Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A42 F60 I1 R1
|
15 |
25.4 |
857350 |
99.2% |
850491 |
139.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,432,016 |
T→C |
intergenic (‑33/+75) |
ytfE ← / ← ytfF |
iron‑sulfur cluster repair protein YtfE/inner membrane protein YtfF |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,432,016 | 0 | T | C | 100.0%
| 95.9
/ NA
| 26 | intergenic (‑33/+75) | ytfE/ytfF | iron‑sulfur cluster repair protein YtfE/inner membrane protein YtfF |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/13); total (13/13) |
ACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGACAGATTCAGTTAAAGGTTTTTCTGGTTTTGCTCTGACCGCG > NC_000913/4431885‑4432145
|
aCCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAAGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTaaa < 2:397634/140‑1 (MQ=255)
tCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCaa < 2:95034/140‑1 (MQ=255)
ccATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCaaa < 2:348911/140‑1 (MQ=255)
tatcatatTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGc > 1:252558/1‑140 (MQ=255)
tatcatatTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGc > 2:157022/1‑140 (MQ=255)
catatTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCtat < 1:281804/140‑1 (MQ=255)
catatTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCtat < 2:205587/140‑1 (MQ=255)
cGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTa > 2:407105/1‑140 (MQ=255)
agCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCag > 1:194215/1‑140 (MQ=255)
aaGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCt > 2:382712/1‑140 (MQ=255)
cgAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTAttt > 2:310426/1‑140 (MQ=255)
cgcCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGc > 1:405908/1‑140 (MQ=255)
gTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTgaga < 1:157022/140‑1 (MQ=255)
ttGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGAc > 1:212377/1‑140 (MQ=255)
ggTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGACAg > 2:330611/1‑140 (MQ=255)
gTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGACAGa > 1:38083/1‑140 (MQ=255)
gTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGACAGa > 1:308989/1‑140 (MQ=255)
cgcgATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGACAGAtt < 1:407105/140‑1 (MQ=255)
cgcgATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGACAGAtt < 1:310426/140‑1 (MQ=255)
tAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGACAGATTCAGtt < 1:382712/140‑1 (MQ=255)
tAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGACAGATTCAGtt < 2:308989/140‑1 (MQ=255)
tGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGACAGATTCAGTTAAAGGTTTTTc < 2:405908/140‑1 (MQ=255)
gATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGACAGATTCAGTTAAAGGTTTTTCt > 1:245546/1‑140 (MQ=255)
tACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGACAGATTCAGTTAAAGGTTTTTCTgg > 2:126168/1‑140 (MQ=255)
agataagatGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGACAGATTCAGTTAAAGGTTTTTCTGGTTTTGCTCTGACcgcg < 2:194215/140‑1 (MQ=255)
agataagatGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGACAGATTCAGTTAAAGGTTTTTCTGGTTTTGCTCTGACcgcg < 1:126168/140‑1 (MQ=255)
|
ACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGACAGATTCAGTTAAAGGTTTTTCTGGTTTTGCTCTGACCGCG > NC_000913/4431885‑4432145
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
