Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F29 I1 R1	20	72.4	2486344	94.1%	2339649	144.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	2,106,741	A→T	S162S (TCT→TCA)	wbbH ←	O‑antigen polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	2,106,741	0	A	T	98.8%	271.9 / ‑5.2	82	S162S (TCT→TCA)	wbbH	O‑antigen polymerase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base T (41/40); total (42/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

ACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTAGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTAACCTCA  >  NC_000913_3_hsa_tpiA/2106595‑2106886

aCTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGa                                                                                                                                                 >  1:470393/1‑149 (MQ=255)

    tATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGGATTCAGTATAATTGCAAAGATGAATACACACAGTACAATTAAAGTAAATGTTTTACTTACACTTGTATTAGTA‑AATTTTTTTGAACaa                                                                                                                                             >  1:602673/1‑149 (MQ=255)

     aTTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCaaa                                                                                                                                            <  1:1083141/149‑1 (MQ=255)

        aaCACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTCGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCaaataa                                                                                                                                         >  1:1013950/1‑149 (MQ=255)

            cgtACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAAGTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAataaataa                                                                                                                                     <  2:27697/147‑1 (MQ=255)

             cTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATTAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAAtaaataaa                                                                                                                                    >  1:578752/1‑149 (MQ=255)

             cTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAAtaaataaa                                                                                                                                    <  2:475805/149‑1 (MQ=255)

             cTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAAtaaataaa                                                                                                                                    >  2:375674/1‑149 (MQ=255)

              tACGATGAATGCATAAGTGATGATAACAATAACCACAATGCGCTTCCCAGTATTCATTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGCCCAaatacatatat                                                                                                                                   >  2:268897/1‑146 (MQ=255)

              tACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGAATAATTGCAAAGATGAATACAATAAAAACAAGTCAAAACATTGTGTTACTTACTTTTGTATTAGTA‑AATTTTTTTGACCAAGAAAATAAAg                                                                                                                                   >  1:1099188/1‑149 (MQ=255)

              aaCGATGAATGCATAAGAGATGAAAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAg                                                                                                                                   <  2:657263/148‑1 (MQ=255)

                   tGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCaaaa                                                                                                                              <  1:207149/149‑1 (MQ=255)

                    gAATGCATAAGAGACGATAACCATAAAGACAATTTACTTACCAGTATTCAGTATACTTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCATCGTATTCGTA‑ATTTTTTTTGACAACATAAGTAAAGCaaact                                                                                                                             >  2:617625/1‑147 (MQ=255)

                          atcagagATGATAACCATAAAGACAACTTGCTTACCAGTATTCAGTATAATTGCAAACATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑CATTTTTTTGACTAAATAAATAAACCAAAcgttgtaa                                                                                                                       >  2:539949/4‑147 (MQ=255)

                           tAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACAAAATAAATAAAGCAAAAGAAGATAg                                                                                                                      >  2:241781/1‑149 (MQ=255)

                            aaGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTCTACGTACCGTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGa                                                                                                                     >  1:912584/1‑149 (MQ=255)

                             agagATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGaa                                                                                                                    <  2:116815/149‑1 (MQ=255)

                             agaaaagatAAACAGAAAGACAATTTAATTACCAGTATTCAGTATAAATGAAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACGTACCTTTGTATTAGTA‑AATTTTTGTGACCGGATAAATAAAGCAAAAGTAGTTAGaa                                                                                                                    <  2:183035/143‑1 (MQ=255)

                              gagaTGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAAt                                                                                                                   <  1:257109/149‑1 (MQ=255)

                              gagaTGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAAt                                                                                                                   >  1:922167/1‑149 (MQ=255)

                                 actatAACCAAAAAGACTAGTTGCTTACCAGTACTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGAt                                                                                                                <  2:746967/146‑1 (MQ=255)

