Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	315	33.5	1889570	98.7%	1865005	80.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,403,864	C→T	A127A (GCC→GCT)	yhdH →	acrylyl‑CoA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,403,864	0	C	T	100.0%	116.7 / NA	34	A127A (GCC→GCT)	yhdH	acrylyl‑CoA reductase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (20/14); total (20/14)

CGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTG  >  NC_000913/3403790‑3403939

cgAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGttt                                                                        >  2:647134/1‑80 (MQ=255)

  aGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTAc                                                                     >  2:647162/1‑81 (MQ=255)

  aGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTAc                                                                     >  1:647142/1‑81 (MQ=255)

     gAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGc                                                                  >  2:647141/1‑81 (MQ=255)

        aGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCAt                                                               <  2:647091/81‑1 (MQ=255)

         ggTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATg                                                              >  1:647137/1‑81 (MQ=255)

          gTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGc                                                             <  1:647092/81‑1 (MQ=255)

             aCTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCtgt                                                          <  1:647093/81‑1 (MQ=255)

             aCTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCtgt                                                          >  1:647165/1‑81 (MQ=255)

                    ggTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGAt                                                   >  1:647147/1‑81 (MQ=255)

                        gCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCGGTGTGTGATGgcg                                               >  1:647151/1‑81 (MQ=255)

                         ccATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGgcg                                               <  2:647094/80‑1 (MQ=255)

                            tGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTg                                            >  1:647163/1‑80 (MQ=255)

                             gCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTgg                                           >  2:647149/1‑80 (MQ=255)

                                  aaGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATg                                     <  1:647095/81‑1 (MQ=255)

                                      gCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCgg                                 >  2:647153/1‑81 (MQ=255)

                                       cTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAGGATGCCGgt                                <  1:647096/81‑1 (MQ=255)

                                         ggACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGgtgt                              >  2:647177/1‑81 (MQ=255)

                                          gACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGgtgt                              <  2:647097/80‑1 (MQ=255)

                                          gACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGtt                             >  1:647154/1‑81 (MQ=255)

                                              cgcgTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGcc                         <  1:647098/81‑1 (MQ=255)

                                                cgTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCg                       >  1:647159/1‑81 (MQ=255)

                                                cgTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCg                       <  1:647099/81‑1 (MQ=255)

                                                 gTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCg                       >  2:647160/1‑80 (MQ=255)

                                                   aaaGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAg                    >  1:647152/1‑81 (MQ=255)

                                                    aaGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAg                    <  1:647100/80‑1 (MQ=255)

                                                    aaGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAg                    <  1:647101/80‑1 (MQ=255)

                                                      gCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGAc                 <  2:647103/81‑1 (MQ=255)

                                                      gCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGAc                 <  2:647102/81‑1 (MQ=255)

                                                                  ggTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGt      <  1:647104/80‑1 (MQ=255)

                                                                  ggTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTc     >  1:647176/1‑81 (MQ=255)

                                                                   gTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCg    >  1:647173/1‑81 (MQ=255)

                                                                   gTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCg    >  2:647157/1‑81 (MQ=255)

                                                                     aCTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGt   >  2:647158/1‑80 (MQ=255)

                                                                      ctgctgGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTg  <  1:647105/80‑1 (MQ=255)

CGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTG  >  NC_000913/3403790‑3403939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A