Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A22 F101 I1 R1 19 63.1 2283916 94.2% 2151448 141.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA3,560,4551.G98.2% 189.1 / ‑4.0 57intergenic (‑2/+1)glpR/glpRpseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base G (25/31);  total (26/31)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

GCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGC‑CAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGT  >  NC_000913_3_pae_tpiA/3560307‑3560594
                                                                                                                                                      |                                                                                                                                            
gCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGc                                                                                                                                               >  2:298614/1‑149 (MQ=255)
  cGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCCAACTGTAATGCAGCGCCGCACCGCCCTG‑ATGGc‑cc                                                                                                                                            >  2:536272/1‑148 (MQ=255)
  cGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGACACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCGATG‑ATGGcgc                                                                                                                                             >  1:439699/1‑147 (MQ=255)
   gATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCTGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGcgca                                                                                                                                            <  2:829747/149‑4 (MQ=255)
   gATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGcgca                                                                                                                                            <  2:829165/149‑4 (MQ=255)
         gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCa                                                                                                                                      <  1:350518/149‑1 (MQ=255)
          atacagaGCGTCGAGGCATTTGGGAGCTGGTCCGCGAGTTTGGGGCCGCGGCGCGCTTTTTCTGCGGGCTGGGGGGCCGTGCCATCTTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCTATG‑ATGGCGCAGGATCAg                                                                                                                                     <  2:410816/143‑1 (MQ=255)
          aTAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAg                                                                                                                                     >  2:126750/1‑149 (MQ=255)
          aTAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AGGGCGCAGGATccg                                                                                                                                     >  2:139000/1‑147 (MQ=255)
           tAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAgg                                                                                                                                    <  1:306767/149‑1 (MQ=255)
              aCAGCGTTGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGtt‑t                                                                                                                                <  1:682671/149‑1 (MQ=255)
              aCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGACGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGtt‑t                                                                                                                                <  1:960630/149‑1 (MQ=255)
              aCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGtt‑t                                                                                                                                >  2:643929/1‑149 (MQ=255)
                    tCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTcc                                                                                                                          <  1:643929/149‑1 (MQ=255)
                      gAGCCATTGGGGTTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGc                                                                                                                        <  2:691482/149‑1 (MQ=255)
                      gAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGACATTTCGTGCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGc                                                                                                                        >  1:503255/1‑149 (MQ=255)
                            ttGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTc                                                                                                                  >  1:708382/1‑149 (MQ=255)
                            ttGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTc                                                                                                                  >  1:728545/1‑149 (MQ=255)
                              ggggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCCCAt                                                                                                                >  2:946240/1‑149 (MQ=255)
                               gggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCAtt                                                                                                               <  1:597124/149‑1 (MQ=255)
                                 gATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCGCATTGa                                                                                                             >  1:284092/1‑149 (MQ=255)
                                  ctatGCGCCGCCAGTTTGCGGGGGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAg                                                                                                            <  2:780095/146‑1 (MQ=255)
                                   tttGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGTCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGCgg                                                                                                           >  2:1086700/1‑149 (MQ=255)
                                      gCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTcg                                                                                                        >  2:686783/1‑149 (MQ=255)
                                        tCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTcgcg                                                                                                      >  1:459764/1‑149 (MQ=255)
                                           gCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGAt                                                                                                                                        <  1:618944/113‑1 (MQ=255)
                                           gCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGAt                                                                                                                                        >  2:618944/1‑113 (MQ=255)
                                             cACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGaa                                                                                                 >  1:854054/1‑149 (MQ=255)
                                                    cGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTg                                                                                          <  1:720894/149‑1 (MQ=255)
                                                     gggCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTTTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGc                                                                                         <  1:282815/149‑1 (MQ=255)
                                                             cgcTCTTTTGCCTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGAc                                                                                 <  1:310397/149‑1 (MQ=255)
                                                                 ctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgag                                                                             >  1:235172/1‑149 (MQ=255)
                                                                    tttCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaaa                                                                          <  2:730354/149‑1 (MQ=255)
                                                                       cttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGc                                                                       <  1:1043292/149‑1 (MQ=255)
                                                                        gtcGGGCTGGGTGGGCGGGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGct                                                                      <  2:507607/148‑1 (MQ=255)
                                                                            gTCTGGGTGGCCTGGGGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAc                                                                  <  2:574900/149‑1 (MQ=255)
                                                                                      ccTTGCGGTCGGGCCACGGCGTGTTCACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg                                                        <  1:14771/149‑1 (MQ=255)
                                                                                       cTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCgg                                                       >  1:1016587/1‑149 (MQ=255)
                                                                                              tCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCCTG‑ATCGCGCTGGTTCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGAc                                                <  1:548456/149‑1 (MQ=255)
                                                                                                   ccACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAc                                           >  1:745930/1‑149 (MQ=255)
                                                                                                   ccACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAc                                           >  2:143232/1‑149 (MQ=255)
                                                                                                   ccACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAc                                           >  1:815661/1‑149 (MQ=255)
                                                                                                   ccACGGCGTGTTAACCGAACTGGAAGGAAGCGCCGCAGCGCCACGAAGGGG‑CAGGAAACGGTTATTGCTCAATCGGCTTTATGAGGTCGCGGAGAGTAATGTGCGGCCGGACTTAGAAATTCCAGAGCATCGCGTCGGTACTGACATaa                                            >  2:385446/1‑149 (MQ=255)
                                                                                                    cACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAcc                                          <  2:728545/149‑1 (MQ=255)
                                                                                                     aCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATAAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAccc                                         >  1:732865/1‑149 (MQ=255)
                                                                                                      ctgCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                        <  2:1131400/147‑1 (MQ=255)
                                                                                                         cGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgct                                     <  1:1086700/149‑1 (MQ=255)
                                                                                                           tgtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGAACAGGAC‑TTGCGCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCGGACGGAGAAATGCCCTACCAGCTCTTCGGTACCGACATAACCctgctga                                   >  2:336419/1‑148 (MQ=255)
                                                                                                                  cGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCa                            <  2:503255/149‑1 (MQ=255)
                                                                                                                     aCTGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt                         <  1:298614/149‑1 (MQ=255)
                                                                                                                       tGGAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGGTCCGCCAGCTCATTGAGGTCTCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGATATAACCCTGCTGTTTAACCAGTTCg                       >  1:1028916/1‑149 (MQ=255)
                                                                                                                        ggAAGGCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGa                      <  1:686783/149‑1 (MQ=255)
                                                                                                                            ggCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataat                  >  1:5261/1‑149 (MQ=255)
                                                                                                                             gCAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataata                 <  1:43211/149‑1 (MQ=255)
                                                                                                                              cAGCGCCGCACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAc                <  2:854054/149‑1 (MQ=255)
                                                                                                                                  cccgcACCGCCATT‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGATGTCGCGGCGCATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGtt            <  1:479308/148‑1 (MQ=255)
                                                                                                                                     gcACCGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgtg         >  1:630256/1‑149 (MQ=255)
                                                                                                                                     gcACCGCCATG‑ATGGCGCAGGATCAGGTT‑GTGCTCCGCCAGCTCATTGAGGGCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgtg         <  2:616501/149‑1 (MQ=255)
                                                                                                                                         cGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTaa                               <  1:856648/123‑1 (MQ=255)
                                                                                                                                         cGCCATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTaa                               >  2:856648/1‑123 (MQ=255)
                                                                                                                                            cATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtgt  <  1:984426/149‑1 (MQ=255)
                                                                                                                                            cATG‑ATGGCGCAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtgt  >  2:909467/1‑149 (MQ=255)
                                                                                                                                                      |                                                                                                                                            
GCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGC‑CAGGATCAGGTT‑TTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGT  >  NC_000913_3_pae_tpiA/3560307‑3560594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A