Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I2 R1	12	27.9	1984062	98.2%	1948348	80.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,184,972	T→A	Q208L (CAG→CTG)	potA ←	spermidine preferential ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,184,972	0	T	A	100.0%	73.3 / NA	20	Q208L (CAG→CTG)	potA	spermidine preferential ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (11/9); total (11/9)

TCACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACG  >  NC_000913/1184894‑1185048

tCACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAgg                                                                            >  1:197004/1‑81 (MQ=255)

  aCGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTc                                                                          >  1:197017/1‑81 (MQ=255)

         gTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGt                                                                    >  1:197010/1‑80 (MQ=255)

                   gCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGAc                                                           <  2:196976/79‑1 (MQ=255)

                   gCTCAATGCGACCATCGCGAATCACCACGATCCTGTCTGACATGGTGAGTGCTTGTTCCAGGTCGTAAGTCACAAAGACg                                                          <  2:196975/80‑1 (MQ=255)

                         tGCGACCATCGCGCATCACCACAATTCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTaa                                                   <  2:196977/81‑1 (MQ=255)

                            gACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGc                                                >  2:197021/1‑81 (MQ=255)

                              ccATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGcc                                               >  1:197011/1‑80 (MQ=255)

                                       aTCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGc                                     >  1:197016/1‑81 (MQ=255)

                                           ccacAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGt                                  >  1:197033/1‑80 (MQ=255)

                                           ccacAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTa                                 <  1:196979/81‑1 (MQ=255)

                                           ccacAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTa                                 <  2:196978/81‑1 (MQ=255)

                                                   cTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTc                         <  2:196980/81‑1 (MQ=255)

                                                          aCATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTc                    <  2:196982/79‑1 (MQ=255)

                                                          aCATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCg                   <  1:196981/80‑1 (MQ=255)

                                                            aTGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTc                <  2:196983/81‑1 (MQ=255)

                                                              ggTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTg              >  1:197019/1‑81 (MQ=255)

                                                                      cttcttCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCt      >  2:197025/1‑81 (MQ=255)

                                                                       ttcttcCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCt      >  1:197031/1‑80 (MQ=255)

                                                                           tcCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACg  >  2:197029/1‑80 (MQ=255)

TCACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACG  >  NC_000913/1184894‑1185048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A