Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I2 R1
|
12 |
27.9 |
1984062 |
98.2% |
1948348 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,471,736 |
A→T |
F593L (TTT→TTA) |
fusA ← |
elongation factor G |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,471,736 | 0 | A | T | 100.0%
| 143.5
/ NA
| 38 | F593L (TTT→TTA) | fusA | elongation factor G |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (21/17); total (21/17) |
TGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTCTACTACCGGGTAGCC > NC_000913/3471666‑3471813
|
tGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGtt > 2:613383/1‑81 (MQ=255)
tGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGtt > 1:613388/1‑81 (MQ=255)
gCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAgagg < 2:613332/81‑1 (MQ=255)
cAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGt > 2:613404/1‑81 (MQ=255)
aaGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCGGAGGAGTc < 2:613333/81‑1 (MQ=255)
aGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACg > 1:613392/1‑81 (MQ=255)
gAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGt > 2:613389/1‑81 (MQ=255)
gAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGt > 1:613407/1‑81 (MQ=255)
gTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGt < 2:613335/81‑1 (MQ=255)
gTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTAATGGt < 1:613334/81‑1 (MQ=255)
tttCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTa < 2:613336/81‑1 (MQ=255)
ttCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGt > 2:613378/1‑79 (MQ=255)
ttCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGt > 1:613399/1‑79 (MQ=255)
cGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAg < 1:613337/80‑1 (MQ=255)
gCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGaa < 2:613338/81‑1 (MQ=255)
gCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGa < 2:613339/80‑1 (MQ=255)
tCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAAcc < 2:613341/79‑1 (MQ=255)
tCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCg < 2:613340/80‑1 (MQ=255)
tCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCg > 1:613386/1‑80 (MQ=255)
cTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCg > 1:613428/1‑79 (MQ=255)
aaaGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAg < 1:613342/79‑1 (MQ=255)
aaaGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTg > 1:613395/1‑81 (MQ=255)
aaGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTg > 2:613411/1‑80 (MQ=255)
gCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAg < 2:613343/81‑1 (MQ=255)
tcttTAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACg > 1:613391/1‑80 (MQ=255)
tAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAAt < 1:613344/79‑1 (MQ=255)
tAAAGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATAc > 2:613410/1‑81 (MQ=255)
aaaGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATAcc > 1:613402/1‑81 (MQ=255)
aGGCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCa > 1:613400/1‑81 (MQ=255)
ggCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCAt < 2:613345/81‑1 (MQ=255)
gCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATg > 1:613397/1‑81 (MQ=255)
gCGATAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATg < 2:613346/81‑1 (MQ=255)
aTAGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTct < 2:613347/81‑1 (MQ=255)
aGAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTctac > 1:613393/1‑81 (MQ=255)
gAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTctact < 1:613348/81‑1 (MQ=255)
gAAGCAGCCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTctac > 2:613394/1‑80 (MQ=255)
agcCAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTCTACTACCgg > 2:613398/1‑81 (MQ=255)
cAGTTTTAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCTATGTCTACTACCggg > 1:613425/1‑79 (MQ=255)
ttttAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTCTACTACCGGGTAGcc < 1:613349/81‑1 (MQ=255)
ttttAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTCTACTACCGGGTAGcc < 1:613350/81‑1 (MQ=255)
|
TGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCGAAGTGCAGACGAATACCCATGTCTACTACCGGGTAGCC > NC_000913/3471666‑3471813
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
