Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F8 I0 R1	23	110.3	5688336	96.8%	5506309	99.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,535,618	A→T	100%	L84Q (CTG→CAG)	envZ ←	sensory histidine kinase EnvZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,535,618	0	A	T	100.0%	414.7 / NA	105	L84Q (CTG→CAG)	envZ	sensory histidine kinase EnvZ
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (53/52); total (53/52)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 105 total aligned reads displayed.

GTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCG  >  NC_000913/3535523‑3535713

gTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCt                                                                                              >  2:549481/1‑99 (MQ=255)

gTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCt                                                                                              <  2:1062894/99‑1 (MQ=255)

gTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCt                                                                                              >  2:1610035/1‑99 (MQ=255)

 ttGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTc                                                                                             >  2:2119698/1‑99 (MQ=255)

     tgcGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACgg                                                                                         <  2:632243/99‑1 (MQ=255)

         ccATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGa                                                                                     <  1:2439269/99‑1 (MQ=255)

         ccATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGa                                                                                     >  2:1373628/1‑99 (MQ=255)

         ccATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGa                                                                                     <  1:143964/99‑1 (MQ=255)

         ccATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGa                                                                                     >  1:321129/1‑99 (MQ=255)

         ccATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGa                                                                                     <  2:2819230/99‑1 (MQ=255)

          cATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGAt                                                                                    <  2:2229262/99‑1 (MQ=255)

          cATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGAt                                                                                    <  2:785720/99‑1 (MQ=255)

            tCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGAtct                                                                                  >  2:1759948/1‑99 (MQ=255)

            tCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGAtct                                                                                  >  1:2673206/1‑99 (MQ=255)

            tCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGAtct                                                                                  >  1:1600468/1‑99 (MQ=255)

             cTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGAtctc                                                                                 <  1:2751971/99‑1 (MQ=255)

              tGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTcc                                                                                <  1:239001/99‑1 (MQ=255)

              tGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTcc                                                                                >  2:2054003/1‑99 (MQ=255)

              tGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTcc                                                                                <  2:1370850/99‑1 (MQ=255)

                 tGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGa                                                                             <  1:1295236/99‑1 (MQ=255)

                    cTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACgg                                                                          >  1:952426/1‑99 (MQ=255)

                        aGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAg                                                                      <  1:1817163/99‑1 (MQ=255)

                        aGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAg                                                                      <  2:2586091/99‑1 (MQ=255)

                         gAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGc                                                                     >  2:743699/1‑99 (MQ=255)

                         gAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGc                                                                     <  1:2717923/99‑1 (MQ=255)

                         gAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGc                                                                     >  2:2105415/1‑99 (MQ=255)

                           aTTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCgg                                                                   >  1:1936247/1‑99 (MQ=255)

                           aTTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCgg                                                                   >  1:1140678/1‑99 (MQ=255)

                            ttCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCggg                                                                  >  1:2018519/1‑99 (MQ=255)

                            ttCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCggg                                                                  >  2:2238850/1‑99 (MQ=255)

                                tAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGc                                                              >  2:2484492/1‑99 (MQ=255)

                                  gtgtTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGcac                                                            >  2:2043281/1‑99 (MQ=255)

                                  gtgtTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGcac                                                            <  1:1702743/99‑1 (MQ=255)

                                  gtgtTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGcac                                                            >  1:177567/1‑99 (MQ=255)

                                  gtgtTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGcac                                                            >  1:2002283/1‑99 (MQ=255)

                                   tgtTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGcaca                                                           <  2:2708233/99‑1 (MQ=255)

                                    gtTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCAcaa                                                          <  1:810532/99‑1 (MQ=255)

                                         gcCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCAcaaccaac                                                     <  1:2242619/99‑1 (MQ=255)

                                         gcCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCAcaaccaac                                                     <  1:533013/99‑1 (MQ=255)

                                           ccAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTg                                                   <  1:1293766/99‑1 (MQ=255)

                                            cAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGc                                                  >  2:266989/1‑99 (MQ=255)

                                              aCGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGt                                                <  1:1247612/99‑1 (MQ=255)

                                               cGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTg                                               >  1:2197142/1‑99 (MQ=255)

                                               cGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTg                                               >  2:2330357/1‑99 (MQ=255)

                                                gCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGc                                              <  2:435177/99‑1 (MQ=255)

                                                gCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGc                                              <  1:1343437/99‑1 (MQ=255)

                                                gCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGc                                              <  1:2149097/99‑1 (MQ=255)

                                                 cAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGcc                                             >  1:775846/1‑99 (MQ=255)

                                                  aGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCg                                            >  2:1573687/1‑99 (MQ=255)

