Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F12 I0 R1	23	116.7	6197560	91.2%	5652174	99.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,535,618	A→T	100%	L84Q (CTG→CAG)	envZ ←	sensory histidine kinase EnvZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,535,618	0	A	T	100.0%	373.3 / NA	105	L84Q (CTG→CAG)	envZ	sensory histidine kinase EnvZ
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (61/44); total (61/44)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 105 total aligned reads displayed.

GTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCG  >  NC_000913/3535523‑3535713

gTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCt                                                                                              >  1:597221/1‑99 (MQ=255)

 ttGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTc                                                                                             <  1:434970/99‑1 (MQ=255)

 ttGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTc                                                                                             >  2:2341855/1‑99 (MQ=255)

 ttGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTc                                                                                             >  2:119981/1‑99 (MQ=255)

  tgctgcGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCa                                                                                            <  1:894416/99‑1 (MQ=255)

   gctgcGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCAc                                                                                           >  2:1609701/1‑99 (MQ=255)

     tgcGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACgg                                                                                         >  1:2685620/1‑99 (MQ=255)

       cgcCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTa                                                                                       <  1:2583103/99‑1 (MQ=255)

       cgcCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTa                                                                                       <  1:2092005/99‑1 (MQ=255)

           aTCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGAtc                                                                                   <  1:78297/99‑1 (MQ=255)

           aTCTGATGGCTTAAGAATTAATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGCGTAGAGAGAGAGCCCCTGCTCACGGTAGAtc                                                                                   >  2:2636062/1‑99 (MQ=255)

             cTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGAtctc                                                                                 <  2:2322230/99‑1 (MQ=255)

              tGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTcc                                                                                >  2:2888181/1‑99 (MQ=255)

               gATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTccc                                                                               <  1:393303/99‑1 (MQ=255)

               gATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTccc                                                                               >  1:1989654/1‑99 (MQ=255)

               gATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTccc                                                                               <  2:2193705/99‑1 (MQ=255)

                 tGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGa                                                                             <  1:2938595/99‑1 (MQ=255)

                 tGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGa                                                                             <  1:2137087/99‑1 (MQ=255)

                 tGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGa                                                                             <  2:1247652/99‑1 (MQ=255)

                   gCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACg                                                                           >  1:2684864/1‑99 (MQ=255)

                     ttAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGa                                                                         >  2:444189/1‑99 (MQ=255)

                     ttAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGa                                                                         >  1:55519/1‑99 (MQ=255)

                       aaGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGaaa                                                                       <  2:1817951/99‑1 (MQ=255)

                        aGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAg                                                                      <  2:2817459/99‑1 (MQ=255)

                         gAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGc                                                                     >  2:574496/1‑99 (MQ=255)

                         gAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGc                                                                     >  2:1749731/1‑99 (MQ=255)

                          aaTTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCg                                                                    <  1:1157627/99‑1 (MQ=255)

                           aTTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCgg                                                                   >  1:1715896/1‑99 (MQ=255)

                           aTTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCgg                                                                   >  2:2834973/1‑99 (MQ=255)

                            ttCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCggg                                                                  >  2:785412/1‑99 (MQ=255)

                            ttCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCggg                                                                  >  1:1844439/1‑99 (MQ=255)

                            ttCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTACCGACGGAAAGCggg                                                                  >  1:1047779/1‑99 (MQ=255)

                            ttCATAGTCTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCACCTTGGCGTATAGAGAGATTTTCTGTTCACGGTAGATCTCCCGACCGAAAACggg                                                                  >  2:92622/1‑99 (MQ=255)

                              cATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAg                                                                >  2:211829/1‑99 (MQ=255)

                               aTAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAgg                                                               >  2:2703151/1‑99 (MQ=255)

                                tAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGc                                                              >  2:1314391/1‑99 (MQ=255)

                                tAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGc                                                              >  1:2061388/1‑99 (MQ=255)

                                    gtTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCAcaa                                                          <  1:754449/99‑1 (MQ=255)

                                           ccAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTg                                                   >  2:2037622/1‑99 (MQ=255)

                                            cAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGc                                                  <  1:880722/99‑1 (MQ=255)

                                            cAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGc                                                  <  1:1068738/99‑1 (MQ=255)

                                             aaCGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCg                                                 >  2:2879656/1‑99 (MQ=255)

                                              aCGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGt                                                >  1:221476/1‑99 (MQ=255)

                                              aCGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGt                                                >  1:612596/1‑99 (MQ=255)

                                              aCGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGt                                                >  1:496663/1‑99 (MQ=255)

                                               cGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTg                                               <  2:2422400/99‑1 (MQ=255)

                                                gCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGc                                              <  1:1825926/99‑1 (MQ=255)