                                  tcatAACCATAAACACAATTTACTTACCAGTAGGCAGAATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGAACCACTTTGTAGATC‑TAGGAATCAAGCAAAAGTAGTTAGAATGAtt                                                                                                               >  2:259516/3‑149 (MQ=255)

                                    agaaCCATAAAGAAAATTAGTTTACGTGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGGAAAGTAAATGATTTACTTACCTTTGTATTAGTA‑AATTTTTTTGAGCCGATGAATAAAGCAAAAGTAGTTAGAATGATTgg                                                                                                             <  2:894198/147‑1 (MQ=255)

                                     aaaCCATAAAGAAAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGc                                                                                                            <  2:101609/148‑1 (MQ=255)

                                        ccATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTtgctgc                                                                                                         >  2:1240594/1‑149 (MQ=255)

                                         cATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAAAATGATTGACTGCa                                                                                                        >  1:723311/1‑149 (MQ=255)

                                          aTAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCAt                                                                                                       <  1:1024156/149‑1 (MQ=255)

                                                  aaTTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTg                                                                                               >  1:518246/1‑149 (MQ=255)

                                                  aaTTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTg                                                                                               >  1:850072/1‑149 (MQ=255)

                                                         ttACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAAtt                                                                                        >  1:478507/1‑149 (MQ=255)

                                                          tACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAAttt                                                                                       <  1:355814/149‑1 (MQ=255)

                                                          tACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAAttt                                                                                       <  2:597612/149‑1 (MQ=255)

                                                          tACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAAttt                                                                                       <  1:980814/149‑1 (MQ=255)

                                                            caaGTATTCAGGATAATTGCAAAGAGGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCt                                                                                     <  2:94296/147‑1 (MQ=255)

                                                             cAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAACGTAAATGTTTTACTTACCTTTGTCTTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAAATTCtt                                                                                    >  1:269473/1‑149 (MQ=255)

                                                             cAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAACGTAATTAGAATGATTGGCTGACTGTAGTCTAAGaattctttt                                                                                    >  2:140818/1‑142 (MQ=255)

                                                             cAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCtt                                                                                    >  1:645129/1‑149 (MQ=255)

                                                             cAGTATTCAATATAATTGCAAAGATTAATACAATAAAAACAAGTAAATTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAATTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCtt                                                                                    <  2:872704/149‑1 (MQ=255)

                                                              agtagtCAGTATAATTACAAAGATGAATACAATAAAAGCAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑ATTTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCttt                                                                                   <  2:1148477/149‑2 (MQ=255)

                                                                tATTCAGTATAATTGTAAAAATTAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGAt                                                                                 <  2:420207/149‑1 (MQ=255)

                                                                tATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGAt                                                                                 <  2:902211/149‑1 (MQ=255)

                                                                     aGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGCAGGATTGGCTGCATGTATGCTGAGAAATTTCTTGCTGTTTc                                                                            >  1:335426/1‑149 (MQ=255)

                                                                            ttGCTAAGATGAATACAATAAAATCAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑CATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACa                                                                     <  1:360627/149‑1 (MQ=255)

                                                                            ttGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACa                                                                     >  2:935410/1‑149 (MQ=255)

                                                                             tGCAAAGATGAATACAATAAAAAAAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACAt                                                                    <  2:243566/149‑1 (MQ=255)

                                                                               cAAAGAAGAATACAAAAAAAACAAGGAAAGTAAATGTTTCACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGGTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCa                                                                  <  2:615705/149‑1 (MQ=255)

                                                                                aaaGATGAATACAATAAAAACAAGTAAAGTAACTGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTTGCTGCATTTATGCTTATAAATTTCTTCATGTTTTTTCAACATCAg                                                                 >  2:610068/1‑149 (MQ=255)

                                                                                 aaGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGc                                                                >  1:158690/1‑149 (MQ=255)

                                                                                      gAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTTTTTTATTTAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTGGATGTGTCTTCAACTTCAGCAtctc                                                           >  2:86237/1‑149 (MQ=255)

