Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F151 I1 R1 22 137.2 5304824 94.1% 4991839 128.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 2,981,457 T→G K242N (AAA→AAC)  araE ← arabinose transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA2,981,4570TG99.1% 374.7 / NA 108K242N (AAA→AAC) araEarabinose transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (55/52);  minor base A (0/1);  total (55/53)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

GTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAG  >  NC_000913_3_pae_tpiA/2981311‑2981603
                                                                                                                                                  |                                                                                                                                                  
gTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGgttt                                                                                                                                                 <  1:1196750/150‑1 (MQ=255)
gTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGgttt                                                                                                                                                 >  1:911422/1‑150 (MQ=255)
gTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGATCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGCGGTTACGGTGAATCTTAAACACTGCCCACTCGCGCTGgttt                                                                                                                                                 >  4:243108/1‑150 (MQ=255)
 tAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTa                                                                                                                                                >  3:149916/1‑150 (MQ=255)
   aaGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAAc                                                                                                                                              <  4:864264/150‑1 (MQ=255)
   aaGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAAc                                                                                                                                              <  4:2695/150‑1 (MQ=255)
    aGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACt                                                                                                                                             >  2:694144/1‑150 (MQ=255)
      cccGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTc                                                                                                                                           >  2:942169/1‑150 (MQ=255)
             ttttGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCttt                                                                                                                                    <  1:123693/150‑1 (MQ=255)
              tttGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTGTc                                                                                                                                   <  2:911422/150‑1 (MQ=255)
               ttGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCa                                                                                                                                  <  4:583894/150‑1 (MQ=255)
               ttGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCa                                                                                                                                  <  1:1325468/150‑1 (MQ=255)
               catgtactACGCGCCGCGTAGTACATGATGATGTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCa                                                                                                                                  >  2:369733/8‑150 (MQ=255)
                    gATACGCGGCGCGTAGTACATGATGATGTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAAt                                                                                                                             <  4:149916/150‑1 (MQ=255)
                       aCGCGGCGCGTAGTACATGATGATGTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTc                                                                                                                          >  3:277278/1‑150 (MQ=255)
                             cgcgTAGTACATGATGATGTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAg                                                                                                                    <  3:655174/150‑1 (MQ=255)
                                  agtaCATGATGATGTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACGTACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGttctt                                                                                                               <  4:866001/150‑1 (MQ=255)
                                       atgatgatgTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTcgc                                                                                                          <  1:942169/150‑1 (MQ=255)
                                       atgatgatgTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTcgc                                                                                                          <  1:1637892/150‑1 (MQ=255)
                                         gatgatgTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTtctc                                                                                                            >  3:232141/1‑146 (MQ=255)
                                         gatgatgTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCgcgc                                                                                                        <  4:942617/150‑1 (MQ=255)
                                            gatgTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCttt                                                                                                     >  4:630597/1‑150 (MQ=255)
                                            gatgTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCttt                                                                                                     <  1:369733/150‑1 (MQ=255)
                                              tgTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCTCAACTACCCAGGATTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCttttt                                                                                                   >  1:484593/1‑150 (MQ=255)
                                               gTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGa                                                                                                                               <  4:563289/121‑1 (MQ=255)
                                               gTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGa                                                                                                                               >  3:563289/1‑121 (MQ=255)
                                               gTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTCCAGGCTTTCACGAATTTCGTTGAGTTCATCTCGCGCTTTTTc                                                                                                  >  2:682591/1‑150 (MQ=255)
                                                ttCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTcc                                                                                                 <  1:694144/150‑1 (MQ=255)
                                                         gTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAAc                                                                                                                                              >  1:690211/1‑96 (MQ=255)
                                                         gTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAAc                                                                                                                                              <  2:690211/96‑1 (MQ=255)
                                                         gTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGa                                                                                               <  4:986201/143‑1 (MQ=255)
                                                         gTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGa                                                                                               >  3:986201/1‑143 (MQ=255)
                                                                 ctgcATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCa                                                                                                                                  >  2:1012413/1‑100 (MQ=255)
                                                                 ctgcATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCa                                                                                                                                  <  1:1012413/100‑1 (MQ=255)
                                                                    cATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACg                                                                             >  1:968918/1‑150 (MQ=255)
                                                                      tCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGACTTTCGTTGAGTTCTTCGCGCGCTTTTTTCGACGTATCTCGCACCATCCGCa                                                                           >  2:1289396/1‑150 (MQ=255)
                                                                      tCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCa                                                                           >  3:430509/1‑150 (MQ=255)
                                                                      tCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGCTACGGTAAATCTTAAACAGTACCCAACCACCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGGTGTACTCGCGCTTTGTCCGCCGTATAGCGCAGCATACGCa                                                                           >  2:540580/1‑150 (MQ=255)
                                                                           tGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATAct                                                                      <  3:810358/150‑1 (MQ=255)
                                                                             caacaaCATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGc                                                                                                                                       <  2:669651/83‑1 (MQ=255)
                                                                             caacaaCATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGc                                                                                                                                       >  1:669651/1‑83 (MQ=255)
                                                                             caacaaCATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATActtc                                                                    >  3:715695/1‑150 (MQ=255)
                                                                              aacaacATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTc                                                                                                                                           <  2:132194/78‑1 (MQ=255)
                                                                              aacaacATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGtt                                                                                                                       <  1:1254587/98‑1 (MQ=255)
                                                                                caacaTACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACttcttc                                                                 <  2:1519527/150‑1 (MQ=255)
                                                                                  acaTACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCg                                                               <  2:1210780/150‑1 (MQ=255)
                                                                                   caTACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGc                                                              >  2:368908/1‑150 (MQ=255)
                                                                                   caTACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGc                                                              <  3:630597/150‑1 (MQ=255)
                                                                                     tACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCt                                                            >  4:393503/1‑150 (MQ=255)
