Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	145	69.6	2346944	99.3%	2330515	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,680,542	G→A	A735V (GCG→GTG)	yphG ←	DUF5107 domain‑containing protein YphG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,680,542	0	G	A	100.0%	124.2 / NA	36	A735V (GCG→GTG)	yphG	DUF5107 domain‑containing protein YphG
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (28/8); total (28/8)

CTCCGGCGACATCTCTACGCAACGTTGCCAAAAGGCAATGGCTTTGTTGTAGCTACGTTTGTTGTAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCGCCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCGTGCGCTGGCAGTCCAG  >  NC_000913/2680418‑2680678

cTCCGGCGACATCTCTACGCAACGTTGCCAAAAGGCAATGGCTTTGTTGTAGCTACGTTTGTTGTAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCg                                                                                                                           <  2:243625/140‑1 (MQ=255)

           tctcTACGCAACGTTGCCAAAAGGCAATGGCTTTGTTGTAGCTACGTTTGTTGTAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGaaa                                                                                                                <  1:626772/140‑1 (MQ=255)

                     aCGTTGCCAAAAGGCAATGGCTTTGTTGTAGCTACGTTTGTTGTAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACt                                                                                                      >  1:38215/1‑140 (MQ=255)

                         tGCCAAAAGGCAATGGCTTTGTTGTAGCTACGTTTGTTGTAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCgg                                                                                                  >  1:900286/1‑140 (MQ=255)

                           ccAAAAGGCAATGGCTTTGTTGTAGCTACGTTTGTTGTAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGa                                                                                                >  1:114926/1‑140 (MQ=255)

                                            tgttgtAGCTACGTTTGTTGTAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCtttt                                                                               <  2:114926/140‑1 (MQ=255)

                                                 tAGCTACGTTTGTTGTAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGAc                                                                          <  2:347132/140‑1 (MQ=255)

                                                      aCGTTTGTTGTAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGtt                                                                     >  1:442069/1‑140 (MQ=255)

                                                      aCGTTTGTTGTAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTAGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGtt                                                                     >  1:443628/1‑140 (MQ=255)

                                                        gTTTGTTGTAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCg                                                                   <  2:900286/140‑1 (MQ=255)

                                                        gTTTGTTGTAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCg                                                                   >  1:966088/1‑140 (MQ=255)

                                                              tgtAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGcc                                                                 <  2:502664/136‑1 (MQ=255)

                                                              tgtAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGcc                                                                 >  1:502664/1‑136 (MQ=255)

                                                                 agtagAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCg                                                          >  1:845069/1‑140 (MQ=255)

                                                                  gtagAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCgg                                                         >  1:397652/1‑140 (MQ=255)

                                                                      aagcaagcTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCttt                                                     >  2:964763/1‑140 (MQ=255)

                                                                             cTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGgcagc                                              >  1:585640/1‑140 (MQ=255)

                                                                                gTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAg                                           <  2:966088/140‑1 (MQ=255)

                                                                                    aTGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTCCGCCA‑CAAGCTGGc                                      >  2:565967/1‑140 (MQ=255)

                                                                                          aGCAAACCAGCACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCa                                 >  1:882032/1‑140 (MQ=255)

                                                                                                    cACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCgg                       >  1:1070182/1‑140 (MQ=255)

                                                                                                    cACTCTTCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCgg                       >  1:169773/1‑140 (MQ=255)

                                                                                                         ttCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCg                  >  1:624451/1‑140 (MQ=255)

                                                                                                         ttCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCg                  >  1:135030/1‑140 (MQ=255)

                                                                                                         ttCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCg                  >  1:256563/1‑140 (MQ=255)

                                                                                                         ttCAATACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCg                  >  2:786077/1‑140 (MQ=255)

                                                                                                             aTACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCGTgcg              >  2:1054900/1‑140 (MQ=255)

                                                                                                              tACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCGTgcgc             >  1:555667/1‑140 (MQ=255)

                                                                                                              tACTCTCCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCGTgcgc             <  2:135030/140‑1 (MQ=255)

                                                                                                                 tctcCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCGTGCGCTgg          >  2:712366/1‑140 (MQ=255)

                                                                                                                 tctcCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCGTGCGCTgg          >  2:45322/1‑140 (MQ=255)

                                                                                                                   tcCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCGTGCGCTGGCa        >  1:641634/1‑140 (MQ=255)

                                                                                                                   tcCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCGTGCGCTGGCa        >  1:1147123/1‑140 (MQ=255)

                                                                                                                   tcCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCGTGCGCTGGCa        >  2:257302/1‑140 (MQ=255)

                                                                                                                   tcCAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCGTGCGCTGGCa        >  1:319428/1‑140 (MQ=255)

                                                                                                                     cAGCGCCACCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCGTGCGCTGGCAGt      >  2:411707/1‑140 (MQ=255)

                                                                                                                        cgccacCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCGTGCGCTGGCAGTCCa   >  2:372977/1‑140 (MQ=255)

                                                                                                                         gccaccACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCGTGCGCTGGCAGTCCAg  <  2:1070182/140‑1 (MQ=255)

CTCCGGCGACATCTCTACGCAACGTTGCCAAAAGGCAATGGCTTTGTTGTAGCTACGTTTGTTGTAGTAGAAGCAAGCTAGTAAATGGCGAGCAAACCAGCACTCTTCAATACTCTCCAGCGCCGCCACTTCTTCCAGCGTATTCGGGAAACGGACAAACTGCGGGAAGACATCAATGGCTTTTGCGACCAGTTCGCCACGTTCGGCTTTCGGCAGCAAGCTGGCTTGCAGGTAAAGCGGCAGCGTGCGCTGGCAGTCCAG  >  NC_000913/2680418‑2680678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A