Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F19 I0 R1	34	56.7	1876034	94.8%	1778480	147.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913_3_pae_tpiA	3,560,455:1	+G	100%	intergenic (‑2/+1)	glpR ← / ← glpR	pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	3,560,455	1	.	G	96.6%	184.1 / ‑1.3	58	intergenic (‑2/+1)	glpR/glpR	pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (15/40); total (17/41)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTT  >  NC_000913_3_pae_tpiA/3560307‑3560597

gCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTGCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑c                                                                                                                                                 <  1:436176/149‑1 (MQ=255)

  cGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑cgc                                                                                                                                               <  2:917739/149‑3 (MQ=255)

   gATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAGAGCAGCGCCGCACCGCCAT‑GATGG‑cgca                                                                                                                                              >  1:11193/1‑146 (MQ=255)

    atatCGATAATCCGCGTCGAGCCATTCGGGACTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑cgcag                                                                                                                                             <  2:494994/149‑5 (MQ=255)

     tatCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑cgcagg                                                                                                                                            <  1:900475/149‑6 (MQ=255)

         gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCa                                                                                                                                        <  1:639874/149‑1 (MQ=255)

             aaCAGCGTCGAGACAATGAGTATTAGCTCCGACACTGTGCGGGCGATGCGCTCTTTTTCTTAGATCTGGGTGGCCTTGCGATCGAGCCACGGCGTGTTAACCGAACAGAAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGtt                                                                                                                                    <  2:204836/149‑1 (MQ=255)

              aCAGCGTCGAGCCATTGGGGAGTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGttt                                                                                                                                   <  1:768880/149‑1 (MQ=255)

                aGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACCTGACGGCAGCGCCACACCGCCATAAATGC‑C‑CAAGATCaccttatg                                                                                                                                 >  2:98025/1‑142 (MQ=255)

                 gtgTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGTAGGATCAGGTTTTGc                                                                                                                                <  2:496980/147‑1 (MQ=255)

                 gCGTCGAGCCATTGCGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGc                                                                                                                                <  1:756878/149‑1 (MQ=255)

                      gAGCCATGGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGc                                                                                                                           <  1:58368/149‑1 (MQ=255)

                       aGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGcc                                                                                                                          >  1:389809/1‑149 (MQ=255)

                          cATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGc                                                                                                                       <  2:296909/149‑1 (MQ=255)

                           aTTGGGGATTTGCTCCGCCACTTTGCGGGGGATGCGCGCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGACCTGGAAGGCAGCGCCGCGCCGCCAC‑GCTGG‑CGCAGGATCAGGTTGTGCGCCGCCAGCt                                                                                                                      >  1:79238/1‑149 (MQ=255)

                            ttGGGGATTTGCAACGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCAACGGCGTGTTAACCGAACTGGAAGGCAGCGCAGAACCACAAT‑GTTAG‑CACAGGCGCAGGTTGTGCTCCGACAGcac                                                                                                                     >  2:361289/1‑147 (MQ=255)

                                  aTTTGCTCCGTCACTTTGCGGGAGATGCGCTCTGTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAg                                                                                                               <  2:197759/149‑1 (MQ=255)

                                  aTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAg                                                                                                               <  2:390535/149‑1 (MQ=255)

                                      gCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcg                                                                                                           >  1:937142/1‑149 (MQ=255)

                                         ccGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcgg                                                                                                        <  1:333280/149‑1 (MQ=255)

                                            ccACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTTTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCCCCGCCAT‑GATGG‑CGCCAGATCAGGATTTGCTCCGCCAGCTCCTTGAGGTCGCGGCGa                                                                                                     >  2:726573/1‑149 (MQ=255)

                                               cgttGCGGGCGATGCGCCCTATTTCTTCACTCTGGGTACAACTGCCACCGTGCCACGTAGAATTAACACAGCTGCAAGACAGCGCCGCACCCCCAT‑AATCA‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATa                                                                                                  >  2:189826/3‑149 (MQ=255)

                                               cTTTGCGGGCGATGCGCTGTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATa                                                                                                  <  2:129122/149‑1 (MQ=255)

                                               cTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAACACCTG‑TAGGT‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATa                                                                                                  <  1:731639/149‑1 (MQ=255)

                                               cTTTGCGGGCGATGCGCTCTTTTTCTTCGGACTGGGTGGCCTTGCGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGTTTCGCCAGCTCATTGAGGTCGCGGCGAATa                                                                                                  <  1:691690/149‑1 (MQ=255)

                                                    cGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTg                                                                                             <  1:147190/149‑1 (MQ=255)

                                                     gggCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGc                                                                                            <  1:888934/149‑1 (MQ=255)

