Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F60 I1 R1
|
23 |
39.0 |
1343410 |
93.4% |
1254744 |
145.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913_3_hsa_tpiA |
2,106,741 |
A→T |
S162S (TCT→TCA) |
wbbH ← |
O‑antigen polymerase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913_3_hsa_tpiA | 2,106,741 | 0 | A | T | 100.0%
| 111.0
/ NA
| 34 | S162S (TCT→TCA) | wbbH | O‑antigen polymerase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (12/22); total (12/22) |
TCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTAACC > NC_000913_3_hsa_tpiA/2106597‑2106883
|
tctATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGAcc > 2:72272/1‑149 (MQ=255)
gCATAAGAGAACATAACAATAAAGAAGATGTCCCTACCAGTATTTATGATAATTGCAAAGATGAATACAATAAAAACCAGTATAGTAAATTTTTTACTTACCGTTGTATTAGTAAATTTCTGTGATCAAATCAATAAAGCAAAagtagt < 2:315192/149‑1 (MQ=255)
atatgatAACCATAAAGACAATTTGCGTACAAGAATTCAGTATAATTGCAAAGATAAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATg < 2:68130/147‑1 (MQ=255)
gatAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTg < 2:460194/149‑1 (MQ=255)
tAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATATATAAAGCAAAAGTAGTTAGAATGATTGGc > 1:576847/1‑149 (MQ=255)
aaCCATAAATACAATTTGCTTACCATGATTGAGTATAATTGCAAAGATGAATAAAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCt < 2:186387/149‑1 (MQ=255)
aaaaCAATTTGCTTACCAGTATTTAGTATAATTGAAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTa < 2:168189/146‑1 (MQ=255)
aaGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAATAAAAATTAATTAGAATGATTGGCTGCATTTa > 2:179662/1‑149 (MQ=255)
aGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAGAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTAt > 2:638294/1‑149 (MQ=255)
ttGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTgaga < 1:489116/149‑1 (MQ=255)
gCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGaaa > 1:636125/1‑149 (MQ=255)
cTTACAAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAAt < 2:42231/149‑1 (MQ=255)
aCCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTc < 2:636125/149‑1 (MQ=255)
tATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTTCATGTATGCTGAGAAATTTCTTGAt > 1:413719/1‑149 (MQ=255)
ataATTCCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAATTAAATAAAGCAAAAGTAGTTGGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTc < 1:606808/149‑1 (MQ=255)
aGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCa > 1:408961/1‑149 (MQ=255)
aaTACAATAAAAACAAGTAGAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATctct < 1:638294/149‑1 (MQ=255)
tACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTa < 2:63415/149‑1 (MQ=255)
aCAATAAAAACAAGTAAAGTAAATGGTTTACTTACCTTTGTATTCGCCATTTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTAt < 1:153893/149‑1 (MQ=255)
aTAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTCTTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCaa > 1:600149/1‑149 (MQ=255)
aTAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCaa < 2:408961/149‑1 (MQ=255)
tAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCaaa < 2:8454/149‑1 (MQ=255)
tAAAAACAAATAAAGTAAAGATTTAACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAACCTATATCGCTGTCTTCAACATCAGCATCTCTTATCaaa < 2:482499/149‑1 (MQ=255)
aagtaaagttAATGTTTTACTTAACTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCACCATCTCTTATCAAATTCatat < 2:591336/149‑1 (MQ=255)
agtaaaTGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCtaa < 2:600149/149‑1 (MQ=255)
agtaaaTGTTTTACTTACCTTGTTATTAATAACTTTTTTTGACCAGAAAAGAAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCtaa < 2:71935/149‑1 (MQ=255)
gtaaaTGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGATCTATTAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTTAGAAATTTCTTCATGTGTCTTCAACATCAGCATCTCTTATCACATTCAAATATCtact > 2:325866/1‑147 (MQ=255)
taaaTGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCtaatt < 1:34602/149‑3 (MQ=255)
aaTGTTTTACTTACCTGTGTAGTAGAAAATATTTTTGACCAAATGAATAAAGCAAAAGTAGTTAAAATGATTGGCTGCATGTGTGCTGAAAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCttagtca > 2:518767/1‑147 (MQ=255)
tGTTTTACTAAACTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGc < 2:15226/149‑1 (MQ=255)
cTTTGTATTAGTAAATTTTTTTTTTTTATTAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAAATTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATTTATCTAATTAAGCTAGTCCCGAACt > 2:552797/1‑149 (MQ=255)
ttGTATTAGTAAATTTTTTTGACCAAATACATAAAGAAAACGGAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTgg < 1:461621/149‑1 (MQ=255)
agtAGTAAATTTTTTTGAACAGATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGt < 2:656059/147‑1 (MQ=255)
ttttttTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTCTGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTAccc > 1:151842/1‑149 (MQ=255)
|
TCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTAACC > NC_000913_3_hsa_tpiA/2106597‑2106883
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