                                                                                        cGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAg                                                                                                                                         >  4:747702/1‑70 (MQ=255)
                                                                                        cGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAg                                                                                                                                         <  3:747702/70‑1 (MQ=255)
                                                                                        cGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGTGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCCCaa                                                         >  4:906239/1‑150 (MQ=255)
                                                                                        cGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCGGCATACGCAATACTTCTTCCGCCTCaa                                                         >  1:1265365/1‑150 (MQ=255)
                                                                                         gagaAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCttt                                                                                                                                    >  4:799048/1‑74 (MQ=255)
                                                                                                acaGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGc                                                 >  1:1082505/1‑150 (MQ=255)
                                                                                                    cACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATActtc                                                                    >  1:1479796/1‑127 (MQ=255)
                                                                                                    cACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATActtc                                                                    <  2:1479796/127‑1 (MQ=255)
                                                                                                    cACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCt                                             >  1:367782/1‑150 (MQ=255)
                                                                                                       gacggacgTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCtttt                                          >  3:903196/1‑150 (MQ=255)
                                                                                                         cggacgTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTcc                                        <  2:1494667/150‑1 (MQ=255)
                                                                                                          ggacgTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCg                                       >  4:357513/1‑150 (MQ=255)
                                                                                                           gacgTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCttt                                           <  3:158197/145‑1 (MQ=255)
                                                                                                           gacgTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCttt                                           >  4:158197/1‑145 (MQ=255)
                                                                                                                tACGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCgccagcc                                 >  1:164656/1‑150 (MQ=255)
                                                                                                                 aCGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCgccagcca                                <  3:357513/150‑1 (MQ=255)
                                                                                                                  cGGTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGccagccag                               >  1:77066/1‑150 (MQ=255)
                                                                                                                   ggTTGATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCcagccagc                              >  3:5067/1‑150 (MQ=255)
                                                                                                                       gATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCgcc                                     >  3:169067/1‑139 (MQ=255)
                                                                                                                       gATCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCgcc                                     <  4:169067/139‑1 (MQ=255)
                                                                                                                         tCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTcgcg                                                                                                         >  1:591767/1‑69 (MQ=255)
                                                                                                                         tCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTcgcg                                                                                                         <  2:591767/69‑1 (MQ=255)
                                                                                                                         tCTTAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCAGCCTCAATATGACGCCCCTTTTCCGCCAGCCTGCGCgggg                        >  2:753738/1‑149 (MQ=255)
                                                                                                                           ttAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTa                      >  3:927447/1‑150 (MQ=255)
                                                                                                                           ttAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTa                      >  1:1459052/1‑150 (MQ=255)
                                                                                                                           ttAAACAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTa                      >  1:1270418/1‑150 (MQ=255)
                                                                                                                             aaaCAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATActtc                                                                    >  1:1250269/1‑102 (MQ=255)
                                                                                                                             aaaCAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATActtc                                                                    <  2:1250269/102‑1 (MQ=255)
                                                                                                                              aaCAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTAttt                   <  1:368908/150‑1 (MQ=255)
                                                                                                                               aCAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGccc                                               <  3:543235/121‑1 (MQ=255)
                                                                                                                               aCAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGccc                                               >  4:543235/1‑121 (MQ=255)
                                                                                                                                cAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTTTCGTTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTgg                 <  1:29373/150‑1 (MQ=255)
                                                                                                                                cAGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTgg                 <  2:1082505/150‑1 (MQ=255)
                                                                                                                                 aGTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGc                <  2:164656/150‑1 (MQ=255)
                                                                                                                                  gTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCa               <  1:277059/150‑1 (MQ=255)
                                                                                                                                  gTGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCa               <  4:596599/150‑1 (MQ=255)
                                                                                                                                   tGCCCAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAg              <  2:1270418/150‑1 (MQ=255)
                                                                                                                                     cccAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGa            >  1:261557/1‑150 (MQ=255)
                                                                                                                                      ccAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCg                                                                                                >  3:288202/1‑65 (MQ=255)
                                                                                                                                      ccAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCg                                                                                                <  4:288202/65‑1 (MQ=255)
                                                                                                                                      ccAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGaa           >  2:297989/1‑150 (MQ=255)
                                                                                                                                      ccAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGaa           <  4:903196/150‑1 (MQ=255)
                                                                                                                                       cAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACg                                                  <  2:1490813/110‑1 (MQ=255)
                                                                                                                                       cAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACg                                                  >  1:1490813/1‑110 (MQ=255)
                                                                                                                                       cAACCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAg          >  1:1047185/1‑150 (MQ=255)
                                                                                                                                        aaCCGCCCTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGa         <  1:1289396/150‑1 (MQ=255)
                                                                                                                                             cccTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTcg                                                                                                           >  3:60997/1‑47 (MQ=255)
                                                                                                                                              ccTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTAtt                    <  3:993851/133‑1 (MQ=255)
                                                                                                                                              ccTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCa   >  1:379332/1‑150 (MQ=255)
                                                                                                                                              ccTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCa   <  2:1047185/150‑1 (MQ=255)
                                                                                                                                               cTGGTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAg  >  4:179654/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAG  >  NC_000913_3_pae_tpiA/2981311‑2981603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A