Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F22 I1 R1 13 46.3 1618126 93.8% 1517802 142.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_bme_pgi 4,122,984 C→T R119H (CGT→CAT)  ftsN ← essential cell division protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi4,122,9840CT100.0% 182.5 / NA 55R119H (CGT→CAT) ftsNessential cell division protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/27);  total (28/27)

GGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGCGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGT  >  NC_000913_3_bme_pgi/4122838‑4123129
                                                                                                                                                  |                                                                                                                                                 
ggCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGCGTTTGCTGCCGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAACAAGCTGATgt                                                                                                                                                 >  2:474983/1‑149 (MQ=255)
    gtGCTGTTCCGCTAATTGCTGCGCGTGACGTTGGGGCTGTAGGGTTTGCTGACGCTGTTCCGGCGTCTGTTCACTCCACGGAACTTCAACCAGCTGCGTTTGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATgttgtt                                                                                                                                             <  2:235477/148‑1 (MQ=255)
      gCTGTTCCGCTAATTGCTGCGCCTGATGTTGGCGCTGTAGCGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCt                                                                                                                                           <  2:786098/149‑1 (MQ=255)
      gCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGCGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCt                                                                                                                                           >  1:33037/1‑149 (MQ=255)
       cTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGCGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTg                                                                                                                                          <  1:567999/149‑1 (MQ=255)
       cTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGCGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTg                                                                                                                                          >  1:748158/1‑149 (MQ=255)
         ggtCCGCTAATTGCTGTCCGGGACGTTGGCGCTGTAGCGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGgt                                                                                                                                        <  2:221233/147‑1 (MQ=255)
         gTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGCGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGgt                                                                                                                                        >  2:429632/1‑149 (MQ=255)
              gcttcttGCTGCGCCTCACGTTGGCGTTGTAGCGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCAGATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAg                                                                                                                                   <  1:780467/144‑1 (MQ=255)
              gCTAATTGCTGCGCCTGACGTTGGCGCTGTAGCGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAGGCTGATGTTGTTCTAgtgtgct                                                                                                                                   >  2:607521/1‑146 (MQ=255)
              gCTAATTGCTGCGCCTGACGTTGGCGCTGTAGCGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTCGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAg                                                                                                                                   >  2:50489/1‑149 (MQ=255)
                tAAGTGCTGCGCCTGACGTTGTCGCTGTAGCGTTTGCTGGCGCTGTTCCGGGGTCTGTTCATTCCACGGCACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCTGCCTGCATTTGTTTAAGAAGCTGATGTTGTTCTGGTGTCAGtt                                                                                                                                 <  2:52298/149‑1 (MQ=255)
                  aTTGCTGCGCCTGACGTTGGCGCTATAGCGATTGATGACGCTGTTCCGGCGTCTCTCCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCCGCCTGCATTTGTTCAAGCAGCTGATTTTGTTCCGTTGACAGtttc                                                                                                                               >  2:519300/1‑147 (MQ=255)
                     gctgcGCCTGACGTTGGCGCTGTAGCGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTcc                                                                                                                            >  2:352668/1‑149 (MQ=255)
                       tgcGCCTGACGTTGGCGCTGTAGCGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATGAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTGGCTCCgg                                                                                                                          >  1:4510/1‑149 (MQ=255)
                       tgcGCCTGACGTTGGCGCTGTAGCGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCgg                                                                                                                          >  1:485179/1‑149 (MQ=255)
                        gcgcCTGACGTTGGCGCTGTAGCGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGc                                                                                                                         <  1:656961/149‑1 (MQ=255)
                           ccTGACGTTGGCGCTGTAGCGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGtt                                                                                                                      <  1:338365/149‑1 (MQ=255)
                                cGTTGGCGCTGTAGCGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCTAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTttcac                                                                                                                 <  2:741616/149‑1 (MQ=255)
                                cGTTGGCGCTGTAGCGTGTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGGTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTttcac                                                                                                                 <  1:309392/149‑1 (MQ=255)
                                cGTTGGCGCTGTAGCGTCTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTttcac                                                                                                                 <  1:473973/149‑1 (MQ=255)
                                                tttGCTGACGCTGTTCCGGTGTCTGTTCATGCCACGGAACTTCAACCAGCTGTGTTGGCTGCTGGCGCATATCGGCGTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGaa                                                                                                 <  2:124312/149‑1 (MQ=255)
                                                tttGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGaa                                                                                                 <  1:352668/149‑1 (MQ=255)
                                                          cTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGATTCTGTgg                                                                                       <  2:706637/149‑1 (MQ=255)
                                                            gTTCCGGCGTCTGTGCATTCTACGGATCTTTAACCAGGTGCGTTGGCTGTTGGCGCATATCAGCCTGAATTTGTTCAAGAAGTTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGgc                                                                                     <  2:105878/149‑1 (MQ=255)
