Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F12 I0 R1
|
23 |
94.9 |
5204302 |
90.2% |
4694280 |
99.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
3,535,733 |
A→C |
100% |
F46V (TTT→GTT) |
envZ ← |
sensory histidine kinase EnvZ |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,535,733 | 0 | A | C | 100.0%
| 271.3
/ NA
| 81 | F46V (TTT→GTT) | envZ | sensory histidine kinase EnvZ |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (42/39); total (42/39) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAA > NC_000913/3535637‑3535823
|
gACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACct > 1:2532175/1‑99 (MQ=255)
gACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACct > 2:1891295/1‑99 (MQ=255)
aCGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACctg > 2:769013/1‑99 (MQ=255)
aCGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACctg > 1:263165/1‑99 (MQ=255)
aCGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACctg < 2:2036647/99‑1 (MQ=255)
ggAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACctgct > 2:1369187/1‑99 (MQ=255)
ggAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTACTAACctgct < 1:149358/99‑1 (MQ=255)
aGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAg < 2:1790811/99‑1 (MQ=255)
gCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAgg > 1:2043174/1‑99 (MQ=255)
cGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGc < 1:1565910/99‑1 (MQ=255)
gggAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCt < 1:2031097/99‑1 (MQ=255)
ggAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTc > 1:1850435/1‑99 (MQ=255)
gAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCg > 1:1067855/1‑99 (MQ=255)
gCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCa > 2:590714/1‑99 (MQ=255)
caaccaacTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCaaaa < 2:2054160/99‑1 (MQ=255)
caaccaacTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCaaaa > 2:13629/1‑99 (MQ=255)
caaccaacTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCaaaa > 2:1163275/1‑99 (MQ=255)
caaccaacTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCaaaa > 1:1250658/1‑99 (MQ=255)
caaccaacTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCaaaa < 1:1990426/99‑1 (MQ=255)
accaacTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATc > 2:2055013/1‑99 (MQ=255)
accaacTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATc > 2:955219/1‑99 (MQ=255)
aacTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATcgcg < 2:967752/99‑1 (MQ=255)
cTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGaa > 2:1119087/1‑99 (MQ=255)
cTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGaa < 1:799107/99‑1 (MQ=255)
cGTCCTCCAGTTGCAGTTTGTCGTTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGca < 2:855491/99‑1 (MQ=255)
cGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGca < 1:816110/99‑1 (MQ=255)
cGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGca > 1:772215/1‑99 (MQ=255)
gTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGcac < 2:436323/99‑1 (MQ=255)
tcctccAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGcacc > 2:1465436/1‑99 (MQ=255)
ctccAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGcaccac < 1:1025438/99‑1 (MQ=255)
tccAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCaccacc > 2:2436711/1‑99 (MQ=255)
ccAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCAccacca < 2:1161624/99‑1 (MQ=255)
ttGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATa < 1:1803764/99‑1 (MQ=255)
ttGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATa > 2:1698344/1‑99 (MQ=255)
gCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAg < 1:1182757/99‑1 (MQ=255)
cAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAgt < 2:18957/99‑1 (MQ=255)
tttGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAgtcgt > 1:144532/1‑99 (MQ=255)
tttGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAgtcgt > 2:1912227/1‑99 (MQ=255)
ttGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAgtcgtc < 1:925581/99‑1 (MQ=255)
gTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCAc > 1:652057/1‑99 (MQ=255)
ggTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAg < 1:1385386/99‑1 (MQ=255)
gTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAgg < 2:2446100/99‑1 (MQ=255)
gTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAgg > 1:2226381/1‑99 (MQ=255)
tcatcaACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGTAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGc < 2:860777/99‑1 (MQ=255)
tcatcaACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGc < 2:1113811/99‑1 (MQ=255)
tcatcaACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGc < 2:1506849/99‑1 (MQ=255)
tcatcaACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGc < 2:1295509/99‑1 (MQ=255)
tcaACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTgg < 2:676887/99‑1 (MQ=255)
tcaACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTgg > 1:1895557/1‑99 (MQ=255)
tcaACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTgg < 2:2573714/99‑1 (MQ=255)
aCATACGCACTTCGTACGCGAGGACTTTTTTAACCTCCTGGAGCCTCGCCAAAATCGCGAAGTTCGGCACCACCAGAAAAGTCGCCACCAGGCTGGCGa > 1:2025291/1‑99 (MQ=255)
aCATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGa > 1:1614675/1‑99 (MQ=255)
cATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGaa < 2:1448130/99‑1 (MQ=255)
aTACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAAc < 1:1734504/99‑1 (MQ=255)
taggCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACa > 1:1606723/4‑99 (MQ=255)
tACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACa > 1:1606722/1‑99 (MQ=255)
tACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACa > 1:742768/1‑99 (MQ=255)
tACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACa < 1:827181/99‑1 (MQ=255)
tACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACa > 2:36722/1‑99 (MQ=255)
cGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGc < 1:962421/99‑1 (MQ=255)
aCTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAg < 1:629216/99‑1 (MQ=255)
cTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAgg > 1:2480170/1‑99 (MQ=255)
ttCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGt > 1:1294911/1‑99 (MQ=255)
cGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGa < 2:2498635/99‑1 (MQ=255)
tACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACg > 1:1444801/1‑99 (MQ=255)
tACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACg > 1:1306751/1‑99 (MQ=255)
tACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACg > 1:1706212/1‑99 (MQ=255)
tACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACg > 2:1849401/1‑99 (MQ=255)
tACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACg > 1:1311373/1‑99 (MQ=255)
tACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACg > 2:2541750/1‑99 (MQ=255)
aCGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGa < 2:1391450/99‑1 (MQ=255)
aCGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGa < 1:1347398/99‑1 (MQ=255)
aCGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGa < 1:948133/99‑1 (MQ=255)
aCGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGa < 2:1750289/99‑1 (MQ=255)
gAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAg > 2:1509947/1‑99 (MQ=255)
gAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAg > 1:2355773/1‑99 (MQ=255)
aGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGc > 1:942210/1‑99 (MQ=255)
ggACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCa < 1:2228945/99‑1 (MQ=255)
gACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCaa > 2:2017093/1‑99 (MQ=255)
gACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCaa < 2:1552501/99‑1 (MQ=255)
tttATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATaa < 1:190438/99‑1 (MQ=255)
|
GACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAA > NC_000913/3535637‑3535823
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