                                                   gACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGt                                           <  2:338429/99‑1 (MQ=255)

                                                   gACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGt                                           >  1:883973/1‑99 (MQ=255)

                                                    aCCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCTACTGCGTGCCGtc                                          <  1:2022024/99‑1 (MQ=255)

                                                     ccTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtcc                                         >  1:1867547/1‑99 (MQ=255)

                                                      cTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtcct                                        >  1:1254592/1‑99 (MQ=255)

                                                        gCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtcctcc                                      <  2:813037/99‑1 (MQ=255)

                                                        gCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtcctcc                                      >  1:1164690/1‑99 (MQ=255)

                                                        gCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtcctcc                                      <  1:233230/99‑1 (MQ=255)

                                                        gCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtcctcc                                      >  1:2727549/1‑99 (MQ=255)

                                                         ccTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCa                                     <  2:307970/99‑1 (MQ=255)

                                                           tctTCGGCAGCCTCGTTGGAGTAGAGAGAGATTCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGt                                   <  2:2628586/99‑1 (MQ=255)

                                                           tctTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGt                                   <  2:2106743/99‑1 (MQ=255)

                                                           tctTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGt                                   <  1:912591/99‑1 (MQ=255)

                                                           tctTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGt                                   >  2:2475623/1‑99 (MQ=255)

                                                             ttCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTg                                 >  1:1684732/1‑99 (MQ=255)

                                                               cGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCa                               <  1:2712430/99‑1 (MQ=255)

                                                                ggCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAg                              <  1:1005325/99‑1 (MQ=255)

                                                                 gCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGt                             >  1:2182416/1‑99 (MQ=255)

                                                                 gCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGt                             >  2:1029670/1‑99 (MQ=255)

                                                                 gCAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGt                             >  1:2617056/1‑99 (MQ=255)

                                                                  cAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGtt                            <  2:939089/99‑1 (MQ=255)

                                                                  cAGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGtt                            <  2:1970417/99‑1 (MQ=255)

                                                                   aGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGttt                           <  1:1231280/99‑1 (MQ=255)

                                                                   aGCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGttt                           <  2:1197403/99‑1 (MQ=255)

                                                                    gCCTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTg                          >  2:1292951/1‑99 (MQ=255)

                                                                     ccTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGt                         <  1:2062165/99‑1 (MQ=255)

                                                                      cTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTc                        >  1:1195402/1‑99 (MQ=255)

                                                                      cTCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTc                        <  1:2242308/99‑1 (MQ=255)

                                                                       tCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCg                       >  1:692987/1‑99 (MQ=255)

                                                                       tCGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCg                       <  2:664986/99‑1 (MQ=255)

                                                                        cGTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCgg                      <  1:422166/99‑1 (MQ=255)

                                                                         gTTGGAGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGt                     <  2:1685972/99‑1 (MQ=255)

                                                                              aGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtcatca                <  1:2718861/99‑1 (MQ=255)

                                                                              aGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtcatca                >  1:1493473/1‑99 (MQ=255)

                                                                              aGTAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtcatca                >  1:2041878/1‑99 (MQ=255)

                                                                                tAGAGAGAGATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAAc              <  2:912407/99‑1 (MQ=255)

                                                                                 agagagagATCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACa             <  1:373012/99‑1 (MQ=255)

                                                                                  gagagagaTCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACAt            >  1:191897/1‑99 (MQ=255)

                                                                                  gagagagaTCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACAt            >  2:1218317/1‑99 (MQ=255)

                                                                                  gagagagaTCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACAt            >  2:78526/1‑99 (MQ=255)

                                                                                   agagagaTCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATa           <  1:326236/99‑1 (MQ=255)

                                                                                    gagagaTCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATAc          >  1:76623/1‑99 (MQ=255)

                                                                                    gagagaTCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATAc          >  1:1569603/1‑99 (MQ=255)

                                                                                     agagaTCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACg         <  1:1139394/99‑1 (MQ=255)

                                                                                       agaTCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCa       <  2:1321700/99‑1 (MQ=255)

                                                                                       agaTCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCa       >  1:433125/1‑99 (MQ=255)

                                                                                        gaTCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCAc      >  2:303512/1‑99 (MQ=255)

                                                                                        gaTCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCAc      <  2:2808925/99‑1 (MQ=255)

                                                                                         aTCGCCTGCTCACGGTAGATATCCCGACGGAATGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTGGTCGGCCAGCAACATACGCAcc     >  2:52102/1‑98 (MQ=255)

                                                                                           ccccTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTc   >  1:651592/1‑99 (MQ=255)

                                                                                            cccTGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCg  >  2:2435044/1‑99 (MQ=255)

GTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCG  >  NC_000913/3535523‑3535713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A