Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F41 I1 R1
|
15 |
48.1 |
1673764 |
93.6% |
1566643 |
143.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913_3_bme_pgi |
4,122,984 |
C→T |
R119H (CGT→CAT) |
ftsN ← |
essential cell division protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913_3_bme_pgi | 4,122,984 | 0 | C | T | 90.7%
| 113.3
/ 0.2
| 43 | R119H (CGT→CAT) | ftsN | essential cell division protein |
| Reads supporting (aligned to +/- strand): ref base C (3/1); new base T (19/20); total (22/21) |
| Fisher's exact test for biased strand distribution p-value = 6.07e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGCGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGT > NC_000913_3_bme_pgi/4122846‑4123122
|
tGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGCGTTTGCTGACGCTGTTCCGGCGCCTGTTCTTTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTgg > 1:370757/1‑149 (MQ=255)
ttCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGCGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGgtg > 1:298774/1‑149 (MQ=255)
tgagtgctggGCCTCACGTTGGCGCTGTAGCGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGTATACCAGCCTGGATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGtt < 2:827764/145‑1 (MQ=255)
tgcGCCTGACGTTGGCGCTGTAGCGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCgg > 1:69273/1‑149 (MQ=255)
aCGTTGGCGCTGTAGCGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTttca > 1:717844/1‑149 (MQ=255)
tGGCGCTGTAGCGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTttcacttc < 1:509671/149‑1 (MQ=255)
cTGTAGCGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTcaccac < 1:442444/149‑1 (MQ=255)
tgccGCTGCTCCGGCGTCTGCTCATTCCACGGAACTTCAACCAGCCGCGGTGGCTGCTGTCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTc < 2:717844/146‑1 (MQ=255)
aCGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGTTGGCGCATATCAGCCTGCATTTGTTCAGGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTGCGTTCTCTCCTGGCAGAAGTTTCtg > 2:249346/1‑149 (MQ=255)
gCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCTCCACCGGCAGAAGGTCCcgtg > 2:373745/1‑149 (MQ=255)
gCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCt > 2:451437/1‑146 (MQ=255)
gCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCt < 1:451437/146‑1 (MQ=255)
gCTGTTCCGGCGGCTGTTAATTCCACAGAACTTCAATCAGCTGCGTTGGCTGCTTGTTCATATCAGGCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCtgtg < 1:602879/149‑1 (MQ=255)
cTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTcacca < 1:731279/130‑1 (MQ=255)
cTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTcacca > 2:731279/1‑130 (MQ=255)
tGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGGGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTTTggg > 2:597789/1‑149 (MQ=255)
cGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATGAGCCTGCATTTGTTCAAGAAGCTGACGTTTTTCTGGTCTCATTCGCGCCGGCGTTTTCACTTCACCGCCGGCGGACGGTTCTGTGGGcgcac > 1:323684/1‑149 (MQ=255)
ggCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGcgcacg > 2:530640/1‑149 (MQ=255)
tGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGCTGTTGTTCTGGTGTCAGTTGCGCCGGCGTTTTCACTTCACCACCGCCAGAAGGTGCTGTTGACGCActccctcc > 2:10870/1‑149 (MQ=255)
ttCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCCCGCACTCCCg > 1:596710/1‑149 (MQ=255)
cATCCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGc < 1:346993/149‑1 (MQ=255)
ttCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTg > 1:432913/1‑149 (MQ=255)
aCGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCCCCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGa < 1:664788/149‑1 (MQ=255)
aCGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGa > 2:522458/1‑149 (MQ=255)
tCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAg < 2:432913/149‑1 (MQ=255)
aaCCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGCGCGCACGCACTCCCGGCTGGCGACTTTCCAGct > 2:482429/1‑149 (MQ=255)
ttGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAg < 1:126935/149‑1 (MQ=255)
tGGCTGCTGGCGCATATCATCATGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAgc < 1:101732/149‑1 (MQ=255)
tGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAgc > 2:288637/1‑149 (MQ=255)
ggCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGCTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGttttg < 2:770618/149‑5 (MQ=255)
tgctgGCGCATATCAGCCTGCATTTGTTCAAGAAGATGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGCCTGGCGACTTTCCAGCTCTTTATCGTAGCGCCa > 2:516945/1‑149 (MQ=255)
gcATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCaccacc < 2:373564/76‑1 (MQ=255)
gcATATCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCaccacc > 1:373564/1‑76 (MQ=255)
tatCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCg > 2:418576/1‑74 (MQ=255)
tatCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCg < 1:418576/74‑1 (MQ=255)
tatCAGCCTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTtcttct < 1:392312/149‑1 (MQ=255)
tatCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTtcttct < 1:530640/149‑1 (MQ=255)
cTGCATTTGTTCAAGAAGCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCTCGCGCTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTtg < 1:373745/149‑1 (MQ=255)
gCATTTGTTCAAGAAGCTGATGTTGTTTTGGTGTCTGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGGAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTTATGTAGCGCCAGCGTTCTTCTGGTTTtggt < 1:767686/149‑1 (MQ=255)
taTTTGTTTAAGAAGCTGATGGTGTTCTGGTGTCAGTTGCACCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTtggtg < 1:795505/148‑1 (MQ=255)
aagaagCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACGGGGGTCGGAGTGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGt > 2:557449/1‑149 (MQ=255)
gaagCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGtttt > 2:37065/1‑134 (MQ=255)
gaagCTGATGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGtttt < 1:37065/134‑1 (MQ=255)
|
TGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGCGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGT > NC_000913_3_bme_pgi/4122846‑4123122
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
