Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,152,994 |
C→T |
S253S (AGC→AGT) |
metC → |
cystathionine beta‑lyase/L‑cysteine desulfhydrase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,152,994 | 0 | C | T | 100.0%
| 134.7
/ NA
| 37 | S253S (AGC→AGT) | metC | cystathionine beta‑lyase/L‑cysteine desulfhydrase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (19/18); total (19/18) |
TTGCTGGGAGCAGCTACGGGAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGCCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCTGAAAGTGGCTGAATGGCT > NC_000913/3152919‑3153065
|
ttGCTGGGAGCAGCTACGGGAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTgg > 2:590879/1‑81 (MQ=255)
gggAGCAGCTACGGGAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTgc > 1:590890/1‑81 (MQ=255)
gAGCAGCTACGGGAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTgcgc < 2:590837/81‑1 (MQ=255)
gAGCAGCTACGGGAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTgcg > 2:590875/1‑80 (MQ=255)
gcagcTACGGGAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGcac < 2:590838/81‑1 (MQ=255)
cagcTACGGGAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGcaca > 2:590880/1‑81 (MQ=255)
aCGGGAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAgg > 2:590872/1‑81 (MQ=255)
cGGGAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAg < 1:590839/79‑1 (MQ=255)
ggAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGgtgt > 2:590882/1‑81 (MQ=255)
ggAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGgtgt > 1:590888/1‑81 (MQ=255)
gAAAATGCCTATCTGATGAGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGtgtg < 1:590841/81‑1 (MQ=255)
gAAAATGCCTATCTGATCTGCCTGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGgtgag < 1:590840/81‑3 (MQ=255)
aaaTGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCg > 2:590885/1‑81 (MQ=255)
aTGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGtt < 2:590842/81‑1 (MQ=255)
ccTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTg < 1:590843/80‑1 (MQ=255)
ccTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGc < 1:590844/81‑1 (MQ=255)
gATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAAca > 2:590883/1‑81 (MQ=255)
tGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAAcatc < 1:590845/81‑1 (MQ=255)
gggCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAAcatca > 1:590887/1‑81 (MQ=255)
ggCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAAcatcat < 2:590846/81‑1 (MQ=255)
gCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAAcatcat > 2:590886/1‑80 (MQ=255)
gCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATg < 2:590847/81‑1 (MQ=255)
cAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGaa < 1:590848/81‑1 (MQ=255)
tGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCa < 1:590849/81‑1 (MQ=255)
ggTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGACAACATCATGAAAGc > 1:590850/1‑79 (MQ=255)
gTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGACAACATCATGAAAGCa < 2:590850/79‑1 (MQ=255)
cGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCt > 2:590895/1‑81 (MQ=255)
gATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCt > 2:590851/1‑80 (MQ=255)
gATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCt > 2:590891/1‑80 (MQ=255)
gATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCt > 1:590898/1‑80 (MQ=255)
gATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCt > 1:590897/1‑80 (MQ=255)
gATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCt > 1:590893/1‑80 (MQ=255)
aTGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCTGa < 2:590852/81‑1 (MQ=255)
aTACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCTGAAAGTgg < 2:590853/81‑1 (MQ=255)
tACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCTGAAAGTGGc < 1:590854/81‑1 (MQ=255)
cGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCTGAAAGTGGCTg > 2:590892/1‑80 (MQ=255)
tataACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCTGAAAGTGGCTGAAtggct < 1:590855/81‑1 (MQ=255)
|
TTGCTGGGAGCAGCTACGGGAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGCCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCTGAAAGTGGCTGAATGGCT > NC_000913/3152919‑3153065
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
