Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A44 F60 I1 R1 18 142.5 4932706 99.3% 4898177 138.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,731,550 T→C intergenic (‑393/+50) rrsG ← / ← clpB 16S ribosomal RNA/ClpB chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,731,5500TC100.0% 236.0 / NA 60intergenic (‑393/+50)rrsG/clpB16S ribosomal RNA/ClpB chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/29);  total (31/29)

CCCGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGT  >  NC_000913/2731415‑2731684
                                                                                                                                       |                                                                                                                                      
cccGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGt                                                                                                                                    <  1:951708/140‑1 (MQ=255)
  cggcggCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCt                                                                                                                                  <  1:964444/140‑1 (MQ=255)
  cggcggCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCt                                                                                                                                  >  2:1669405/1‑140 (MQ=255)
  cggcggCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCt                                                                                                                                  <  1:1524838/140‑1 (MQ=255)
       gcgTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACtt                                                                                                                             <  1:1055923/140‑1 (MQ=255)
        cgTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTta                                                                                                                            <  1:2454067/140‑1 (MQ=255)
          tgtTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTtata                                                                                                                          >  2:577533/1‑140 (MQ=255)
          tgtTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTtata                                                                                                                          <  2:602830/140‑1 (MQ=255)
              tGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACa                                                                                                                      >  2:2393051/1‑140 (MQ=255)
                   ttgttCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAAc                                                                                                                 <  1:1391071/140‑1 (MQ=255)
                   ttgttCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAAc                                                                                                                 <  1:1534889/140‑1 (MQ=255)
                   ttgttCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGACAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAAc                                                                                                                 <  1:1412618/140‑1 (MQ=255)
                      ttCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAg                                                                                                              <  1:1502332/140‑1 (MQ=255)
                           tgtCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTtat                                                                                                                           >  2:236154/1‑122 (MQ=255)
                           tgtCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTtat                                                                                                                           <  1:236154/122‑1 (MQ=255)
                           tgtCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCg                                                                                                         >  1:973551/1‑140 (MQ=255)
                                gtggtgGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAggg                                                                                                    >  1:1562588/1‑140 (MQ=255)
                                gtggtgGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAggg                                                                                                    >  2:2078757/1‑140 (MQ=255)
                                    tggCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCg                                                                                                <  1:1669405/140‑1 (MQ=255)
                                     ggCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGt                                                                                               >  2:2434564/1‑140 (MQ=255)
                                      gcgcATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGtt                                                                                              <  2:2345521/140‑1 (MQ=255)
                                      gcgcATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGGCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGtt                                                                                              <  1:1892308/140‑1 (MQ=255)
                                       cgcATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAggg                                                                                                    <  2:2059514/133‑1 (MQ=255)
                                       cgcATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAggg                                                                                                    >  1:2059514/1‑133 (MQ=255)
                                                   aGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGc                                                                                                   >  1:1347158/1‑122 (MQ=255)
                                                   aGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGc                                                                                                   <  2:1347158/122‑1 (MQ=255)
                                                    gTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTgg                                                                                >  2:966480/1‑140 (MQ=255)
                                                    gTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACAAGCCCCGAAGGGCTCGTTTTATc                                                                                         >  1:1443514/1‑131 (MQ=255)
                                                    gTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACAAGCCCCGAAGGGCTCGTTTTATc                                                                                         <  2:1443514/131‑1 (MQ=255)
                                                      tATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGAc                                                                              >  1:2415079/1‑140 (MQ=255)
                                                               cTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAAt                                                                     <  1:2434564/140‑1 (MQ=255)
                                                                tGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATc                                                                    <  1:966480/140‑1 (MQ=255)
                                                                    aaGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGt                                                                <  2:181160/140‑1 (MQ=255)
                                                                     aGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTc                                                               <  1:2393051/140‑1 (MQ=255)
                                                                       cGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCtt                                                             >  2:531801/1‑140 (MQ=255)
                                                                         aaaaaTATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCa                                                           >  2:571591/1‑140 (MQ=255)
                                                                                      cTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGc                                              >  1:545563/1‑140 (MQ=255)
                                                                                        ttATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGa                                            >  1:1662480/1‑140 (MQ=255)
                                                                                         tATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCAtt                                                         <  2:1945343/126‑1 (MQ=255)
                                                                                         tATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCAtt                                                         >  1:1945343/1‑126 (MQ=255)
                                                                                          aTCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAAt                                          >  2:402968/1‑140 (MQ=255)
                                                                                               tCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACtt                                     >  1:1035393/1‑140 (MQ=255)
                                                                                               tCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACtt                                     <  1:2078757/140‑1 (MQ=255)
                                                                                                             ggCAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAAt                       >  2:1774787/1‑140 (MQ=255)
                                                                                                               cAAAACATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTc                     <  1:577533/140‑1 (MQ=255)
                                                                                                                    cATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAg                <  2:1345266/140‑1 (MQ=255)
                                                                                                                    cATCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAg                <  1:402968/140‑1 (MQ=255)
                                                                                                                     aTCTTAAATATAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGa               <  2:2415079/140‑1 (MQ=255)
                                                                                                                            atatAGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTAtt        >  1:1853252/1‑140 (MQ=255)
                                                                                                                               taGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGc     >  2:2284675/1‑140 (MQ=255)
                                                                                                                                aGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGAc              <  1:1982591/130‑1 (MQ=255)
                                                                                                                                aGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGAc              >  2:1982591/1‑130 (MQ=255)
                                                                                                                                aGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGAc              >  2:816331/1‑130 (MQ=255)
                                                                                                                                aGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGAc              <  1:816331/130‑1 (MQ=255)
                                                                                                                                aGTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCt    >  2:422893/1‑140 (MQ=255)
                                                                                                                                 gTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCtg   >  1:2179076/1‑140 (MQ=255)
                                                                                                                                 gTCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCtg   >  1:1231652/1‑140 (MQ=255)
                                                                                                                                  tCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCtgt  >  2:1327711/1‑140 (MQ=255)
                                                                                                                                  tCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCtgt  >  1:386380/1‑140 (MQ=255)
                                                                                                                                  tCTTTCCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCtgt  >  1:931703/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                      
CCCGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGT  >  NC_000913/2731415‑2731684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A