Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F21 I1 R1	17	37.6	1357466	94.5%	1282805	141.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	2,106,741	A→T	S162S (TCT→TCA)	wbbH ←	O‑antigen polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	2,106,741	0	A	T	100.0%	89.3 / NA	28	S162S (TCT→TCA)	wbbH	O‑antigen polymerase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (15/13); total (15/13)

CTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTT  >  NC_000913_3_hsa_tpiA/2106596‑2106879

ctctATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGAc                                                                                                                                         <  1:219451/149‑1 (MQ=255)

         cacCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATACAAACACCGCATTTTGACGGGTTACTTGCCTTTGTATTAGTAAGTTTTTTTTACCaattaaat                                                                                                                                >  1:19893/1‑149 (MQ=255)

            cTACGATGAATGCATAAGAAATGATAACCATACAGACAATTTTCTTACCAGTATTCAGTATAATTGCAGGGATAAATAGCATAAATACAAGTAAAGTAAATGTAGTGCTAACCTTTGTATTAGAAAATTTTTTTTACAAAAtaattaaa                                                                                                                             >  2:538228/1‑149 (MQ=255)

             tACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAACGTAAATGTTTTACTTACATTTGTCCCAGTAAATTTAGATGAGCAAAtaaataaac                                                                                                                            >  1:630626/1‑148 (MQ=255)

                         ctAAGAGATGATAACCATAAATCCAATTTCCTTACCAGTATTCAGTATAATTGGAAATATGAATACAATAAAAACAAGTAAATGAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTa                                                                                                                <  2:128938/148‑1 (MQ=255)

                         aTAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGCCTAAATAACTAAACCAAAAGTAATTa                                                                                                                >  2:170656/1‑149 (MQ=255)

                          tAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAg                                                                                                               >  1:68400/1‑149 (MQ=255)

                                       ccATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGc                                                                                                  >  1:615506/1‑149 (MQ=255)

                                              gACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTTTTTTTTTATATAAAGCAAAAGTAGTAAGAATGATTGCCTGCTTGTATg                                                                                           >  2:411851/1‑149 (MQ=255)

                                               aCAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGc                                                                                          <  1:487199/149‑1 (MQ=255)

                                                  atgggCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAAAGTTTTACTTACCTTTGTATTAATAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTga                                                                                       <  1:191588/145‑1 (MQ=255)

                                                        ttACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAAtt                                                                                 >  2:372438/1‑149 (MQ=255)

                                                           ccAGGATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATAATTGGCTGCATGTATGCTGAGAAATTTCt                                                                              <  1:574666/149‑1 (MQ=255)

                                                                 ttCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGAtgt                                                                        <  1:449112/149‑1 (MQ=255)

                                                                  tCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGAtgtg                                                                       >  1:607702/1‑149 (MQ=255)

                                                                          aTTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATATATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAAc                                                               <  1:500952/149‑1 (MQ=255)

                                                                              cAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCa                                                           <  1:92357/149‑1 (MQ=255)

                                                                                         aCAATAAAAACAAGTAAAATAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAATCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTAt                                                <  2:473152/149‑1 (MQ=255)

                                                                                                          agtaaaTGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCtaa                               <  2:68400/149‑1 (MQ=255)

                                                                                                             aaactttttACTTACCCTTGTATTAGTCAATTTTTTTGACAAAACAAATAACGCAGAAGTAGTTAGAATGATTGGCTGCATGGATGCTGAGAAATTTCTTGATGTGTCGTCAACATCAGCATCTCTTATCAAATTCATATAGCtgagta                            <  2:348086/144‑1 (MQ=255)

                                                                                                                tGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGc                         <  1:372438/149‑1 (MQ=255)

                                                                                                                  ttttACTTACCTTTGTAATAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATTGTCAAGTAAGCTa                       >  2:233253/1‑149 (MQ=255)

                                                                                                                            cTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAGGTAGTTAGAATGATTGGCTGAATGTATGCTGACAAAGTTCTTGATGTGTCTTCAAAATCAGCATCTCTTATCAAATTCATATAGCTTATTAAGCTAGTCCTGAACt             >  2:450473/1‑149 (MQ=255)

                                                                                                                            cTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACt             >  1:584300/1‑149 (MQ=255)

                                                                                                                                       tAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGAtgt                                                                        >  1:482634/1‑79 (MQ=255)

                                                                                                                                       tAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGAtgt                                                                        <  2:482634/79‑1 (MQ=255)

                                                                                                                                       tAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACtt  >  2:143343/1‑149 (MQ=255)

                                                                                                                                       tAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGGCTAATTATTAAATTCTTGATTTGTCTTCAATCTCTTAACCGCGGATCAAATGCATATAGCTAAGTAAGCTTATTCCGAACTGGTAGTTACtt  >  2:651882/1‑149 (MQ=255)

CTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTT  >  NC_000913_3_hsa_tpiA/2106596‑2106879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A