Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	114	64.8	2195608	99.1%	2175847	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,701,467	G→A	P88L (CCA→CTA)	pdxJ ←	pyridoxine 5'‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,701,467	0	G	A	100.0%	164.3 / NA	47	P88L (CCA→CTA)	pdxJ	pyridoxine 5'‑phosphate synthase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/25); total (22/25)

AACAGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACG  >  NC_000913/2701332‑2701601

aaCAGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCtt                                                                                                                                    <  2:273250/140‑1 (MQ=255)

aaCAGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCtt                                                                                                                                    <  1:687062/140‑1 (MQ=255)

     agaAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGtct                                                                                                                               >  1:1056077/1‑140 (MQ=255)

     agaAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGAACCAGGCAGCAAAAATGTAGCTTCGtct                                                                                                                               >  2:734223/1‑140 (MQ=255)

      gaAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGtctc                                                                                                                              >  2:971752/1‑140 (MQ=255)

      gaAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGtctc                                                                                                                              >  2:810093/1‑140 (MQ=255)

      gaAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGtctc                                                                                                                              >  2:576943/1‑140 (MQ=255)

       aaaCCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCa                                                                                                                             >  2:797325/1‑140 (MQ=255)

        aaCCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCaa                                                                                                                            <  1:810093/140‑1 (MQ=255)

                   cGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGcc                                                                                                                 <  1:699740/140‑1 (MQ=255)

                     gCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAg                                                                                                               <  1:971752/140‑1 (MQ=255)

                            ccAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATctct                                                                                                        >  1:977607/1‑140 (MQ=255)

                                   tttGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCa                                                                                                 >  1:452363/1‑140 (MQ=255)

                                   tttGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCa                                                                                                 <  2:452363/140‑1 (MQ=255)

                                     tGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCAcc                                                                                               >  2:988329/1‑140 (MQ=255)

                                        aGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGcc                                                                                            <  1:576943/140‑1 (MQ=255)

                                        aGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGcc                                                                                            <  1:26080/140‑1 (MQ=255)

                                              cgcgCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTcc                                                                                      >  2:86047/1‑140 (MQ=255)

                                              cgcgCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTcc                                                                                      >  1:1091090/1‑140 (MQ=255)

                                              cgcgCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTcc                                                                                      >  2:236675/1‑140 (MQ=255)

                                                         tCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCg                                                                           >  2:828432/1‑140 (MQ=255)

                                                                 cccTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCa                                                                   >  2:137912/1‑140 (MQ=255)

                                                                     gCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGt                                                               <  2:657976/140‑1 (MQ=255)

                                                                       gACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCt                                                             <  2:977607/140‑1 (MQ=255)

                                                                           tCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACg                                                         >  1:9515/1‑140 (MQ=255)

                                                                           tCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACg                                                         >  2:739811/1‑140 (MQ=255)

                                                                             cAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCCTCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCa                                                       <  2:1056077/140‑1 (MQ=255)

                                                                                     cTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGGTgcgc                                               >  1:576231/1‑140 (MQ=255)

                                                                                     cTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATgcgc                                               >  1:980656/1‑140 (MQ=255)

                                                                                      ttCGGTTGTTACTTGCTGACGCTTTTCCGGCACCAGGCAGCAAAACTGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGAGGCGCa                                              >  2:816295/1‑140 (MQ=255)

                                                                                          gttgttACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTc                                          <  2:551015/140‑1 (MQ=255)

                                                                                            tgttACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcgc                                        <  1:988329/140‑1 (MQ=255)

                                                                                            tgttACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcgc                                        <  2:625784/140‑1 (MQ=255)

                                                                                             gttACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcgcg                                       <  1:325490/140‑1 (MQ=255)

                                                                                             gttACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcgcg                                       <  1:426035/140‑1 (MQ=255)

                                                                                               tACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGt                                     <  2:563143/140‑1 (MQ=255)

                                                                                               tACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGt                                     <  2:698126/140‑1 (MQ=255)

                                                                                                    ccTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTa                                >  2:305679/1‑140 (MQ=255)

                                                                                                     cTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcgc                                        >  2:94548/1‑131 (MQ=255)

                                                                                                     cTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcgc                                        <  1:94548/131‑1 (MQ=255)

                                                                                                     cTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTaa                               >  1:934968/1‑140 (MQ=255)

                                                                                                               tCCGGTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCg                     >  1:829656/1‑140 (MQ=255)

                                                                                                                  ggTACCAGGCAGCAAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATc                  <  1:737221/140‑1 (MQ=255)

                                                                                                                              caAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATg      <  1:797325/140‑1 (MQ=255)

                                                                                                                              caAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATg      >  1:189375/1‑140 (MQ=255)

                                                                                                                              caAAAATGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATg      <  1:137912/140‑1 (MQ=255)

                                                                                                                                aaaaTGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCa    <  2:9515/140‑1 (MQ=255)

                                                                                                                                aaaaTGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCa    <  2:1091090/140‑1 (MQ=255)

                                                                                                                                  aaTGTAGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACg  <  1:734223/140‑1 (MQ=255)

AACAGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACG  >  NC_000913/2701332‑2701601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A