                                                                                      gAATAAAATAGAAAAAAGTAAAGTAAATGTTTTACTTACTGTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCAtctc                                                           <  2:1140447/149‑1 (MQ=255)

                                                                                         tACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTATTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTa                                                        <  1:375674/149‑1 (MQ=255)

                                                                                         tACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTa                                                        >  2:563072/1‑149 (MQ=255)

                                                                                          aCAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATACTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTAt                                                       >  2:94474/1‑149 (MQ=255)

                                                                                            aaTAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCa                                                     >  2:1076116/1‑149 (MQ=255)

                                                                                             aTAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTTTTTTTTTTTTTAAGGCATAATTATATAGAATGATTGGCTGCTTGTTTGCTGAGAAAATTCTTGATGTGTCTTCAACATAAGCATCTCTTATCaa                                                    >  2:824313/1‑149 (MQ=255)

                                                                                              tAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCaaa                                                   >  1:524411/1‑149 (MQ=255)

                                                                                                aaaaCAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAAtt                                                 <  2:138627/149‑1 (MQ=255)

                                                                                                        taaagtaaaTGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAGTTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCTTCTAGc                                         >  2:594579/1‑149 (MQ=255)

                                                                                                             taaaTGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCtaagt                                    >  2:488111/1‑149 (MQ=255)

                                                                                                             taaaTGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCtaagt                                    >  1:734119/1‑149 (MQ=255)

                                                                                                                aTGTGTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCtaagtaag                                 <  2:765890/149‑1 (MQ=255)

                                                                                                                  gTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTa                                                                                                                       <  2:987991/61‑1 (MQ=255)

                                                                                                                  gTTTTACTTACCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTa                                                                                                                       >  1:987991/1‑61 (MQ=255)

                                                                                                                       acttacCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTcc                          <  2:602673/149‑1 (MQ=255)

                                                                                                                         ttacCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGAt                                                                                 >  1:803343/1‑92 (MQ=255)

                                                                                                                         ttacCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGAt                                                                                 <  2:803343/92‑1 (MQ=255)

                                                                                                                           acCTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGaa                      <  1:631733/149‑1 (MQ=255)

                                                                                                                             cTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACt                    >  2:552887/1‑149 (MQ=255)

                                                                                                                             cTTTGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACt                    >  2:545614/1‑149 (MQ=255)

                                                                                                                              tttGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTg                   <  2:461286/149‑1 (MQ=255)

                                                                                                                              tttGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTg                   >  2:609459/1‑149 (MQ=255)

                                                                                                                               ttGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTgg                  <  2:734119/149‑1 (MQ=255)

                                                                                                                                tGTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGt                 <  1:994615/149‑1 (MQ=255)

                                                                                                                                 gTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTa                >  1:325105/1‑149 (MQ=255)

                                                                                                                                 gTATTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTa                <  1:820061/149‑1 (MQ=255)

                                                                                                                                   aTTAGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGt              >  1:1152120/1‑149 (MQ=255)

                                                                                                                                      aGTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTTTGAGCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTAc           <  1:609459/149‑1 (MQ=255)

                                                                                                                                       gTA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACt          >  2:411176/1‑149 (MQ=255)

                                                                                                                                        tA‑AATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACtt         <  1:1192156/149‑1 (MQ=255)

                                                                                                                                         a‑aaTTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTa        <  2:850072/149‑1 (MQ=255)

                                                                                                                                            aTTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTAAc      <  1:1076116/149‑1 (MQ=255)

                                                                                                                                               ttttTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTTTCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTAACCTc   <  1:814455/149‑1 (MQ=255)

                                                                                                                                                ttttGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTAACCTCa  <  1:241781/149‑1 (MQ=255)

ACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTA‑AATTTTTTAGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTAACCTCA  >  NC_000913_3_hsa_tpiA/2106595‑2106886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A