Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,499,007 |
C→T |
L394L (CTG→TTG) |
cysG → |
siroheme synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,499,007 | 0 | C | T | 100.0%
| 113.0
/ NA
| 32 | L394L (CTG→TTG) | cysG | siroheme synthase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (13/19); total (13/19) |
GGAAAACCTGGCGGCAGAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCAGCGCGTGA > NC_000913/3498931‑3499082
|
ggAAAACCTGGCGGCAGAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTg < 2:668527/79‑1 (MQ=255)
ggAAAACCTGGCGGCAGAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGAt < 1:668526/81‑1 (MQ=255)
aaCCTGGCGGCAGAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGaa > 2:668562/1‑81 (MQ=255)
tGGCGGCAGAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACg > 1:668573/1‑81 (MQ=255)
ggcggcAGAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACg > 2:668571/1‑80 (MQ=255)
gcggcAGAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACgg < 1:668528/80‑1 (MQ=255)
gcggcAGAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGa < 2:668529/81‑1 (MQ=255)
cggcAGAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGaa > 1:668567/1‑81 (MQ=255)
ggcAGAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAAt < 1:668530/81‑1 (MQ=255)
gAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCa < 1:668531/81‑1 (MQ=255)
aaCAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGc > 2:668563/1‑80 (MQ=255)
aGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGaaa < 1:668532/81‑1 (MQ=255)
gACGCTGGTGTTCTATATGGGGTTGAATCAGACCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAAt < 2:668533/81‑1 (MQ=255)
gCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGcc > 1:668566/1‑81 (MQ=255)
ggTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGt > 2:668568/1‑81 (MQ=255)
ttCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGc > 1:668570/1‑80 (MQ=255)
ttCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCa < 1:668534/81‑1 (MQ=255)
tatGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAAtt < 1:668536/79‑1 (MQ=255)
tatGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAAt < 1:668535/78‑1 (MQ=255)
tGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTc < 2:668537/80‑1 (MQ=255)
gggTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGa > 2:668581/1‑80 (MQ=255)
ttGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAAc < 2:668538/81‑1 (MQ=255)
gAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACgg > 2:668574/1‑81 (MQ=255)
aGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACg < 1:668539/80‑1 (MQ=255)
gACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCAc < 2:668540/81‑1 (MQ=255)
gACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCAc > 2:668585/1‑81 (MQ=255)
tATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACg > 1:668586/1‑79 (MQ=255)
ttCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCa < 2:668541/79‑1 (MQ=255)
ttCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCAg < 2:668542/80‑1 (MQ=255)
tCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCAgcg > 2:668584/1‑81 (MQ=255)
aggaaaaGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCAgcgc < 2:668543/77‑1 (MQ=255)
aaaaGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCAGCGCGTGa < 1:668544/81‑1 (MQ=255)
|
GGAAAACCTGGCGGCAGAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCAGCGCGTGA > NC_000913/3498931‑3499082
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
