Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
13 |
33.8 |
2116282 |
98.9% |
2093002 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,184,972 |
T→A |
Q208L (CAG→CTG) |
potA ← |
spermidine preferential ABC transporter ATP binding subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,184,972 | 0 | T | A | 100.0%
| 140.0
/ NA
| 36 | Q208L (CAG→CTG) | potA | spermidine preferential ABC transporter ATP binding subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (19/17); total (19/17) |
CACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTAC > NC_000913/1184895‑1185047
|
cACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGt > 2:227174/1‑81 (MQ=255)
aCGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGt < 2:227136/80‑1 (MQ=255)
gcggcgTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGt < 1:227137/81‑1 (MQ=255)
ggcgTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGt > 2:227172/1‑79 (MQ=255)
cgTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGt < 2:227138/81‑1 (MQ=255)
gTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTc < 1:227139/81‑1 (MQ=255)
ccGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTcaca < 2:227140/81‑1 (MQ=255)
cGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTcaca > 2:227176/1‑80 (MQ=255)
cGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACaa < 1:227141/81‑1 (MQ=255)
gTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACaaa < 2:227142/81‑1 (MQ=255)
ttGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGAc > 1:227211/1‑81 (MQ=255)
cGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATg > 1:227185/1‑81 (MQ=255)
gACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTTAGTCACAAAGACGAATGTAATGc > 1:227188/1‑81 (MQ=255)
gACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGc < 1:227143/81‑1 (MQ=255)
ccATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCa < 2:227144/81‑1 (MQ=255)
aTCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAg > 1:227186/1‑81 (MQ=255)
cgcgCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCt > 1:227184/1‑81 (MQ=255)
cgcATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTa > 2:227179/1‑81 (MQ=255)
cATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACg > 2:227191/1‑81 (MQ=255)
aTCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGc > 1:227178/1‑81 (MQ=255)
accacAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTg > 2:227190/1‑80 (MQ=255)
ccacAATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGt > 2:227197/1‑80 (MQ=255)
acaATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAAc < 1:227145/81‑1 (MQ=255)
acaATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAAc < 1:227146/81‑1 (MQ=255)
acaATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAAc > 2:227180/1‑81 (MQ=255)
acaATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAAc < 1:227147/81‑1 (MQ=255)
caATCCTGTCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACg < 2:227148/81‑1 (MQ=255)
tCTGACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCa < 2:227149/79‑1 (MQ=255)
gACATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCg > 1:227192/1‑81 (MQ=255)
aCATGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCg < 1:227150/80‑1 (MQ=255)
aTGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTc > 2:227170/1‑81 (MQ=255)
aTGGTGAGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTc > 2:227193/1‑81 (MQ=255)
aGTGCTTCTTCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCAtt < 1:227151/81‑1 (MQ=255)
cttcttCCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGc < 2:227152/80‑1 (MQ=255)
cttcCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTAc > 1:227201/1‑81 (MQ=255)
cttcCAGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTAc > 1:227187/1‑81 (MQ=255)
|
CACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTAC > NC_000913/1184895‑1185047
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
