Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F60 I1 R1	11	63.9	2153418	99.3%	2138344	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,354,487	C→T	intergenic (‑437/‑238)	yhcC ← / → gltB	radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,354,487	0	C	T	100.0%	208.6 / NA	59	intergenic (‑437/‑238)	yhcC/gltB	radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (28/31); total (28/31)

GTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAG  >  NC_000913/3354351‑3354609

gTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGc                                                                                                                         >  2:496712/1‑140 (MQ=255)

gTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGc                                                                                                                         >  2:237837/1‑140 (MQ=255)

gTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGc                                                                                                                         >  2:389608/1‑140 (MQ=255)

   aGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCttt                                                                                                                      >  2:613788/1‑140 (MQ=255)

           aCTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATaa                                                                                                              >  2:720527/1‑140 (MQ=255)

           aCTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATaa                                                                                                              >  2:1074732/1‑140 (MQ=255)

           aCTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATaa                                                                                                              >  2:14960/1‑140 (MQ=255)

             tGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGt                                                                                                            <  2:81524/140‑1 (MQ=255)

             tGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGt                                                                                                            <  2:19880/140‑1 (MQ=255)

               tttCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTg                                                                                                          >  1:907028/1‑140 (MQ=255)

                 tctcTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGa                                                                                                        <  2:510970/140‑1 (MQ=255)

                     tAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATc                                                                                                    >  1:252043/1‑140 (MQ=255)

                         gACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCt                                                                                                >  2:659103/1‑140 (MQ=255)

                           cTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGTTTTCCCGATAAGTTGGAAATCCGCTgg                                                                                              >  2:575308/1‑140 (MQ=255)

                             tCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGaa                                                                                            >  1:184604/1‑140 (MQ=255)

                              cccTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAg                                                                                           >  2:439682/1‑140 (MQ=255)

                              cccTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTGTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAg                                                                                           <  1:1012397/140‑1 (MQ=255)

                                    tAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCt                                                                                     >  1:465566/1‑140 (MQ=255)

                                         ttAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATg                                                                                >  1:255027/1‑140 (MQ=255)

                                          tAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGa                                                                               <  1:529149/140‑1 (MQ=255)

                                            aaGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGAtagca                                                                             >  1:703012/1‑136 (MQ=255)

                                               aTAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGagcagc                                                                          <  1:439682/140‑1 (MQ=255)

                                                 aaaaTCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCt                                                                        <  1:210412/140‑1 (MQ=255)

                                                  aaaTCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTg                                                                       <  1:744131/140‑1 (MQ=255)

                                                   aaTCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGc                                                                      <  1:720527/140‑1 (MQ=255)

                                                    aTCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCt                                                                     <  1:400124/140‑1 (MQ=255)

                                                       cATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCtcat                                                                  <  1:580446/140‑1 (MQ=255)

                                                         ttttAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCtcatca                                                                <  1:14960/140‑1 (MQ=255)

                                                              aTTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATAttt                                                           >  2:983931/1‑140 (MQ=255)

                                                               tttCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTa                                                          <  2:255027/140‑1 (MQ=255)

                                                                 tCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATg                                                        <  1:215164/140‑1 (MQ=255)

                                                                 tCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATg                                                        <  1:576129/140‑1 (MQ=255)

                                                                 tCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATg                                                        <  1:613788/140‑1 (MQ=255)

                                                                   aGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCGTATTTATGCa                                                      <  1:949457/140‑1 (MQ=255)

                                                                   aGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCa                                                      >  1:1052632/1‑140 (MQ=255)

                                                                   aGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCa                                                      >  1:144947/1‑140 (MQ=255)

                                                                       aTTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTaa                                                  <  1:496712/140‑1 (MQ=255)

                                                                              aaaGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGAt                                           <  1:27454/140‑1 (MQ=255)

                                                                                 gAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATccc                                        >  2:78196/1‑140 (MQ=255)

                                                                                   aTTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCct                                      <  2:184604/140‑1 (MQ=255)

                                                                                   aTTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCct                                      <  2:907028/140‑1 (MQ=255)

                                                                                       tGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTc                                  <  2:1052632/140‑1 (MQ=255)

                                                                                        gcgcTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCa                                 <  1:875994/140‑1 (MQ=255)

                                                                                        gcgcTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCa                                 <  2:905251/140‑1 (MQ=255)

                                                                                        gcgcTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCa                                 <  1:237837/140‑1 (MQ=255)

                                                                                         cgcTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCAc                                >  1:300885/1‑140 (MQ=255)

                                                                                               aGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTAtt                          <  1:983931/140‑1 (MQ=255)

                                                                                                    aTTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCg                     <  1:297848/140‑1 (MQ=255)

                                                                                                      ttCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGAt                   >  1:332710/1‑140 (MQ=255)

                                                                                                      ttCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGAt                   <  2:701646/140‑1 (MQ=255)

                                                                                                           tACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGaa              >  1:79545/1‑140 (MQ=255)

                                                                                                                aTAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGa         >  2:401819/1‑140 (MQ=255)

                                                                                                                 tAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGac        >  2:310661/1‑140 (MQ=255)

                                                                                                                   aGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGacaa      >  2:583924/1‑140 (MQ=255)

                                                                                                                    gCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGacaac     <  2:465566/140‑1 (MQ=255)

                                                                                                                       tGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAg  <  2:252043/140‑1 (MQ=255)

                                                                                                                       tGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAg  >  1:1011442/1‑140 (MQ=255)

                                                                                                                        gCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGAt                   <  1:922491/122‑1 (MQ=255)

                                                                                                                        gCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGAt                   >  2:922491/1‑122 (MQ=255)

GTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAG  >  NC_000913/3354351‑3354609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A