Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I2 R1 12 27.9 1984062 98.2% 1948348 80.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,770,180 T→C N369N (AAT→AAC)  ydiK → putative transporter YdiK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,770,1800TC100.0% 64.8 / NA 18N369N (AAT→AAC) ydiKputative transporter YdiK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/5);  total (13/5)

AGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCC  >  NC_000913/1770111‑1770253
                                                                     |                                                                         
agaagTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTtct                                                                >  1:286478/1‑81 (MQ=255)
    gTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCtcat                                                             >  2:286488/1‑80 (MQ=255)
     tCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCtcat                                                             >  1:286496/1‑79 (MQ=255)
        cgccgccgACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAggc                                                        >  1:286486/1‑81 (MQ=255)
         gccgccgACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAggcg                                                       >  1:286487/1‑81 (MQ=255)
                aCGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTgc                                                >  1:286477/1‑81 (MQ=255)
                aCGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTgc                                                >  2:286475/1‑81 (MQ=255)
                  ggACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTgccg                                              >  1:286481/1‑81 (MQ=255)
                      cAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTg                                          <  2:286452/81‑1 (MQ=255)
                             aagaaATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGtt                                    >  2:286484/1‑80 (MQ=255)
                                   ttCTCGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAaccacc                             <  1:286453/81‑1 (MQ=255)
                                     ctcGAAGAACTTGGCGAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGc                           >  2:286483/1‑81 (MQ=255)
                                                   cGAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATttgc             <  1:286455/81‑1 (MQ=255)
                                                   cGAAATCGAGAAACCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATttgc             <  1:286454/81‑1 (MQ=255)
                                                          gagaAACCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGctct      >  2:286480/1‑81 (MQ=255)
                                                             aaaCCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTcc   >  1:286494/1‑81 (MQ=255)
                                                             aaaCCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTcc   >  2:286485/1‑81 (MQ=255)
                                                              aaCCGAACAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTccc  <  2:286456/81‑1 (MQ=255)
                                                                     |                                                                         
AGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCC  >  NC_000913/1770111‑1770253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A