                                                  aGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCg                                            <  2:2427937/99‑1 (MQ=255)

                                                   gACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGt                                           <  1:2637181/99‑1 (MQ=255)

                                                   gACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGt                                           >  1:2586069/1‑99 (MQ=255)

                                                   gACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGt                                           >  1:1133069/1‑99 (MQ=255)

                                                   gACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGt                                           >  2:918819/1‑99 (MQ=255)

                                                    aCCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtc                                          <  1:89974/99‑1 (MQ=255)

                                                      cTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtcct                                        <  1:947325/99‑1 (MQ=255)

                                                       tGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtcctc                                       >  1:1986345/1‑99 (MQ=255)

                                                       tGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtcctc                                       <  2:875199/99‑1 (MQ=255)

                                                           tctTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGt                                   >  2:2475233/1‑99 (MQ=255)

                                                             ttCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTg                                 >  1:1069694/1‑99 (MQ=255)

                                                              tCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGc                                <  1:2968404/99‑1 (MQ=255)

                                                              tCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGc                                <  2:2826466/99‑1 (MQ=255)

                                                                ggCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAg                              >  2:2548576/1‑99 (MQ=255)

                                                                  cAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGtt                            >  2:1292884/1‑99 (MQ=255)

                                                                  cAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGAGCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCAAGTTGCAGtt                            <  2:2471086/99‑1 (MQ=255)

                                                                   aGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGttt                           >  1:603160/1‑99 (MQ=255)

                                                                   aGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGttt                           <  2:2743215/99‑1 (MQ=255)

                                                                    gCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTg                          >  2:1072112/1‑99 (MQ=255)

                                                                     ccTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGt                         >  2:581858/1‑99 (MQ=255)

                                                                       tCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCg                       >  2:861813/1‑99 (MQ=255)

                                                                         gTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGt                     >  1:2674212/1‑99 (MQ=255)

                                                                          ttGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtc                    <  2:353983/99‑1 (MQ=255)

                                                                           tGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTgtcagtca                   <  1:733415/99‑1 (MQ=255)

                                                                           tGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtca                   <  2:771650/99‑1 (MQ=255)

                                                                           tGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtca                   <  2:1796330/99‑1 (MQ=255)

                                                                            ggAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtcat                  >  2:1408695/1‑99 (MQ=255)

                                                                             gAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtcatc                 <  2:525902/99‑1 (MQ=255)

                                                                             gAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtcatc                 >  2:1641573/1‑99 (MQ=255)

                                                                               gTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCaa               >  2:1080777/1‑99 (MQ=255)

                                                                                 agagagagATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACa             <  1:1356382/99‑1 (MQ=255)

                                                                                 agagagagATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACa             <  1:716011/99‑1 (MQ=255)

                                                                                   agagagaTCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATa           <  1:3097908/99‑1 (MQ=255)

                                                                                   agagagaTCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATa           >  2:2174391/1‑99 (MQ=255)

                                                                                   agagagaTCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATa           <  1:2437844/99‑1 (MQ=255)

                                                                                   agagagaTCCCCTGCTCACAGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATa           <  1:683534/99‑1 (MQ=255)

                                                                                    gagagaTCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATAc          >  1:212391/1‑99 (MQ=255)

                                                                                    gagagaTCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATAc          >  1:1818632/1‑99 (MQ=255)

                                                                                    gagagaTCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATAc          <  1:1350713/99‑1 (MQ=255)

                                                                                      gagaTCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGc        >  2:2952131/1‑99 (MQ=255)

                                                                                      gagaTCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGc        >  2:56714/1‑99 (MQ=255)

                                                                                       agaTCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCa       >  1:207454/1‑99 (MQ=255)

                                                                                        gaTCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCAc      >  2:2356738/1‑99 (MQ=255)

                                                                                        gaTCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCAc      >  2:2313864/1‑99 (MQ=255)

                                                                                        gaTCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCAc      >  2:1254633/1‑99 (MQ=255)

                                                                                         aTCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACt     <  2:1898508/99‑1 (MQ=255)

                                                                                         aTCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACt     <  1:569103/99‑1 (MQ=255)

                                                                                         aTCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACt     <  1:2751159/99‑1 (MQ=255)

                                                                                          tCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACtt    >  1:861213/1‑99 (MQ=255)

                                                                                          tCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACtt    >  1:1710165/1‑99 (MQ=255)

                                                                                          tCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACtt    >  2:2384448/1‑99 (MQ=255)

                                                                                            cccTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCGTCAACATACGCACTTCg  >  2:1056474/1‑99 (MQ=255)

                                                                                            cccTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCg  >  2:2205603/1‑99 (MQ=255)

GTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCG  >  NC_000913/3535523‑3535713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A