                                                                    gTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCgcacgcac                                                                             >  2:418835/1‑149 (MQ=255)
                                                                    gTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCgcacgcac                                                                             >  1:276567/1‑149 (MQ=255)
                                                                     tCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCaccacc                                                                                                        <  2:233451/121‑1 (MQ=255)
                                                                     tCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCaccacc                                                                                                        >  1:233451/1‑121 (MQ=255)
                                                                     tCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACt                                                                            >  1:94091/1‑149 (MQ=255)
                                                                          tCATTCCACGGAGCTGCAACGCGGTCCGTTGGGTGTTGGCGCATATCGGCCTGCATTTGTTCAGGAAGCTGATGTTGTTCGGGTGTCAGTTGCCCCGGGGTTTTCATTTCACCACCGGCAGAAGGTTCTGTGGAcgcaggcattgttgg                                                                       <  2:97975/149‑8 (MQ=255)
                                                                             ttCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTg                                                                    <  2:748158/149‑1 (MQ=255)
                                                                             ttCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTCCTCCGGCGTTTTGACTTCACCACCTGCAGAAGGTTCTGTGGGCGCTCGTCCTCCCGGCtt                                                                    >  2:657756/1‑148 (MQ=255)
                                                                              tCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTgg                                                                   <  1:307572/149‑1 (MQ=255)
                                                                              tCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTgg                                                                   <  1:58517/149‑1 (MQ=255)
                                                                                cACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCg                                                                 >  2:745914/1‑149 (MQ=255)
                                                                                 aCGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGttt                                                                                                                     >  2:554333/1‑96 (MQ=255)
                                                                                 aCGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGttt                                                                                                                     <  1:554333/96‑1 (MQ=255)
                                                                                 aCGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACCCCCGGCTGGCGa                                                                >  2:468198/1‑149 (MQ=255)
                                                                                     gaCTTCAACGAGCTGCGTTAGCTTCCGGCGAATCTCAGCCTGCATTTGTTCAAGTATCTGATGTTGTTCTGGTGTCAGTTGCGCCGTCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACttt                                                            <  2:563944/148‑1 (MQ=255)
                                                                                        ttCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCa                                                         >  1:547215/1‑149 (MQ=255)
                                                                                              cAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCCATCCTGGCTGGAGAGTTTCCGGCTCtt                                                   >  1:236237/1‑149 (MQ=255)
                                                                                                             ctgGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGc                                    <  1:429632/149‑1 (MQ=255)
                                                                                                             ctgGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCCCCAGc                                    >  2:47343/1‑149 (MQ=255)
                                                                                                              tgGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTccc                                                                         >  1:164510/1‑111 (MQ=255)
                                                                                                              tgGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTccc                                                                         <  2:164510/111‑1 (MQ=255)
                                                                                                                gcgcATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGtt                                 >  1:152836/1‑149 (MQ=255)
                                                                                                                    atatCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGttcttc                             <  1:745914/149‑1 (MQ=255)
                                                                                                                    atatCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGttcttc                             <  1:339798/149‑1 (MQ=255)
                                                                                                                         aGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGtt                        >  1:200693/1‑149 (MQ=255)
                                                                                                                                aTTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTtggtgg                 >  2:394899/1‑149 (MQ=255)
                                                                                                                                        aagaagCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCCGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGt         >  1:52731/1‑149 (MQ=255)
                                                                                                                                           aagCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTc      <  1:409514/149‑1 (MQ=255)
                                                                                                                                           aagCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTACCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTc      >  1:698275/1‑149 (MQ=255)
                                                                                                                                               tGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGt  >  1:561616/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGCGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGT  >  NC_000913_3_bme_pgi/4122838‑4123129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A