Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	136	55.1	1935284	95.6%	1850131	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,698,405	G→A	A49V (GCC→GTC)	pgpC ←	phosphatidylglycerophosphatase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,698,405	0	G	A	100.0%	161.9 / NA	46	A49V (GCC→GTC)	pgpC	phosphatidylglycerophosphatase C
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/24); total (22/24)

ACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGGCTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCT  >  NC_000913/2698270‑2698537

aCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACtat                                                                                                                                  >  1:818514/1‑140 (MQ=255)

 cATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACtata                                                                                                                                 <  2:818514/140‑1 (MQ=255)

 cATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACtata                                                                                                                                 <  2:152211/140‑1 (MQ=255)

    tGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATc                                                                                                                              <  1:525008/140‑1 (MQ=255)

                  cgcACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAg                                                                                                                >  2:281272/1‑140 (MQ=255)

                            cGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAAc                                                                                                      >  1:361956/1‑140 (MQ=255)

                                    aaCGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAg                                                                                              <  2:330064/140‑1 (MQ=255)

                                    aaCGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCACACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAg                                                                                              <  1:335356/140‑1 (MQ=255)

                                           gTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTgg                                                                                       <  2:361956/140‑1 (MQ=255)

                                           gTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTgg                                                                                       <  1:909664/140‑1 (MQ=255)

                                             aaaCGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCg                                                                                     <  1:281272/140‑1 (MQ=255)

                                             aaaCGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCg                                                                                     <  2:797887/140‑1 (MQ=255)

                                             aaaCGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCg                                                                                     <  1:832324/140‑1 (MQ=255)

                                             aaaCGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCg                                                                                     <  1:154815/140‑1 (MQ=255)

                                             aaaCGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAACAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCg                                                                                     <  2:672482/140‑1 (MQ=255)

                                                cGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACg                                                                                  >  2:636616/1‑140 (MQ=255)

                                                  tGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCg                                                                                     >  2:571952/1‑135 (MQ=255)

                                                  tGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCg                                                                                     <  1:788611/135‑1 (MQ=255)

                                                  tGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCg                                                                                     <  1:571952/135‑1 (MQ=255)

                                                  tGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCg                                                                                     >  2:788611/1‑135 (MQ=255)

                                                  tGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGAc                                                                                   >  2:405342/1‑137 (MQ=255)

                                                  tGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGAc                                                                                   <  1:405342/137‑1 (MQ=255)

                                                   gCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACgtag                                                                               >  2:707710/1‑140 (MQ=255)

                                                    cTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACgtagt                                                                              >  2:77975/1‑140 (MQ=255)

                                                    cTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACgtagt                                                                              >  2:713205/1‑140 (MQ=255)

                                                      tCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCg                                                                                     >  1:416753/1‑131 (MQ=255)

                                                      tCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCg                                                                                     <  2:416753/131‑1 (MQ=255)

                                                            tgACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAgcg                                                                      <  1:636616/140‑1 (MQ=255)

                                                              aCCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCa                                                                    >  1:303007/1‑140 (MQ=255)

                                                                      tGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTg                                                            <  1:747044/140‑1 (MQ=255)

                                                                      tGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTg                                                            <  1:713205/140‑1 (MQ=255)

                                                                        cACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGcc                                                          >  1:509737/1‑140 (MQ=255)

                                                                                  gAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATcc                                                <  1:34869/140‑1 (MQ=255)

                                                                                         aCTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGAt                                         >  1:584348/1‑140 (MQ=255)

                                                                                             aTCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCaa                                     >  2:415715/1‑140 (MQ=255)

                                                                                             aTCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCaa                                     >  2:504031/1‑140 (MQ=255)

                                                                                                   cAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCctgctg                                           >  1:957589/1‑128 (MQ=255)

                                                                                                   cAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCctgctg                                           <  2:957589/128‑1 (MQ=255)

                                                                                                     gCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCa                                      <  2:776161/131‑1 (MQ=255)

                                                                                                     gCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCa                                      >  1:776161/1‑131 (MQ=255)

                                                                                                                     tttATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAacac             <  2:370188/140‑1 (MQ=255)

                                                                                                                          cAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAAAGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGTTGCCATGTTCCCTCTAAGTCAAAAAACACCacac        >  2:561075/1‑140 (MQ=255)

                                                                                                                            aTAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACGCCACacga      <  1:561075/140‑1 (MQ=255)

                                                                                                                            aTAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACacga      >  2:743299/1‑140 (MQ=255)

                                                                                                                                cGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCt  >  2:522827/1‑140 (MQ=255)

                                                                                                                                cGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCt  >  2:119139/1‑140 (MQ=255)

ACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGGCTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCT  >  NC_000913/2698270‑2698537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A