                                                           tGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCCGGATCAGGTTTTGCTCCGCCAGCTCATTGAGCTCGCGGCGAATAGTCTGCGGGcca                                                                                      >  2:695665/1‑147 (MQ=255)

                                                            gcgcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGa                                                                                     <  2:625020/149‑1 (MQ=255)

                                                             cgcTCTTTTTCTTCGTGCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGAc                                                                                    <  2:615829/149‑1 (MQ=255)

                                                              gcTCTGTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACg                                                                                   <  1:761141/149‑1 (MQ=255)

                                                                tctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAAACGAACTGGAAGTCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGga                                                                                 >  1:184958/1‑149 (MQ=255)

                                                                  tttttCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgaga                                                                               >  1:558864/1‑149 (MQ=255)

                                                                     ttcttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAAt                                                                            >  1:107023/1‑149 (MQ=255)

                                                                      tcttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATg                                                                           <  2:632556/149‑1 (MQ=255)

                                                                      gtttcGGTCTGGGTGGCCTTGCGAGCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATg                                                                           <  2:924794/147‑1 (MQ=255)

                                                                       cttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGc                                                                          <  1:413674/149‑1 (MQ=255)

                                                                            gTCTGGGTGGCCTGGCGATCGTGCCACGGCGTGTTAACCGGAGTGGATGGCAGCGCCTCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAc                                                                     <  2:200742/149‑1 (MQ=255)

                                                                            gTCTGGGTGGCCGTGCGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAc                                                                     <  2:629823/149‑1 (MQ=255)

                                                                             tCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGA‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAca                                                                    >  1:653085/1‑148 (MQ=255)

                                                                                 ggTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGACCGCTCATTGATGTCGTGGCGAAGTGGCGGCGGGCTGACGGAGAAATGCTCTACCAGct                                                                <  1:468275/149‑1 (MQ=255)

                                                                                 ggTGGCCTTGCGATAGAGGCACGGCGAGTAAACCGAACTCGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGct                                                                <  1:18919/149‑1 (MQ=255)

                                                                                   gtgCCGTGCTATCGTGCCATGGTGTTTTAACCGAACTGTAAGGCAGCGCCGCACCGCCAT‑GATGGAG‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGATGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGctct                                                              <  2:596591/147‑1 (MQ=255)

                                                                                      ccTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg                                                           <  2:144542/149‑1 (MQ=255)

                                                                                         tGCGATCGTGGCACGGCGTGTTAACTGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa                                                        <  1:90824/149‑1 (MQ=255)

                                                                                         tGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa                                                        <  1:554736/149‑1 (MQ=255)

                                                                                             aTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCCCCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCCCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGa                                                    >  2:222518/1‑149 (MQ=255)

                                                                                             aTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGa                                                    <  2:178366/149‑1 (MQ=255)

                                                                                              gcGTGCCACGGCGGGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGAc                                                   <  2:296381/148‑1 (MQ=255)

                                                                                                gTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg                                                           >  2:358455/1‑139 (MQ=255)

                                                                                                gTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg                                                           <  1:358455/139‑1 (MQ=255)

                                                                                                    cACGGCGTGTGAACCGAATTGGAAGGCAGCGCCGCACCGCCAT‑GATGT‑CGCAGGATCAGGTTTTGCTCCGTCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAcc                                             <  2:90033/149‑1 (MQ=255)

                                                                                                      cGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCTAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACAtttagct                                           <  2:814885/149‑7 (MQ=255)

                                                                                                       ggCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctg                                          <  1:817471/149‑1 (MQ=255)

                                                                                                        gCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgc                                         <  1:683690/149‑1 (MQ=255)

                                                                                                                  cGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCCTTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACCTAACCCTGCTGTTTAACCa                               >  1:854107/1‑149 (MQ=255)

                                                                                                                       tGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCg                          >  1:215671/1‑149 (MQ=255)

                                                                                                                          aaGGCAGCGCCGCACCGCCAT‑GATGG‑CGCATGAGCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATATTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGata                       <  1:434898/149‑1 (MQ=255)

                                                                                                                               aGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAcc                  <  2:558864/149‑1 (MQ=255)

                                                                                                                                  gctggcgcGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCGAGCTCATTGAGGTGGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAACCGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGtt               <  2:52838/142‑1 (MQ=255)

                                                                                                                                    cgcACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgt             <  1:507959/149‑1 (MQ=255)

                                                                                                                                         cGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGt        >  2:581592/1‑149 (MQ=255)

                                                                                                                                                gatgG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtt  <  2:742713/149‑1 (MQ=255)

GCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTT  >  NC_000913_3_pae_tpiA/3560307‑3560597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A