > 1:559748/1‑81 (MQ=255)
aaaGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGt > 2:559766/1‑81 (MQ=255)
aaCACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTAc < 2:559717/81‑1 (MQ=255)
acCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACa > 1:559754/1‑79 (MQ=255)
acCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATa < 2:559718/81‑1 (MQ=255)
cTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAgg < 2:559719/81‑1 (MQ=255)
ttGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCt > 1:559760/1‑81 (MQ=255)
tgtgAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGc < 2:559720/79‑1 (MQ=255)
tgtgAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCt < 1:559721/80‑1 (MQ=255)
tgAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTa < 2:559722/80‑1 (MQ=255)
ccGACCTTACTGGGGTACTCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATATGCTTAAAAGAAGAGTCa > 2:559746/1‑81 (MQ=255)
ccGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCa > 2:559764/1‑81 (MQ=255)
cGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCAt > 1:559765/1‑81 (MQ=255)
cTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGa > 1:559781/1‑81 (MQ=255)
tACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGAt > 1:559758/1‑80 (MQ=255)
aCTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGAt < 2:559723/79‑1 (MQ=255)
aCTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATc < 1:559724/80‑1 (MQ=255)
ggTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGAcc < 1:559725/81‑1 (MQ=255)
gcgcgTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAg > 2:559783/1‑81 (MQ=255)
cgcgTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGc < 1:559726/81‑1 (MQ=255)
gcgTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCt < 2:559727/81‑1 (MQ=255)
gcgTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCt < 1:559728/81‑1 (MQ=255)
tCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGc > 2:559759/1‑80 (MQ=255)
tCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGc > 2:559775/1‑80 (MQ=255)
cGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGc > 1:559780/1‑79 (MQ=255)
tGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGc < 2:559730/81‑1 (MQ=255)
tGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGc < 1:559729/81‑1 (MQ=255)
|
CATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGC > NC_000913/3016785‑3016937
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
